Abstract:
:Subcellular fractionation revealed that a significant fraction of total sphingosine kinase, the enzyme that phosphorylates sphingosine to form the bioactive lipid metabolite sphingosine-1-phosphate, resides in the nuclei of Swiss 3T3 cells, localized to both the nuclear envelope and the nucleoplasm. Platelet-derived growth factor, in addition to rapidly stimulating cytosolic sphingosine kinase, also induced a large increase in nucleoplasm-associated activity after 12-24 h that correlated with progression of cells to the S-phase of the cell cycle and translocation of sphingosine kinase-green fluorescent protein fusion protein to the nuclear envelope. Our results add sphingosine kinase to the growing list of lipid-metabolizing enzymes associated with the nucleus, and suggest that sphingosine-1-phosphate may also play a role in signal transduction in the nucleus.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kleuser B,Maceyka M,Milstien S,Spiegel Sdoi
10.1016/s0014-5793(01)02697-7keywords:
subject
Has Abstractpub_date
2001-08-10 00:00:00pages
85-90issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(01)02697-7journal_volume
503pub_type
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