Abstract:
:The predominance of oxidative stress in many tumour cell environments provides a means to selectively target these cells via protein oxidation. The zinc fingers of transcription factors utilise cysteine thiols for structural zinc coordination. Redox control of DNA binding regulates transcription and therefore the overall rates of proliferation, apoptosis and necrosis in the carcinoma. We report here the adverse effects of glutathione peroxidase (GPx) mimics towards zinc finger motifs and PC12 cell survival. Nanomolar catalyst concentrations facilitated H2O2-induced oxidation of an Sp1 transcription factor fragment. In PC12 cells GPx catalysis triggered a significant increase in cell death, correlating with severity of oxidative stress. As a consequence, we conclude that GPx mimics are potential chemotherapeutic agents.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Giles NM,Gutowski NJ,Giles GI,Jacob Cdoi
10.1016/s0014-5793(02)03890-5keywords:
subject
Has Abstractpub_date
2003-01-30 00:00:00pages
179-82issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579302038905journal_volume
535pub_type
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