The DNA sequence specificity of cyanomorpholinoadriamycin.

abstract：:To assess the role of crystallinity in biological response, we quantitated the generation of reactive oxygen metabolites in human polymorphonuclear leukocytes (PMN) with a chemiluminescence assay using three hydroxyapatite preparations with sintering temperatures of 1,200 degrees C and 900 degrees C and a drying tempe...

journal_title：FEBS letters

authors： Nagase M,Nishiya H,Abe Y

更新日期：1993-07-05 00:00:00

abstract：:The effect of artificial model membranes on the complement system was investigated. Incubation of the model membranes with human serum resulted in consumption of complement haemolytic activity when phosphatidylserine-containing vesicles were used. The activation of the complement system appeared to proceed through the...

journal_title：FEBS letters

authors： Comis A,Easterbrook-Smith SB

更新日期：1986-03-03 00:00:00

abstract：:A synthetic peptide (23 residues) that includes the antibacterial and lipopolysaccharide-binding regions of human lactoferricin, an antimicrobial sequence of lactoferrin, was used to study its action on cytoplasmic membrane of Escherichia coli 0111 and E. coli phospholipid vesicles. The peptide caused a depolarization...

journal_title：FEBS letters

authors： Aguilera O,Ostolaza H,Quirós LM,Fierro JF

更新日期：1999-12-03 00:00:00

abstract：:Parasitic helminths remain major pathogens of both humans and animals throughout the world. The success of helminth infections depends on the capacity of the parasite to counteract host immune responses but also to exploit host-derived signal molecules for its development. Recent progress has been made in the characte...

journal_title：FEBS letters

authors： Dissous C,Khayath N,Vicogne J,Capron M

更新日期：2006-05-22 00:00:00

abstract：:The expression of myf-5, a key component of myogenic regulatory genes, declines progressively in mature somitic cells during vertebrate myogenesis. Little is known about how this down-regulation takes place. Here we provide evidence that an interferon regulatory factor binding element (IRF element) within the Xenopus ...

journal_title：FEBS letters

authors： Mei W,Yang J,Tao Q,Geng X,Rupp RA,Ding X

更新日期：2001-09-07 00:00:00

abstract：:Human CBS1 is a methionine sulfoxide reductase of type B (MSRB) as it specifically reduced Met-R-SO in peptides with dithiothreitol or the thioredoxin system as reductants. Mutation C169S in the active site completely abolished enzymatic activity, while mutation W110A only reduced activity and C105S had no effect. Lik...

journal_title：FEBS letters

authors： Jung S,Hansel A,Kasperczyk H,Hoshi T,Heinemann SH

更新日期：2002-09-11 00:00:00

abstract：:Casein phosphopeptides beta-CN(1-25)4P and alpha(s1)-CN(59-79)5P, from beta- and alpha(s1)-casein, respectively, both carrying the characteristic 'acidic motif' Ser(P)-Ser(P)-Ser(P)-Glu-Glu, were chemically synthesized and administered to HT-29 cells differentiated in culture, which are a used model of intestinal epit...

journal_title：FEBS letters

authors： Ferraretto A,Gravaghi C,Fiorilli A,Tettamanti G

更新日期：2003-09-11 00:00:00

abstract：:The carbohydrate binding properties of jacalin lectin were examined using RAF9 cell-derived D-[6-3H]glucosamine-radiolabeled total glycopeptides containing N-linked and O-linked oligosaccharides. The binding of N-linked glycopeptides to jacalin was abolished by treatment of alpha-galactosidase whereas O-linked glycope...

journal_title：FEBS letters

authors： Do SI,Lee KY

更新日期：1998-01-09 00:00:00

abstract：:To better understand the mechanism of intracellular Ca2+ mobilization, mouse oocytes were micro-injected with 'caged'-inositol-1,4,5 triphosphate caged-InsP3) together with the Ca2+ indicator Fluo-3 to directly induce and monitor Ca2+ redistribution. Photo-released InsP3 elicits [Ca2+]i changes exhibiting several kine...

journal_title：FEBS letters

authors： Peres A

更新日期：1990-11-26 00:00:00

abstract：:The alphavirus-like mRNA capping enzyme of Bamboo mosaic virus (BaMV) exhibits an AdoMet-dependent guanylyltransferase activity by which the methyl group of AdoMet is transferred to GTP, leading to the formation of m(7)GTP, and the m(7)GMP moiety is next transferred to the 5' end of ppRNA via a covalent enzyme-m(7)GMP...

journal_title：FEBS letters

authors： Lin HY,Yu CY,Hsu YH,Meng M

更新日期：2012-07-30 00:00:00

abstract：:A cDNA encoding a human liver UDPGT (HLUG 25) transcribed and translated in vitro showed that the encoded protein was synthesized as a precursor and was cleaved and glycosylated when dog pancreatic microsomes were present during translation. The UDPGT cDNA was transiently expressed in mammalian cell culture (COS-7 cel...

journal_title：FEBS letters

authors： Fournel-Gigleux S,Jackson MR,Wooster R,Burchell B

更新日期：1989-01-30 00:00:00

abstract：:The aim of this study was to evaluate, using a rat model of balloon angioplasty, whether prostaglandin (PG) J(2) and 2-cyclopenten-1-one are able to reduce restenosis. We found that both PGJ(2) and 2-cyclopenten-1-one, administered by local application on carotid arteries, caused a dose-dependent inhibition of neointi...

journal_title：FEBS letters

authors： Ianaro A,Maffia P,Cuzzocrea S,Mazzon E,Santoro MG,Di Rosa M,Ialenti A

更新日期：2003-10-09 00:00:00

abstract：:Cyanobacteria possess genes encoding phytochrome-related proteins. We used a DNA microarray approach to evaluate the impact of the phytochromes Cph1 and Cph2 on red light (R)- and far-red light (FR)-dependent gene expression in the unicellular cyanobacterium Synechocystis sp. PCC 6803. In cells of wild-type and phytoc...

journal_title：FEBS letters

authors： Hübschmann T,Yamamoto H,Gieler T,Murata N,Börner T

更新日期：2005-03-14 00:00:00

abstract：:Oligonucleotide-peptide conjugates have several applications, including their potential use as improved antisense agents for interfering with the RNA function within cells. In order to provide robust and generally applicable conjugation chemistry, we developed a novel approach of fragment coupling of pre-synthesized p...

journal_title：FEBS letters

authors： Zubin EM,Romanova EA,Volkov EM,Tashlitsky VN,Korshunova GA,Shabarova ZA,Oretskaya TS

更新日期：1999-07-30 00:00:00

abstract：:Platelet-activating factor (PAF) is a phospholipid, intermediate in its structure between diacyl- and lysophospholipids. The reorientation of this highly bioactive compound to the inner membrane layer of human erythrocytes after its primary incorporation into the outer membrane layer has been studied. Reorientation wa...

journal_title：FEBS letters

authors： Schneider E,Haest CW,Deuticke B

更新日期：1986-03-31 00:00:00

abstract：:Proteases not only play a fundamental role in numerous physiological processes, but are also involved in several human diseases including Alzheimer's disease (AD). A key protease implicated in AD is the so far unidentified gamma-secretase, which cleaves the membrane-bound beta-amyloid precursor protein (betaAPP) at th...

journal_title：FEBS letters

authors： Steiner H,Pesold B,Haass C

更新日期：1999-12-17 00:00:00

abstract：:Soluble aluminium (Al(3+)) is the major constraint to plant growth on acid soils. Plants have evolved mechanisms to tolerate Al(3+) and one type of mechanism relies on the efflux of organic anions that protect roots by chelating the Al(3+). Al(3+) resistance genes of several species have now been isolated and found to...

journal_title：FEBS letters

authors： Delhaize E,Gruber BD,Ryan PR

更新日期：2007-05-25 00:00:00

abstract：:Previously, we cloned rat MRP3 as a candidate for an inducible transporter for the biliary excretion of organic anions [Hirohashi et al. (1998) Mol. Pharmacol. 53, 1068-10751. In the present study, we cloned human MRP3 (1527 amino acids) from Caco-2 cells. Human MRP3 is predominantly expressed in liver, small intestin...

journal_title：FEBS letters

authors： Kiuchi Y,Suzuki H,Hirohashi T,Tyson CA,Sugiyama Y

更新日期：1998-08-14 00:00:00

abstract：:We have studied the endosome-lysosome transfer of internalized epidermal growth factor receptor (EGFR) complexes in a cell-free system from rat liver. Analytical subfractionation of a postmitochondrial supernatant fraction showed that a pulse of internalized [(125)I]EGF was largely associated with a light endosomal fr...

journal_title：FEBS letters

authors： Authier F,Chauvet G

更新日期：1999-11-12 00:00:00

abstract：:Resonance Raman spectroscopy was applied to monitor the effects of chloroquine (CQ) treatment on cultures of Plasmodium falciparum trophozoites. A number of bands assigned to A(1g) and B(1g) modes characteristic of the haemozoin aggregate are reduced in intensity in the CQ-treated cells, however, no bands from the CQ ...

journal_title：FEBS letters

authors： Webster GT,Tilley L,Deed S,McNaughton D,Wood BR

更新日期：2008-04-02 00:00:00

abstract：:The multidrug resistance protein (MRP) is an ATP-dependent transport protein for organic anions, as well as neutral or positively charged anticancer agents. In this study we report that dinitrophenyl-S-glutathione increases ATPase activity in plasma membrane vesicles prepared from the MRP-overexpressing cell line GLC4...

journal_title：FEBS letters

authors： Hooijberg JH,Broxterman HJ,Heijn M,Fles DL,Lankelma J,Pinedo HM

更新日期：1997-08-18 00:00:00

abstract：:Modulation of intracellular guanosine 3',5'-bispyrophosphate ((p)ppGpp) level, the effector of the stringent response, is crucial for survival as well as optimal growth of prokaryotes and, thus, for bacterial pathogenesis and dormancy. In Mycobacterium tuberculosis (Mtb), (p)ppGpp synthesis and degradation are carried...

journal_title：FEBS letters

authors： Singal B,Balakrishna AM,Nartey W,Manimekalai MSS,Jeyakanthan J,Grüber G

更新日期：2017-08-01 00:00:00

abstract：:Recombinant vaccinia virus vectors were used to coexpress each of the candidate prohormone convertases PC1, PC2, furin, PACE4 and PC5 with rat prosomatostatin (rProSOM) in the constitutive secreting cell line LoVo and in the endocrine corticotroph cell line AtT-20, which exhibits regulated secretion. Mammalian ProSOM ...

journal_title：FEBS letters

authors： Brakch N,Galanopoulou AS,Patel YC,Boileau G,Seidah NG

更新日期：1995-04-03 00:00:00

abstract：:Rhizobium sigma vegetative-dependent promoters are different from those of enteric bacteria and have never been characterized before. We report here the biochemical characterization of the ORF1-ntrBC promoter of Rhizobium etli. The minimal promoter region was located by means of a transcriptional fusion and further ch...

journal_title：FEBS letters

authors： Martino M,Riccio A,Defez R,Iaccarino M,Patriarca EJ

更新日期：1996-06-10 00:00:00

abstract：:Mitochondrial thioredoxin (mtTrx) can be oxidized in response to inducers of oxidative stress; yet the functional consequences of the oxidation have not been determined. This study evaluated the redox status of mtTrx and its association to oxidant-induced apoptosis. Results showed that mtTrx was oxidized after exposur...

journal_title：FEBS letters

authors： Chen Y,Cai J,Jones DP

更新日期：2006-12-11 00:00:00

abstract：:The green fluorescent protein YFP-H148Q is sensitive to halides by a mechanism involving halide binding and a shift in pK(a). However, a limitation of YFP-H148Q is its low halide sensitivity, with K(d)>100 mM for Cl(-). Indicators with improved sensitivities are needed for cell transport studies, particularly in drug ...

journal_title：FEBS letters

authors： Galietta LJ,Haggie PM,Verkman AS

更新日期：2001-06-22 00:00:00

abstract：:Chronic myelogenous leukemia (CML) is associated with the presence of the Philadelphia chromosome, which is generated by the reciprocal translocation of chromosomes 9 and 22. In the case of L6 (b2a2) mRNA, it is difficult to cleave the abnormal mRNA specifically because the mRNA includes no sequences that can be cleav...

journal_title：FEBS letters

authors： Hamada M,Kuwabara T,Warashina M,Nakayama A,Taira K

更新日期：1999-11-12 00:00:00

abstract：:The von Hippel-Lindau (VHL) tumor suppressor gene regulates the extracellular matrix by controlling fibronectin deposition. To identify novel VHL target genes, we subjected mRNA from VHL-deficient RCC cells (786-0-pRC) and a transfectant re-expressing wildtype VHL (786-0-VHL) to differential expression profiling. Amon...

journal_title：FEBS letters

authors： Bluyssen HA,Lolkema MP,van Beest M,Boone M,Snijckers CM,Los M,Gebbink MF,Braam B,Holstege FC,Giles RH,Voest EE

更新日期：2004-01-02 00:00:00

abstract：:A protein factor that specifically binds to the 5'-untranslated region of encephalomyocarditis virus (EMCV) RNA has been found in extracts of ascites carcinoma Krebs-2 cells. This was done using UV-irradiation on extracts supplemented with in vitro synthesized 32P-labelled transcripts followed by analysis of crosslink...

journal_title：FEBS letters

authors： Borovjagin AV,Evstafieva AG,Ugarova TYu,Shatsky IN

更新日期：1990-02-26 00:00:00

abstract：:During lactation glucose metabolism in paraovarian adipocytes is characterized by a 40 and 80% decrease of glucose incorporation into CO2 and fatty acids in the presence of insulin. In contrast with the stimulation by insulin of glucose incorporation into lactate, glycerol remains unchanged. As a result, insulin sensi...

journal_title：FEBS letters

authors： Burnol AF,Guerre-Millo M,Lavau M,Girard J

更新日期：1986-01-06 00:00:00