Abstract:
:The alphavirus-like mRNA capping enzyme of Bamboo mosaic virus (BaMV) exhibits an AdoMet-dependent guanylyltransferase activity by which the methyl group of AdoMet is transferred to GTP, leading to the formation of m(7)GTP, and the m(7)GMP moiety is next transferred to the 5' end of ppRNA via a covalent enzyme-m(7)GMP intermediate. The function of the conserved H68 of the BaMV capping enzyme in the intermediate formation was analyzed by mutagenesis in this study. The nature of the bond linking the enzyme and m(7)GMP was changed in the H68C mutant protein, strongly suggesting that H68 covalently binds to m(7)GMP in the intermediate.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Lin HY,Yu CY,Hsu YH,Meng Mdoi
10.1016/j.febslet.2012.05.024subject
Has Abstractpub_date
2012-07-30 00:00:00pages
2326-31issue
16eissn
0014-5793issn
1873-3468pii
S0014-5793(12)00399-7journal_volume
586pub_type
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