The liver-specific promoter of the human insulin-like growth factor-II gene contains two negative regulatory elements.

abstract：:The possible molecular heterogeneity of human transferrin receptors was analyzed using two murine monoclonal antibodies, Tü15 and Tü67. Both reagents precipitated from lysates of 125I-labeled HL-60 cells a major component of 88 kDa which could be identified as the transferrin receptor by comparison with the proteins d...

journal_title：FEBS letters

authors： Uchańska-Ziegler B,Wernet P,Liangru S,Ziegler A

更新日期：1984-10-01 00:00:00

abstract：:Osteogenesis depends on a coordinated network of transcription factors including Sp7. Emerging evidence indicates that microRNAs (miRNAs) act as pivotal regulators in various biological processes including osteoblast proliferation and differentiation. Here, we investigated the effect of miR-145 on osteogenic different...

journal_title：FEBS letters

authors： Jia J,Tian Q,Ling S,Liu Y,Yang S,Shao Z

更新日期：2013-09-17 00:00:00

abstract：:Chloride intracellular channel proteins (CLICs) are distinct from most ion channels in that they have both soluble and integral membrane forms. CLICs are highly conserved in chordates, with six vertebrate paralogues. CLIC-like proteins are found in other metazoans. CLICs form channels in artificial bilayers in a proce...

journal_title：FEBS letters

authors： Littler DR,Harrop SJ,Goodchild SC,Phang JM,Mynott AV,Jiang L,Valenzuela SM,Mazzanti M,Brown LJ,Breit SN,Curmi PM

更新日期：2010-05-17 00:00:00

abstract：:The regulation by insulin and carbohydrates of the gene expression of three key enzymes involved in glucose metabolism was studied in the liver of the Wistar fatty rat, a model of obese non-insulin-dependent diabetes mellitus. A high glucose or fructose diet, or insulin administration caused a similar magnitude of inc...

journal_title：FEBS letters

authors： Noguchi T,Matsuda T,Tomari Y,Yamada K,Imai E,Wang Z,Ikeda H,Tanaka T

更新日期：1993-08-09 00:00:00

abstract：:IRF-8/ICSBP and IRF-1 are IRF family members whose expression is induced in response to IFN-gamma in macrophages. IL-12 is a cytokine produced in macrophages that plays a critical role in host defense. IFN-gamma and bacterial lipopolysaccharide (LPS) induce IL-12p40 transcription, which is necessary for the production...

journal_title：FEBS letters

authors： Masumi A,Tamaoki S,Wang IM,Ozato K,Komuro K

更新日期：2002-11-06 00:00:00

abstract：:Canine aortic strip studies revealed insensitivity of angiotensin II (AII)-induced aortic contraction to inhibition by the non-peptide antagonist DuP753 (pKB = 6.7 +/- 0.1). In order to determine the origin of this phenomenon we cloned the canine homologue of the AT1 AII receptor. Expression of this cDNA in COS-7 cell...

journal_title：FEBS letters

authors： Burns L,Clark KL,Bradley J,Robertson MJ,Clark AJ

更新日期：1994-04-25 00:00:00

abstract：:Fas ligand (FasL) binds Fas (CD95) to induce apoptosis or activate other signaling pathways. In addition, FasL transduces bidirectional or 'reverse signals'. The intracellular domain of FasL contains consensus sequences for phosphorylation and an extended proline rich region, which regulate its surface expression thro...

journal_title：FEBS letters

authors： Thornhill PB,Cohn JB,Drury G,Stanford WL,Bernstein A,Desbarats J

更新日期：2007-09-18 00:00:00

abstract：:Hydrin 2 (vasotocinyl-Gly), a hydroosmotic peptide resulting from differential processing of provasotocin and recently identified in frog neurohypophysis, has been looked for in the pituitary gland of an exotic toad (Bufo marinus) and of a reptile (Vipera aspis). Hydrin 2 has been found in the amphibian but not in the...

journal_title：FEBS letters

authors： Michel G,Rouillé Y,Chauvet MT,Chauvet J,Acher R

更新日期：1990-05-07 00:00:00

abstract：:The 23-residue synthetic peptide representing the N-terminus of HIV-1 gp41 is known to induce either leakage or fusion of lipid vesicles depending on the experimental conditions. In this paper we report that a polar amino acid substitution V-->E at position 2, known to block gp41 activity in vivo, makes the peptide un...

journal_title：FEBS letters

authors： Pereira FB,Goñi FM,Nieva JL

更新日期：1995-04-03 00:00:00

abstract：:Hydrogenase gene from Clostridium butyricum was cloned in Escherichia coli HK16 (Hyd-) using pBR322 and PstI. The plasmid, pCBH1, containing hydrogenase gene was 7.3 MDa and pCBH1 had 5 PstI-DNA fragments (3.9, 2.6, 0.7, 0.03-0.04, less than 0.02 MDa, respectively). The hydrogenase activity of HK16 (pCBH1) was about 3...

journal_title：FEBS letters

authors： Karube I,Urano N,Yamada T,Hirochika H,Sakaguchi K

更新日期：1983-07-11 00:00:00

abstract：:MRI has the ability to generate high-contrast and high-resolution images, to obtain multiple diagnostic evaluations of organ function and morphology, and to provide multiple image planes with no risk of ionizing radiation. Recent efforts have focused on using MR technology to monitor gene delivery, to enhance gene tra...

journal_title：FEBS letters

authors： Yang X,Atalar E

更新日期：2006-05-22 00:00:00

abstract：:Hypo-osmotic swelling of human Intestine 407 cells leads to a significant increase of intracellular MAPKAP-kinase 2 activity and Hsp27 phosphorylation. Pre-treatment of the cells with the p38 MAP kinase inhibitor SB-203580 blocks this activation, indicating that the hypotonicity-induced activation of MAPKAP kinase 2 i...

journal_title：FEBS letters

authors： Tilly BC,Gaestel M,Engel K,Edixhoven MJ,de Jonge HR

更新日期：1996-10-21 00:00:00

abstract：:1H-NMR spectroscopy has been applied to identify components in the urine of subjects with a deficiency of the enzyme 3-hydroxy-3-methylglutaryl-CoA lyase. One-dimensional spectra of samples from a pair of non-identical twins with this disorder were very similar and are probably diagnostic. The most intense signals wer...

journal_title：FEBS letters

authors： Iles RA,Jago JR,Williams SR,Chalmers RA

更新日期：1986-07-14 00:00:00

abstract：:We have expressed green fluorescent protein (GFP) from A. victoria in the secretory pathway of HeLa cells by fusing it to the C-terminus of a secretory protein, chromogranin B. Under normal culture conditions at 37 degrees C maturation of GFP to the fluorescent form was not detectable. However, fluorescent GFP was obs...

journal_title：FEBS letters

authors： Kaether C,Gerdes HH

更新日期：1995-08-07 00:00:00

abstract：:α-Helical membrane-active antimicrobial peptides (AMPs) are known to act via a range of mechanisms, including the formation of barrel-stave and toroidal pores and the micellisation of the membrane (carpet mechanism). Different mechanisms imply that the peptides adopt different 3D structures when bound at the water-mem...

journal_title：FEBS letters

authors： van den Bergen G,Stroet M,Caron B,Poger D,Mark AE

更新日期：2020-03-01 00:00:00

abstract：:In vertebrate photoreceptors the soluble protein arrestin (45 kDa) is involved in controlling the light dependent activity of receptor proteins such as transducin or the cGMP-phosphodiesterase. Arrestin has further been identified as the retinal-S-antigen which is assumed to cause the autoimmune disease uveitis. In a ...

journal_title：FEBS letters

authors： Glitscher W,Rüppel H

更新日期：1991-05-06 00:00:00

abstract：:The ability of three fatty acids and their respective ethyl esters, to promote generation of reactive oxygen species (ROS), was compared in a preparation of rat brain synaptosomes. Arachidonic but not palmitic or linoleic acids promoted ROS generation. Ethyl esterification of each fatty acid significantly enhanced ROS...

journal_title：FEBS letters

authors： Bondy SC,Marwah S

更新日期：1995-11-13 00:00:00

abstract：:Yeast cells lacking the metacaspase-encoding gene YCA1 (Deltayca1) were compared with wild-type (WT) cells with respect to the occurrence, nature and time course of acetic-acid triggered death. We show that Deltayca1 cells undergo programmed cell death (PCD) with a rate lower than that of the WT and that PCD in WT cel...

journal_title：FEBS letters

authors： Guaragnella N,Pereira C,Sousa MJ,Antonacci L,Passarella S,Côrte-Real M,Marra E,Giannattasio S

更新日期：2006-12-22 00:00:00

abstract：:Acute hyperammonemia, induced by two consecutive injections of ammonium acetate (550 and 450 mg per kg b.wt.), decreased the activity of gamma-glutamyl transpeptidase (GGT) in most brain regions of 18- and 30-day-old rats. This decrease in the brain GGT activity was more pronounced in younger than in older rats. After...

journal_title：FEBS letters

authors： Stastný F,Pitha J,Lisý V,Hilgier W,Kaucká I,Albrecht J

更新日期：1992-04-06 00:00:00

abstract：:Crystals of methylamine-treated alpha 2-macroglobulin (alpha 2M-MA), alpha 2-macroglobulin in complex with two molecules of trypsin, alpha 2M-T2, one molecule of plasmin, alpha 2M-PL, and one molecule of plasmin followed by methylamine-treatment, alpha 2M-PL(MA), have reproducibly been obtained using ammonium sulfate ...

journal_title：FEBS letters

authors： Andersen GR,Jacobsen L,Thirup S,Nyborg J,Sottrup-Jensen L

更新日期：1991-11-04 00:00:00

abstract：:We reported the preparation of a novel trehalose derivative based on enzymatic oxidation of trehalose by water-soluble glucose-3-dehydrogenase (G3DH) from marine bacterium Halomonas sp. alpha-15 cells. The product of G3DH enzymatic conversion was 3,3'-diketotrehalose (3,3'dkT), a novel trehalose derivative of which bo...

journal_title：FEBS letters

authors： Sode K,Akaike E,Sugiura H,Tsugawa W

更新日期：2001-01-26 00:00:00

abstract：:Electron paramagnetic resonance (EPR) spectroscopy and analysis of the primary structure of the CMP-N-acetylneuraminic acid hydroxylase revealed that this enzyme is the first iron-sulphur protein of the Rieske type to be found in the cytosol of Eukarya. The dithionite-reduced hydroxylase exhibited an EPR signal known ...

journal_title：FEBS letters

authors： Schlenzka W,Shaw L,Kelm S,Schmidt CL,Bill E,Trautwein AX,Lottspeich F,Schauer R

更新日期：1996-05-06 00:00:00

abstract：:The interaction of Bordetella pertussis adenylate cyclase toxin (CyaA) with complement receptor 3 (CR3, CD11b/CD18) involves N-linked oligosaccharide chains. To investigate the relative importance of the individual N-glycans of CR3 for toxin activity, the asparagine residues of the consensus N-glycosylation sites of C...

journal_title：FEBS letters

authors： Hasan S,Osickova A,Bumba L,Novák P,Sebo P,Osicka R

更新日期：2015-01-30 00:00:00

abstract：:Using a monoclonal antibody as conformational probe it has been shown that the weakly active nicked-beta 2 dimer of tryptophan synthase generated by proteolytic cleavage at Glu-296, does not undergo on association with alpha subunit a conformational change known to occur in intact beta 2 subunit. This alpha induced co...

journal_title：FEBS letters

authors： Linkens HJ,Djavadi-Ohaniance L,Goldberg ME

更新日期：1993-04-12 00:00:00

abstract：:Chromogranin A is known to undergo pH induced conformational changes, and the difference in conformation is supposed to be responsible for the difference in Ca2+ binding property. To gain insight regarding the overall structure and the nature of pH-induced conformational changes of chromogranin A, limited trypsin dige...

journal_title：FEBS letters

authors： Yoo SH,Ferretti JA

更新日期：1993-11-22 00:00:00

abstract：:Osteoblasts and adipocytes are believed to share a common progenitor. Peroxisome proliferator-activated receptor gamma (PPARgamma) plays a key role in the switching of these two cell lineages. Here, we demonstrated the differentiation of ES cells into an osteoblastic lineage using siRNA against PPARgamma without the a...

journal_title：FEBS letters

authors： Yamashita A,Takada T,Nemoto K,Yamamoto G,Torii R

更新日期：2006-07-24 00:00:00

abstract：:A new enzyme catalyzing the deamidation of seed storage proteins was found in germinating wheat grains and was partially purified. It also acts on egg lysozyme, horse hemoglobin and reduced RNAse, glutamine and Gly-L-Gln-L-Tyr. No activity was observed when using ovalbumin, serum albumin, RNAse, insulin, asparagine an...

journal_title：FEBS letters

authors： Vaintraub IA,Kotova LV,Shaha R

更新日期：1992-05-11 00:00:00

abstract：:The interferon inducible transmembrane (IFITM) proteins mediate several cellular processes such as homotypic cell adhesion functions of interferons (IFNs) and cellular anti-proliferative activities. We show that the BAF complex-mediated induction of IFITM3 is dependent on binding of the transcriptional enhancer factor...

journal_title：FEBS letters

authors： Cuddapah S,Cui K,Zhao K

更新日期：2008-01-23 00:00:00

abstract：:A steady-state framework was applied to the ubiquitous tricyclic enzyme cascade structure, as seen in the mitogen-activated protein (MAP) kinase system, to analyze the effect of upstream kinase concentrations on final output response. The results suggest that signal amplification achieved by the cascade structure ensu...

journal_title：FEBS letters

authors： Mutalik VK,Singh AP,Edwards JS,Venkatesh KV

更新日期：2004-01-30 00:00:00

abstract：:C6 rat glioma cells persistently infected with subacute sclerosing panencephalitis virus (C6/SSPE) were treated with measles antiserum and purified anti-measles IgG. This stimulated phosphoinositide breakdown and an increase in inositol phosphates. In uninfected C6 cells, however, only fetal calf serum (FCS), but not ...

journal_title：FEBS letters

authors： Weinmann-Dorsch C,Koschel K

更新日期：1989-04-24 00:00:00