Abstract:
:Canine aortic strip studies revealed insensitivity of angiotensin II (AII)-induced aortic contraction to inhibition by the non-peptide antagonist DuP753 (pKB = 6.7 +/- 0.1). In order to determine the origin of this phenomenon we cloned the canine homologue of the AT1 AII receptor. Expression of this cDNA in COS-7 cells indicated a low affinity of DuP753 for the cloned receptor (KD = 92 nM). The predicted amino acid sequence is highly homologous to other mammalian AT1 receptors; sequence comparisons suggest the pharmacological difference may be the result of a threonine residue in position 163 in the IVth transmembrane domain.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Burns L,Clark KL,Bradley J,Robertson MJ,Clark AJdoi
10.1016/0014-5793(94)80307-2subject
Has Abstractpub_date
1994-04-25 00:00:00pages
146-50issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(94)80307-2journal_volume
343pub_type
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