Effect of perturbing diacylglycerol metabolism on cytosolic free Ca2+ oscillations induced in single hepatocytes.

abstract：:Interfering RNA was used to suppress the expression of the genes At1g06680 and At2g30790 in Arabidopsis thaliana, which encode the PsbP-1 and PsbP-2 proteins, respectively, of Photosystem II. A phenotypic series of transgenic plants was recovered that expressed intermediate and low amounts of PsbP. Earlier we had docu...

journal_title：FEBS letters

authors： Yi X,Hargett SR,Frankel LK,Bricker TM

更新日期：2009-06-18 00:00:00

abstract：:To understand the role of nitric oxide (NO) in controlling the specific DNA-binding activities of transcriptional factors, we investigated the in vitro effect of the NO-donor sodium nitroprusside (SNP) on the AP-1 activity of cultured mouse cerebellar granule cells. A gel-mobility assay showed that SNP inhibited AP-1 ...

journal_title：FEBS letters

authors： Tabuchi A,Sano K,Oh E,Tsuchiya T,Tsuda M

更新日期：1994-08-29 00:00:00

abstract：:Recent studies show LDL receptor-related protein 1B, LRP1B as a transducer of extracellular signals. Here, we identify six interacting partners of the LRP1B cytoplasmic region by yeast two-hybrid screen and confirmed their in vivo binding by immunoprecipitation. One of the partners, PICK1 recognizes the C-terminus of ...

journal_title：FEBS letters

authors： Shiroshima T,Oka C,Kawaichi M

更新日期：2009-01-05 00:00:00

abstract：:DNA-cytochrome c complexes adsorbed on carbon-coated mica surfaces were directly imaged by atomic force microscopy in air using commercially available cantilevers, with a routine resolution of 6 nm. Images of M13 phage DNA and M13-DNA polymerase complex are also shown. ...

journal_title：FEBS letters

authors： Yang J,Takeyasu K,Shao Z

更新日期：1992-04-20 00:00:00

abstract：:When cultured porcine aortic endothelial cells (ECs) were incubated with porcine big endothelin-1 (bit ET-1(1-39)), there was a time-dependent increase in immunoreactive (IR)-ET in the culture supernatant, in addition to an endogenous IR-ET release fron the cells. Reverse-phase HPLC of the culture supernatant revealed...

journal_title：FEBS letters

authors： Ikegawa R,Matsumura Y,Tsukahara Y,Takaoka M,Morimoto S

更新日期：1991-11-18 00:00:00

abstract：:The photophobic receptor (psRII) and its transducer pHtrII from Natronobacterium pharaonis were heterologously coexpressed in the phototaxis-deficient Halobacterium salinarium strain Pho81/w which lacks all four bacterial rhodopsins, i.e. the two ion pumps bacteriorhodopsin and halorhodopsin as well as the two sensory...

journal_title：FEBS letters

authors： Lüttenberg B,Wolff EK,Engelhard M

更新日期：1998-04-10 00:00:00

abstract：:To investigate the cis-acting DNA elements that are involved in the regulation of class I major histocompatibility complex genes by interferon, several promoter fragments of the H-2Kk gene were linked to the reporter chloramphenicol acetyl transferase (CAT) gene, and the CAT expression was analyzed in stable transfect...

journal_title：FEBS letters

authors： Pascucci A,Pannuti A,La Mantia G,Lania L

更新日期：1988-01-04 00:00:00

abstract：:Xenopus oocytes expressing neuronal alpha 1C, alpha 2 and beta 1b calcium channel subunit cDNAs were used in this study. During two-electric voltage clamp recording the oocyte was injected with 10-20 nl of a 100 mM BAPTA solution. Under these conditions, the endogenous Ca-activated Cl current was completely suppressed...

journal_title：FEBS letters

authors： Charnet P,Bourinet E,Dubel SJ,Snutch TP,Nargeot J

更新日期：1994-05-09 00:00:00

abstract：:We show that the calcium-mimic dye, Stains-all, is a convenient probe to study the structural features of the individual calcium-binding sites of calmodulin (CaM) and related calcium-binding proteins (CaBP). These peptides bind the dye in their calcium-binding sites, and induce a circular dichroism (CD) band in the bo...

journal_title：FEBS letters

authors： Sharma Y,Gopalakrishna A,Balasubramanian D,Fairwell T,Krishna G

更新日期：1993-07-12 00:00:00

abstract：:Adenosine and ATP modulate cellular and tissue functions via specific P1 and P2 receptors, respectively. Although, in general, adenosine inhibits excitability and ATP functions as an excitatory transmitter in the central nervous system, little is known about the direct interaction between P1 and P2 receptors. We recen...

journal_title：FEBS letters

authors： Yoshioka K,Hosoda R,Kuroda Y,Nakata H

更新日期：2002-11-06 00:00:00

abstract：:Azide-binding to the heme-copper binuclear center of bo-type ubiquinol oxidase from Escherichia coli was investigated with Fourier-transform infrared spectroscopy. Deconvolution analyses of infrared spectra of the azide (14N3)-inhibited air-oxidized form showed a major infrared azide antisymmetric stretching band at 2...

journal_title：FEBS letters

authors： Tsubaki M,Mogi T,Hori H

更新日期：1999-04-23 00:00:00

abstract：:HGTD-P is a hypoxia-responsive pro-apoptotic protein that transmits hypoxic signals directly to mitochondria. When overexpressed, HGTD-P induces cell death via typical mitochondrial apoptotic cascades. However, much is unknown about post-transcriptional modification and signaling networks of HGTD-P in association with...

journal_title：FEBS letters

authors： Kim JY,Kim SM,Ko JH,Yim JH,Park JH,Park JH

更新日期：2006-05-29 00:00:00

abstract：:Chicken muscular dystrophy with abnormal muscle (AM) has been studied for more than 50 years, but the gene responsible for it remains unclear. Our previous studies narrowed down the AM candidate region to approximately 1Mbp of chicken chromosome 2q containing seven genes. In this study, we performed sequence compariso...

journal_title：FEBS letters

authors： Matsumoto H,Maruse H,Inaba Y,Yoshizawa K,Sasazaki S,Fujiwara A,Nishibori M,Nakamura A,Takeda S,Ichihara N,Kikuchi T,Mukai F,Mannen H

更新日期：2008-06-25 00:00:00

abstract：:Prokaryotes, plants and animals control ammonium fluxes by the regulated expression of ammonium transporters (AMTs) and/or the related Rhesus (Rh) proteins. Plant AMTs were previously reported to mediate electrogenic transport. Functional analysis of AtAMT2 from Arabidopsis in yeast and oocytes suggests that NH(4)(+) ...

journal_title：FEBS letters

authors： Neuhäuser B,Dynowski M,Ludewig U

更新日期：2009-09-03 00:00:00

abstract：:We counted base transitions (Ts) and transversions (Tv) in mutations among various types of hepatitis C virus (HCV) sequences. The Ts/Tv ratio was useful for distinguishing the (geno)type of the compared HCV sequences, since the ratio was 4.0 or more among sequences of the same type, while it was less than 2.0 among s...

journal_title：FEBS letters

authors： Tanaka T,Kato N,Hijikata M,Shimotohno K

更新日期：1993-01-04 00:00:00

abstract：:Transthyretin (TTR) is a circulatory protein which plays an important role in the transport of both thyroid hormone and retinol. Hep G2 cells, a human hepatoma-derived cell line, have been used extensively in studies of protein secretion by liver cells. The original description of this cell line indicated that this li...

journal_title：FEBS letters

authors： Blaner WS,Bonifácio MJ,Feldman HD,Piantedosi R,Saraiva MJ

更新日期：1991-08-05 00:00:00

abstract：:Phospholamban (PLB), an integral membrane protein of cardiac sarcoplasmic reticulum (SR), is described as the regulator of the Ca(2+)-ATPase pump, via its phosphorylation-dephosphorylation of Ser-16. Recently it has been shown that a direct interaction between the N-terminal hydrophilic domain of PLB and Ca(2+)-ATPase...

journal_title：FEBS letters

authors： Terzi E,Poteur L,Trifilieff E

更新日期：1992-09-14 00:00:00

abstract：:FACS analysis together with PIPLC treatment was applied to PI-anchoring antigens such as DAF (decay-accerelating factor, CD55), 1F5 antigen (CD59), CD14 and CD16 on the cell surfaces of blood cells from a normal adult and a male patient with paroxysmal nocturnal hemoglubinuria (PNH). Through the extensive analysis, th...

journal_title：FEBS letters

authors： Taguchi R,Funahashi Y,Ikezawa H,Nakashima I

更新日期：1990-02-12 00:00:00

abstract：:Cap-independent translation (CIT) occurs at the leader sequences of uncapped plant viral RNAs, but also at a number of normally capped cellular mRNAs and has been correlated with sequence complementarity to 18S rRNA. The ribosomal protein S18 (RPS18) is a component of the small ribosomal subunit and is encoded by thre...

journal_title：FEBS letters

authors： Vanderhaeghen R,De Clercq R,Karimi M,Van Montagu M,Hilson P,Van Lijsebettens M

更新日期：2006-05-15 00:00:00

abstract：:Glucocorticoid hormones (GHs) regulate cell proliferation of neural progenitor cells (NPCs) contributing to reduction of neurogenesis after stress. We show here that dexamethasone (Dex) decreases BRUCE/Apollon (BRUCE) in cultured NPCs in a GH-receptor-dependent manner. Downregulation of BRUCE by Dex or using silencing...

journal_title：FEBS letters

authors： Sippel M,Rajala R,Korhonen L,Bornhauser B,Sokka AL,Naito M,Lindholm D

更新日期：2009-07-07 00:00:00

abstract：:The OxyR protein is a transcriptional activator for a subset of peroxide stress-inducible genes, most of which are involved in defense systems against oxidative stress. Recently, it was demonstrated that purified OxyR has one intramolecular disulfide bond, which led to the proposal that the reversible disulfide bond f...

journal_title：FEBS letters

authors： Tao K

更新日期：1999-08-20 00:00:00

abstract：:The maturation of Borna disease virus (BDV) glycoprotein GP was studied in regard to intracellular compartmentalization, compartmentalization signal-domains, proteolytic processing, and packaging into virus particles. Our data show that BDV-GP is (i) predominantly located in the endoplasmic reticulum (ER), (ii) partia...

journal_title：FEBS letters

authors： Eickmann M,Kiermayer S,Kraus I,Gössl M,Richt JA,Garten W

更新日期：2005-08-29 00:00:00

abstract：:Hordeum vulgare L. yellow stripe 1 (HvYS1) is a selective transporter for Fe(III)-phytosiderophores, involved in primary iron acquisition from soils in barley roots. In contrast, Zea mays yellow stripe 1 (ZmYS1) in maize possesses broad substrate specificity, despite a high homology with HvYS1. Here we revealed, by as...

journal_title：FEBS letters

authors： Harada E,Sugase K,Namba K,Iwashita T,Murata Y

更新日期：2007-09-04 00:00:00

abstract：:FT-IR analysis shows that treatment of F1ATPase with the inhibitors DCCD and Nbf-Cl, in the presence of saturating concentrations of ADP and AMP-PNP and in the absence of Mg2+, does not modify the secondary structure of the enzyme, but significantly modifies its compactness and thermal stability, although to different...

journal_title：FEBS letters

authors： Lippe G,Tanfani F,Di Pancrazio F,Contessi S,Bertoli E,Dabbeni-Sala F

更新日期：1998-08-07 00:00:00

abstract：:Macrophages are abundant in the tumor microenvironment. They are highly plastic and able to acquire pro-tumoral phenotypes in response to microenvironmental stimuli. When we treated RAW 264.7 macrophages with inhibitors of various oncogenic pathways, we found that the focal adhesion kinase (FAK) inhibitors PF573228 an...

journal_title：FEBS letters

authors： He X,Chen X,Li B,Ji J,Chen S

更新日期：2017-12-01 00:00:00

abstract：:New polypeptide components have been isolated from Dendroaspis angusticeps venom using chromatography. Two polypeptides containing 59 and 57 amino acids, called 'DaE1' and 'DaE2' respectively, have been purified to homogeneity and fully sequenced. Spectrometric analysis yielded masses of 6631.5 and 6389.0 Da, respecti...

journal_title：FEBS letters

authors： Tytgat J,Vandenberghe I,Ulens C,Van Beeumen J

更新日期：2001-03-02 00:00:00

abstract：:Present analysis procedures for NMR structure determination of macromolecules presuppose fixed internuclear distances. Improvement of the precision of the requisite NOE information has stimulated the use of more quantitative distance constraints thus necessitating examination as to whether the assumption of a rigid mo...

journal_title：FEBS letters

authors： LeMaster DM,Kay LE,Brünger AT,Prestegard JH

更新日期：1988-08-15 00:00:00

abstract：:Calf thymus (ct) Hsc70 has been shown previously to reactivate heat-inactivated prokaryotic and eukaryotic enzymes, while DnaK was able to reactivate solely prokaryotic enzymes. Here, we report on isolation from calf thymus of a DnaJ homolog, ctHsc40, and on testing of its cooperative function in three different assay...

journal_title：FEBS letters

authors： Ziemienowicz A,Konieczny I,Hübscher U

更新日期：2001-10-19 00:00:00

abstract：:The eukaryotic transcription factor NF-kappaB is activated by a large variety of stimuli. We have recently shown that ER stress, caused by an aberrant accumulation of membrane proteins within this organelle, also activates NF-kappaB. Here, we show that activation of NF-kappaB by ER stress requires an increase in the i...

journal_title：FEBS letters

authors： Pahl HL,Baeuerle PA

更新日期：1996-08-26 00:00:00

abstract：:Although bird species studied thus far have no distinct brown adipose tissue (BAT) or a related thermogenic tissue, there is now strong evidence that non-shivering mechanisms in birds may play an important role during cold exposure. Recently, increased expression of the duckling homolog of the avian uncoupling protein...

journal_title：FEBS letters

authors： Toyomizu M,Ueda M,Sato S,Seki Y,Sato K,Akiba Y

更新日期：2002-10-09 00:00:00