Light-dependent delta mu Na-generation and utilization in the marine cyanobacterium Oscillatoria brevis.

abstract：:TP53 belongs to a small gene family that includes, in mammals, two additional paralogs, TP63 and TP73. The p63 and p73 proteins are structurally and functionally similar to p53 and their activity as transcription factors is regulated by a wide repertoire of shared and unique post-translational modifications and intera...

journal_title：FEBS letters

authors： Costanzo A,Pediconi N,Narcisi A,Guerrieri F,Belloni L,Fausti F,Botti E,Levrero M

更新日期：2014-08-19 00:00:00

abstract：:The complete amino acid sequence of the oligomycin sensitivity-conferring protein (OSCP) of beef-heart mitochondria is reported. The protein contains 190 amino acids and has a molecular mass of 20 967. Its structure is characterized by a concentration of charged amino acids in the two terminal segments (N 1-77 and C 1...

journal_title：FEBS letters

authors： Ovchinnikov YA,Modyanov NN,Grinkevich VA,Aldanova NA,Trubetskaya OE,Nazimov IV,Hundal T,Ernster L

更新日期：1984-01-23 00:00:00

abstract：:The conformation of the glycine-rich loop of adenylate kinase is described in detail. It forms a giant anion hole for a sulfate ion, which presumably mimicks a nucleotide phosphoryl group. This loop had been called flexible, because at pH values of 6 or below it is displaced in the crystal. In the region of this loop ...

journal_title：FEBS letters

authors： Dreusicke D,Schulz GE

更新日期：1986-11-24 00:00:00

abstract：:The WWE domain is often identified in proteins associated with ubiquitination or poly-ADP-ribosylation. Structural information about WWE domains has been obtained for the ubiquitination-related proteins, such as Deltex and RNF146, but not yet for the poly-ADP-ribose polymerases (PARPs). Here we determined the solution...

journal_title：FEBS letters

authors： He F,Tsuda K,Takahashi M,Kuwasako K,Terada T,Shirouzu M,Watanabe S,Kigawa T,Kobayashi N,Güntert P,Yokoyama S,Muto Y

更新日期：2012-11-02 00:00:00

abstract：:Researchers still have great difficulty in isolating individual ribosomal proteins from the ribosome in quantities high enough for structural research. To this end, when studying protein S7, we created an E. coli overproducer of the recombinant protein S7 of Thermus thermophilus. The vector for expression was pQE-32 h...

journal_title：FEBS letters

authors： Karginov AV,Karginova OA,Spiridonova VA,Kopylov AM

更新日期：1995-08-07 00:00:00

abstract：:We have investigated the possible role of selected negatively-charged amino acids of the sodium and chloride-coupled GABA transporter GAT-1 on sodium binding. These residues located adjacent to putative transmembrane domains and which are conserved throughout the large superfamily of neurotransmitter transporters were...

journal_title：FEBS letters

authors： Keshet GI,Bendahan A,Su H,Mager S,Lester HA,Kanner BI

更新日期：1995-08-28 00:00:00

abstract：:C26, the C-terminal 26 residue peptide of serpin A1, significantly increased cell proliferation in cultures of hepatoma cells, but not in porcine kidney epithelial cells, human skin fibroblasts or keratinocytes. The mitogenic activity of C26 was preferentially inhibited with a protein kinase C (PKC) inhibitor, an anti...

journal_title：FEBS letters

authors： Congote LF,Temmel N

更新日期：2004-10-22 00:00:00

abstract：:The importance of the individual amino acid residues of AIP (KKALRRQEAVDAL), a highly specific inhibitor of calmodulin-dependent protein kinase II (CaMKII), was studied. Replacement of Arg6, Gln7, or Ala9 by other amino acid residues produced a marked increase in the IC50 value. Leu4 and Val10 were also sensitive to r...

journal_title：FEBS letters

authors： Ishida A,Shigeri Y,Tatsu Y,Uegaki K,Kameshita I,Okuno S,Kitani T,Yumoto N,Fujisawa H

更新日期：1998-05-01 00:00:00

abstract：:Thrombin has been shown to inhibit skeletal muscle differentiation. However, the mechanisms by which thrombin represses myogenesis remain unknown. Since the thrombin receptor couples to G(i), G(q/11) and G(12), we examined which subunits of heterotrimeric guanine nucleotide-binding regulatory proteins (Galpha(i), Galp...

journal_title：FEBS letters

authors： Nagao M,Kaziro Y,Itoh H

更新日期：2000-04-28 00:00:00

abstract：:A possible interaction between tumor necrosis factor-alpha (TNF) and other cytokines/growth factors in stimulating the production of nerve growth factor (NGF) in Swiss 3T3 cells was studied. TNF's stimulatory activity on fibroblast NGF production was synergized by interleukin-1 alpha (IL-1 alpha), IL-1 beta and interf...

journal_title：FEBS letters

authors： Hattori A,Iwasaki S,Murase K,Tsujimoto M,Sato M,Hayashi K,Kohno M

更新日期：1994-03-07 00:00:00

abstract：:The activity of the Ca-pumping ATPase of cardiac sarcoplasmic reticulum (SR) is controlled by the phosphorylation of the intrinsic regulatory protein phospholamban (PLB), which affects both the apparent Km(Ca) and the Vmax of the transport process. We have investigated the correlation between phosphorylation of PLB an...

journal_title：FEBS letters

authors： Chiesi M,Schwaller R

更新日期：1989-02-13 00:00:00

abstract：:PsbV2 is a c-type cytochrome present in a very low abundance in the thermophilic cyanobacterium Thermosynechococcus elongatus. We purified this cytochrome and solved its crystal structure at a resolution of 1.5Å. The protein existed as a dimer in the crystal, and has an overall structure similar to other c-type cytoch...

journal_title：FEBS letters

authors： Suga M,Lai TL,Sugiura M,Shen JR,Boussac A

更新日期：2013-10-01 00:00:00

abstract：:The anti-ICAM-1 monoclonal antibody F10.2 was conjugated to liposomes to target to cells expressing the cell adhesion molecule ICAM-1. We demonstrate that F10.2 immunoliposomes bind to human bronchial epithelial cells (BEAS-2B) and human umbilical vein endothelial cells (HUVEC) in a specific, dose- and time-dependent ...

journal_title：FEBS letters

authors： Bloemen PG,Henricks PA,van Bloois L,van den Tweel MC,Bloem AC,Nijkamp FP,Crommelin DJ,Storm G

更新日期：1995-01-03 00:00:00

abstract：:Neutrophils contain four uncharacterized protein kinases with molecular masses of ca. 69, 63, 49 and 40 kDa that are rapidly activated upon stimulation of these cells with the chemoattractant fMet-Leu-Phe [Ding, J. and Badwey, J.A. (1993) J. Biol. Chem. 268, 17326-17333]. We now report that wortmannin and 1-butanol bl...

journal_title：FEBS letters

authors： Ding J,Badwey JA

更新日期：1994-07-11 00:00:00

abstract：:The membrane-membrane fusion during fertilization of oocyte by spermatozoa is believed to be mainly mediated by so called "fusion proteins". In the present study we have tried to demonstrate that beside the proteins, lipid components of membrane may play an important role in fusion of oocyte with spermatozoa. Conventi...

journal_title：FEBS letters

authors： Atif SM,Hasan I,Ahmad N,Khan U,Owais M

更新日期：2006-04-17 00:00:00

abstract：:Histone deacetylase 6 (HDAC6) is a multi-substrate cytoplasmic enzyme that regulates many important biological processes. Recently, some reports have implicated HDAC6 in viral infection. However, nothing is known about its regulation in virus-infected cells. The data presented here for the first time demonstrate the c...

journal_title：FEBS letters

authors： Husain M,Harrod KS

更新日期：2009-08-06 00:00:00

abstract：:In plant organelle genomes, homeologous recombination between heteroallelic positions of repetitive sequences is increased by dysfunction of the gene encoding MutS homolog 1 (MSH1), a plant organelle-specific homolog of bacterial mismatch-binding protein MutS1. The C-terminal region of plant MSH1 contains the GIY-YIG ...

journal_title：FEBS letters

authors： Fukui K,Harada A,Wakamatsu T,Minobe A,Ohshita K,Ashiuchi M,Yano T

更新日期：2018-12-01 00:00:00

abstract：:Heparin-binding epidermal growth factor-like growth factor (HB-EGF), a potent mitogen and migration factor for vascular smooth muscle cells (SMC), promoted neovascularization in vivo in the rabbit cornea. MRI demonstrated quantitatively the angiogenic effect of HB-EGF when introduced subcutaneously into nude mice. HB-...

journal_title：FEBS letters

authors： Abramovitch R,Neeman M,Reich R,Stein I,Keshet E,Abraham J,Solomon A,Marikovsky M

更新日期：1998-04-03 00:00:00

abstract：:To investigate interactions between the polychlorinated biphenyl-binding protein uteroglobin and phospholipids, we used a liposome-pelletting assay. PCB-BP/uteroglobin bound to liposomes made from negatively charged phospholipids (PtdSer and PtdIns) in the presence of 5 mM calcium. No binding to liposomes made from ph...

journal_title：FEBS letters

authors： Nord M,Gustafsson JA,Lund J

更新日期：1995-11-06 00:00:00

abstract：:Two RNA silencing-related phenomena, quelling and meiotic silencing by unpaired DNA (MSUD) have been identified in the fungus Neurospora crassa. Similar to the case with the siRNA and miRNA pathways in Drosophila, different sets of protein components including RNA-dependent RNA polymerase, argonaute and dicer, are use...

journal_title：FEBS letters

authors： Nakayashiki H

更新日期：2005-10-31 00:00:00

abstract：:Combining mutation and chemical modification, we have introduced Asp, Gln, Cys, S-carboxymethylcysteine (Cax) and S-carbamoylmethylcysteine (Cam) into the positions of Glu190 and Glu201 of the beta subunit of F1-ATPase from the thermophilic Bacillus PS3. The steady-state ATPase activities of alpha 3 beta 3 gamma compl...

journal_title：FEBS letters

authors： Amano T,Tozawa K,Yoshida M,Murakami H

更新日期：1994-07-04 00:00:00

abstract：:Disulfide bond formation is a critical step in the folding of many secretory proteins. In bacteria, disulfide bonds are introduced by the periplasmic dithiol oxidase DsbA, which transfers its catalytic disulfide bond to folding polypeptides. Reduced DsbA is reoxidized by ubiquinone Q8, catalyzed by inner membrane quin...

journal_title：FEBS letters

authors： Malojcić G,Owen RL,Grimshaw JP,Glockshuber R

更新日期：2008-10-15 00:00:00

abstract：:Cystatins A and C were both shown to inhibit cathepsin B by a two-step mechanism, involving an initial weak interaction followed by a conformational change. Disruption of the major salt bridge anchoring the occluding loop of cathepsin B to the main body of the enzyme by mutation of His110 to Ala converted the binding ...

journal_title：FEBS letters

authors： Pavlova A,Krupa JC,Mort JS,Abrahamson M,Björk I

更新日期：2000-12-29 00:00:00

abstract：:Crystals of methylamine-treated alpha 2-macroglobulin (alpha 2M-MA), alpha 2-macroglobulin in complex with two molecules of trypsin, alpha 2M-T2, one molecule of plasmin, alpha 2M-PL, and one molecule of plasmin followed by methylamine-treatment, alpha 2M-PL(MA), have reproducibly been obtained using ammonium sulfate ...

journal_title：FEBS letters

authors： Andersen GR,Jacobsen L,Thirup S,Nyborg J,Sottrup-Jensen L

更新日期：1991-11-04 00:00:00

abstract：:Mycobacterium tuberculosis contains multiple versions of the accA and accD genes that encode the alpha- and beta-subunits of at least three distinct multi-functional acyl-CoA carboxylase complexes. Because of its proposed involvement in pathogenic M. tuberculosis survival, the high-resolution crystal structure of the ...

journal_title：FEBS letters

authors： Holton SJ,King-Scott S,Nasser Eddine A,Kaufmann SH,Wilmanns M

更新日期：2006-12-22 00:00:00

abstract：:The nuclear gene OXA1 was first isolated in Saccharomyces cerevisiae and found to be required at a post-translational step in cytochrome c oxidase biogenesis, probably at the level of assembly. Mutations in OXA1 lead to a complete respiratory deficiency. The protein Oxa1p is conserved through evolution and a human hom...

journal_title：FEBS letters

authors： Altamura N,Capitanio N,Bonnefoy N,Papa S,Dujardin G

更新日期：1996-03-11 00:00:00

abstract：:Vpu is an 81 amino acid protein encoded by HIV-1. Its role is to amplify viral release by two mechanisms: (i) docking to CD4 with the consequence of targeting CD4 for ubiquitine-mediated degradation, and (ii) formation of ion channels to enhance particle release. The intensive research on its in vivo function, combine...

journal_title：FEBS letters

authors： Fischer WB

更新日期：2003-09-18 00:00:00

abstract：:The interconversions of the inositol-containing lipids (PI, PI-P and PI-P2) and their products (DG, inositol phosphates and PA) in human and rabbit neutrophils stimulated with fMetLeuPhe and PMA have been examined. PMA causes only the phosphorylation of PI to PI-P whereas fMetLeuPhe causes phosphorylation of both PI a...

journal_title：FEBS letters

authors： Cockcroft S,Barrowman MM,Gomperts BD

更新日期：1985-02-25 00:00:00

abstract：:One key area of protein kinase research is the identification of cognate substrates. The search for substrates is hampered by problems in unambiguously assigning substrates to a particular kinase in vitro and in vivo. One solution to this impasse is to engineer the kinase of interest to accept an ATP analogue which is...

journal_title：FEBS letters

authors： Hindley AD,Park S,Wang L,Shah K,Wang Y,Hu X,Shokat KM,Kolch W,Sedivy JM,Yeung KC

更新日期：2004-01-02 00:00:00

abstract：:Gangliosides and neutral glycolipids of muscles from normal and dystrophic chickens were studied. Total glycolipid content of the degenerating muscles was higher than the normal muscles. In addition, the myopathic muscles contained a ganglioside which was absent in the unaffected muscles from normal and dystrophic chi...

journal_title：FEBS letters

authors： Kundu SK,Misra LK,Luthra MG

更新日期：1982-12-27 00:00:00