Abstract:
:Thrombin has been shown to inhibit skeletal muscle differentiation. However, the mechanisms by which thrombin represses myogenesis remain unknown. Since the thrombin receptor couples to G(i), G(q/11) and G(12), we examined which subunits of heterotrimeric guanine nucleotide-binding regulatory proteins (Galpha(i), Galpha(q/11), Galpha(12) or Gbetagamma) participate in the thrombin-induced inhibition of C2C12 myoblast differentiation. Galpha(i2) and Galpha(11) had no inhibitory effect on the myogenic differentiation. Galpha(12) prevented only myoblast fusion, whereas Gbetagamma inhibited both the induction of skeletal muscle-specific markers and the myotube formation. In addition, the thrombin-induced reduction of creatine kinase activity was blocked by the C-terminal peptide of beta-adrenergic receptor kinase, which is known to sequester free Gbetagamma. These results suggest that the thrombin-induced inhibition of muscle differentiation is mainly mediated by Gbetagamma.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Nagao M,Kaziro Y,Itoh Hdoi
10.1016/s0014-5793(00)01458-7keywords:
subject
Has Abstractpub_date
2000-04-28 00:00:00pages
297-301issue
2-3eissn
0014-5793issn
1873-3468pii
S0014-5793(00)01458-7journal_volume
472pub_type
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