Abstract:
:The A-rich bulge in paired region P5a of the Tetrahymena intron is a structural feature that is conserved in the sub-group Ib self-splicing introns. We have constructed a series of substitution and deletion mutations in this region of the intron. Kinetic analysis has shown that some of the mutants have a reduced maximal extent of splicing, while others have a reduced Vmax. These mutations could be reactivated to a great extent by spermidine and high Mg2+ concentrations. These data are consistent with the hypothesis that the A-rich bulge of P5a has a role in stabilizing the higher-level structure of the ribozyme.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Pace U,Szostak JWdoi
10.1016/0014-5793(91)80230-zsubject
Has Abstractpub_date
1991-03-11 00:00:00pages
171-4issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(91)80230-Zjournal_volume
280pub_type
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