Abstract:
:Extraction and identification of the non-covalently bound heme groups from crude membrane preparations of photoheterotrophically grown Synechocystis sp. PCC 6803 by reversed phase high performance liquid chromatography and optical spectrophotometry led to the detection of heme O in addition to hemes B and A which latter was to be expected from the known presence of aa3-type cytochrome oxidase in cyanobacteria. In fully aerated cells (245 microM dissolved O2 in the medium) besides heme B only heme A was found while in low-oxygen cells (< 10 microM dissolved O2) heme O was present at a concentration even higher than that of heme A. Given the possible role of heme O as a biosynthetic intermediate between heme B and heme A, together with generally much higher Km values of 5-50 microM O2 for oxygenase as compared to Km values of 40-70 nM O2 for typical cytochrome-c oxidase, our findings may permit the conclusion that the conversion of heme O to heme A is an obligately oxygen-requiring process catalyzed by some oxygenase directly introducing oxygen from O2 into the 8-methyl group of heme O. At the same time thus the occurrence of heme O (cytochrome o) in cyanobacteria does of course not imply the existence of an 'alternative oxidase' since according to the well-known 'promiscuity of heme groups' both hemes O and A are likely to combine with one and the same apoprotein.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Peschek GA,Wastyn M,Fromwald S,Mayer Bdoi
10.1016/0014-5793(95)00821-psubject
Has Abstractpub_date
1995-09-04 00:00:00pages
89-93issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(95)00821-Pjournal_volume
371pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::We investigated 38 cases of B-cell chronic lymphocytic leukemia (B-CLL) for the presence of non-productive rearrangements in the S(mu) regions and defined for the first time the molecular nature of these rearrangements. Southern blot analysis revealed S(mu) region rearrangements in 13 cases (34%) and polymerase chain ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02672-8
更新日期:2002-05-08 00:00:00
abstract::Human monocyte-macrophages were incubated for 24 h in Ham's F-10 medium with human low-density lipoprotein (LDL) in the presence or absence of beta-carotene, canthaxanthin or zeaxanthin, at final concentrations of 2.5, 12.5 and 25 mg/l. LDL oxidation, measured by agarose gel electrophoresis, the thiobarbituric acid as...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01488-3
更新日期:1997-01-20 00:00:00
abstract::In hepatocytes from control rats, the ureogenic action of epinephrine is mainly mediated through alpha 1-adrenoceptors and the effect is independent of the presence of extracellular calcium. In hepatocytes from adrenalectomized rats, both alpha 1- and beta-adrenoceptors are involved in the action of epinephrine. Furth...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80118-0
更新日期:1984-01-30 00:00:00
abstract::A selective PKC inhibitor, UCN-01, was shown to exhibit anti-tumor activity in vitro and in vivo. We investigated UCN-01 with respect to isozyme-specific PKC inhibition using purified recombinant or rabbit brain PKC isozymes, cPKC alpha, beta and gamma, nPKC delta, epsilon and eta, and a PKC zeta. Of the PKC isozymes ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00042-8
更新日期:1995-02-13 00:00:00
abstract::It is known that narcotic antagonists interact with many cholinergic pathways but less in known about specific mechanisms. Using neonatal rat myoballs to study single channel behaviour of the acetylcholinegated nicotinic receptor, it was found that micromolar concentrations of naltrexone had no effect on channel condu...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)81145-5
更新日期:1985-03-11 00:00:00
abstract::LncRNAs have a critical role in the regulation of cellular processes such as cancer progression and metastasis. In the present study, we confirmed that TUG1 was overexpressed in bladder cancer tissues and established cell lines. Knockdown of TUG1 inhibited bladder cancer cell metastasis both in vitro and in vivo. Furt...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.08.020
更新日期:2015-10-07 00:00:00
abstract::The Ca2+ receptor mechanism of the parathyroid cell was studied using La3+ as a probe. La3+ was found to bind to the cell surface without further penetration. Measurements of 45Ca fluxes and the cytoplasmic Ca2+ concentration (Ca2+i) revealed a stimulatory component in the action of La3+ on Ca2+ permeability resulting...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80880-8
更新日期:1986-09-01 00:00:00
abstract::Cytochrome c oxidase is a membrane-bound redox-driven proton pump that harbors two proton-transfer pathways, D and K, which are used at different stages of the reaction cycle. Here, we address the question if a D pathway with a modified energy landscape for proton transfer could take over the role of the K pathway whe...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13679
更新日期:2020-03-01 00:00:00
abstract::The known functions of type II thioesterases (TEIIs) in type I polyketide synthases (PKSs) include selecting of starter acyl units, removal of aberrant extender acyl units, releasing of final products, and dehydration of polyketide intermediates. In this study, we characterized two TEIIs (ScnI and PKSIaTEII) from Stre...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.07.010
更新日期:2014-08-25 00:00:00
abstract::Following the biophysical analysis of plant K+ channels in their natural environment, three members from the green branch of the evolutionary tree of life KAT1, AKT1, and KST1 have recently been identified on the molecular level. Among them, we focussed on the expression and characterization of the Arabidopsis thalian...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00042-7
更新日期:1996-02-19 00:00:00
abstract::Benzimidazoles are widely used as anthelmintic agents and systemic fungicides. In susceptible organisms, benzimidazoles bind to beta-tubulin and block microtubule polymerization. To further characterize this interaction, site-directed mutagenesis followed by gene replacement was used to change Saccharomyces cerevisiae...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00334-1
更新日期:1996-04-29 00:00:00
abstract::A full-length cDNA coding for troponin-C isolated from an adult rabbit fast skeletal muscle library has been sequenced and expressed in an E. coli host. The amino acid sequence derived from the coding region agrees with the published protein sequence except that the first two residues are reversed. The expressed prote...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80576-3
更新日期:1988-02-08 00:00:00
abstract::The interactions of DNA duplexes with 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1), a potent mutacarcinogen isolated from tryptophan pyrolysate, have been studied using CD spectroscopy. The results are that (a) the spectral change of B-form DNA caused by the interaction with Trp-P-1 is biphasic, i.e. the enla...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80139-l
更新日期:1993-06-21 00:00:00
abstract::Turnip yellow mosaic virus (TYMV) is a positive-strand RNA virus able to infect Arabidopsis thaliana. To establish a TYMV infection system in Arabidopsis cell culture, TYMV replicons with the capsid protein gene replaced by a reporter gene expressing the Sh ble protein conferring zeocin resistance were used to transfe...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.12.045
更新日期:2007-01-23 00:00:00
abstract::The aim of this study was to evaluate, using a rat model of balloon angioplasty, whether prostaglandin (PG) J(2) and 2-cyclopenten-1-one are able to reduce restenosis. We found that both PGJ(2) and 2-cyclopenten-1-one, administered by local application on carotid arteries, caused a dose-dependent inhibition of neointi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00873-1
更新日期:2003-10-09 00:00:00
abstract::Oncogenic mutations disrupt the regulatory circuits that govern cell function, enabling tumor cells to undergo de-regulated mitogenesis, to resist to pro-apoptotic insults, and to invade through tissue boundaries. Cancer cell biology has played a crucial role in elucidating the signaling mechanisms by which oncogenic ...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2014.02.005
更新日期:2014-08-19 00:00:00
abstract::In order to identify physiological activators of proteinase-activated receptor-2 (PAR-2), a peptide chloromethane inhibitor (biotinyl-Ser-Lys-Gly-Arg-CH2Cl) based on the cleavage site for activation of PAR-2 was synthesised and tested with 12 trypsin-like serine proteinases. The second-order rate constant (ki/Ki) for ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01298-2
更新日期:1997-11-17 00:00:00
abstract::Members of the heat shock protein 70 (Hsp70) family are found in most of the compartments of eukaryotic cells where they play essential roles in protein metabolism. In yeast mitochondria, two Hsp70 proteins are known: Ssc1 and Ssq1. We identified Ecm10 as a third Hsp70 protein in the mitochondrial matrix. Ecm10 shares...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02364-4
更新日期:2000-12-29 00:00:00
abstract::Isolated rat islets of Langerhans permeabilised by high-voltage discharge secreted insulin in response to elevations in Ca2+ over the range 100 nM to 10 microM Ca2+. The phorbol ester, 12-O-tetradecanoylphorbol 13-acetate (TPA), had no effects on insulin secretion in the absence of Ca2+. In the presence of Ca2+ concen...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)81002-4
更新日期:1985-10-21 00:00:00
abstract::Platinum electrodes can be coated with cytochrome b5-phospholipid monolayers by the Langmuir-Blodgett technique. Cyclic voltammetry of a series of dyes shows that the coated electrodes become selective for certain electroactive species. The electron transfer reactions of negatively charged species are inhibited at the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81463-1
更新日期:1987-03-09 00:00:00
abstract::With the ultimate aim of developing an effective antiviral strategy against HIV-1, a mono-DNA enzyme possessing the 10-23 catalytic motif [Santoro and Joyce (1997) Proc. Natl. Acad. Sci. USA 94, 4264-4266] was synthesized against the HIV-1 envelope gene. We tested the in vitro cleavage efficiency of the 178 bp long tr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00799-6
更新日期:1998-07-24 00:00:00
abstract::RKIP was first identified as an inhibitor of the Raf-MEK-ERK signaling pathway. RKIP was also found to play an important role in the NF-kappaB pathway. Genetic and biochemical studies demonstrated that RKIP functioned as a scaffold protein facilitating the phosphorylation of IkappaB by upstream kinases. However, contr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.12.051
更新日期:2010-02-19 00:00:00
abstract::To identify the linear epitope for Fc-binding on the bovine IgG2 Fc receptor (boFcgamma2R), peptides derived from the membrane-distal extracellular domain (EC1) of boFcgamma2R corresponding to the homologous region of human FcalphaRI were synthesized. Binding of bovine IgG2 to the different peptides was tested by Dot-...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.01.060
更新日期:2006-02-20 00:00:00
abstract::A new 175-kDa membrane protein was isolated from chicken gizzard smooth muscle. Antibodies to 175-kDa protein were used for localization of this protein in smooth and cardiac muscles. In both types of muscle 175-kDa protein was localized near plasma membrane. 175-kDa protein was able to interact specifically with vinc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)81155-8
更新日期:1985-03-11 00:00:00
abstract::The transcriptional start site of the human cholecystokinin (CCK)-A receptor gene was determined by the Capsite Hunting method. Two sequence changes were detected, a G to T change in nucleotide -128, and an A to G change in nucleotide -81. The homozygote (T/T, G/G) was detected in 25 of 1296 individuals (1.9%) in the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01080-2
更新日期:2000-01-28 00:00:00
abstract::The gene for the extracellular ribonuclease of B. pumilus KMM62 (RNase Bp) has been cloned and sequenced. The structural gene for this enzyme is similar to those of the extracellular ribonucleases of B. intermedius 7P (binase) and B. amyloliquefaciens H2 (barnase), as are the regulatory regions of binase and RNase Bp....
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01316-s
更新日期:1995-01-02 00:00:00
abstract::A bovine anterior pituitary polypeptide that stimulates the replication of rat and human adipocyte precursors has been purified. Its Mr is 44 000-53 000 and its isoelectric point is 9.8-10.3. While pituitary basic fibroblast growth factor is equally mitogenic on adipocyte precursors and skin fibroblasts, the polypepti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80651-6
更新日期:1983-03-21 00:00:00
abstract::The structure of the Fab fragment of the monoclonal antibody MAK 33 (kappa/IgG1) at pH 2 was characterized. Spectroscopic and kinetic analysis revealed a molten globule-like state, characterized by elements of secondary structure but less defined tertiary contacts than in the native state. However, some aromatic side ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00203-l
更新日期:1995-03-27 00:00:00
abstract::Using the patch-clamp technique, we demonstrate here the opening of K+ channels evoked by the actively transported amino acid L-alanine in isolated Necturus enterocytes. These channels had a conductance of about 30 pS and their activation was dependent on transmembrane electrical potential and cytosolic Ca2+. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80134-0
更新日期:1988-07-18 00:00:00
abstract::Syntenin has crucial roles in cell adhesion, cell migration and synaptic transmission. Its closely linked postsynaptic density-95, discs large 1, zonula occludens-1 (PDZ) domains typically interact with C-terminal ligands. We profile syntenin PDZ1-2 through proteomic peptide phage display (ProP-PD) using a library tha...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.12037
更新日期:2016-01-01 00:00:00