Abstract:
:Members of the heat shock protein 70 (Hsp70) family are found in most of the compartments of eukaryotic cells where they play essential roles in protein metabolism. In yeast mitochondria, two Hsp70 proteins are known: Ssc1 and Ssq1. We identified Ecm10 as a third Hsp70 protein in the mitochondrial matrix. Ecm10 shares 82% amino acid identity with Ssc1 and 54% with Ssq1. Overexpression of Ecm10 mitigates protein import defects in ssc1 mutants suggesting that Ecm10 can play a role in protein translocation. Like Ssc1, Ecm10 interacts with the nucleotide exchange factor Mge1 in an ATP-dependent manner. Deletion of ecm10 leads to synthetic growth defects with ssc1 mutations at low temperature. Our data suggest an overlapping function of Ecm10 and Ssc1.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Baumann F,Milisav I,Neupert W,Herrmann JMdoi
10.1016/s0014-5793(00)02364-4keywords:
subject
Has Abstractpub_date
2000-12-29 00:00:00pages
307-12issue
2eissn
0014-5793issn
1873-3468pii
S0014-5793(00)02364-4journal_volume
487pub_type
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