Abstract:
:Phosphatidylinositol 3 kinase (PI-3 kinase) activity has been linked to cell proliferation and growth regulation. Therefore, we studied changes in phosphoinositide metabolism during the cell cycle of HT-29 cells, a colonic epithelial cell line. HT29 cells were treated with the microtubule disrupter, nocodozole, separated into mitotic and quiescent populations and their phospholipid composition was analyzed. Radiolabelled phospholipids from cells labelled with 32PO4 or [3H]myoinositol were analyzed by TLC and/or deacylated and analyzed by HPLC. In all cases, levels of phosphatidylinositol 3 phosphate from mitotic phase cells were double that in resting populations. Therefore, levels of a product of PI-3 kinase are elevated and may play a role in cell division.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Dobos GJ,Wu XR,Traynor-Kaplan Adoi
10.1016/0014-5793(93)81381-9subject
Has Abstractpub_date
1993-06-14 00:00:00pages
143-6issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(93)81381-9journal_volume
324pub_type
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