Identification of casein kinase Ialpha interacting protein partners.

abstract：:Polymerising proteins of the actin family are nearly ubiquitous. Crenactins, restricted to Crenarchaea, are more closely related to actin than bacterial MreB. Crenactins occur in gene clusters hinting at an unknown, but conserved function. We solved the crystal structure of crenactin at 3.2 Å resolution. The protein c...

journal_title：FEBS letters

authors： Izoré T,Duman R,Kureisaite-Ciziene D,Löwe J

更新日期：2014-03-03 00:00:00

abstract：:Gene conservation, duplication and constitutive expression are intricately linked and strong predictors of essentiality. Here, we introduce metrics based on diversity indices to measure gene conservation, duplication and constitutive expression and validate them by measuring their performance in prediction of essentia...

journal_title：FEBS letters

authors： Arun PV,Miryala SK,Chattopadhyay S,Thiyyagura K,Bawa P,Bhattacharjee M,Yellaboina S

更新日期：2016-05-01 00:00:00

abstract：:The specific binding of [3H]retinoids to cellular retinoid-binding proteins was measured directly by the cold acetone filtration method. After incubation of purified cellular retinoid-binding proteins with [3H]retinoids with or without competitors for 2-4 h, bound ligands were separated from free by filtration using c...

journal_title：FEBS letters

authors： Ninomiya M,Suganuma M,Paik NS,Muto Y,Fujiki H

更新日期：1988-06-20 00:00:00

abstract：:The sequence of the 18 S rRNA of Tenebrio molitor is reported. A detailed secondary structure model for eukaryotic small subunit rRNAs is proposed. The model comprises 48 universal helices that eukaryotic and prokaryotic small subunit rRNAs have in common, plus a number of helices in areas of variable secondary struct...

journal_title：FEBS letters

authors： Hendriks L,De Baere R,Van Broeckhoven C,De Wachter R

更新日期：1988-05-09 00:00:00

abstract：:The post-translational modification of histone proteins plays an important role in controlling cell fate by directing essentially all DNA-associated nuclear processes. Misregulation and mutation of histone modifying enzymes is a hallmark of tumorigenesis. However, how these different epigenetic modifications lead to t...

journal_title：FEBS letters

authors： Johnsen SA

更新日期：2012-06-04 00:00:00

abstract：:Neopterin is synthesized by human monocyte-derived macrophages primarily upon stimulation with the cytokine interferon-gamma. We studied the influence of neopterin on the generation of reactive oxygen species (ROS) in human peripheral blood neutrophils. Radical formation was measured using a biochemiluminometer. Neutr...

journal_title：FEBS letters

authors： Razumovitch JA,Semenkova GN,Fuchs D,Cherenkevich SN

更新日期：2003-08-14 00:00:00

abstract：:Terminal oligopyrimidine (TOP) mRNAs are a group of messengers translationally regulated according to the growth status of the cell. Two hypotheses have been proposed for the mechanism of the regulation: (i) there is a specific translational regulator which can reversibly alter TOP-mRNA structure, (ii) a component of ...

journal_title：FEBS letters

authors： Loreni F,Amaldi F

更新日期：1997-10-27 00:00:00

abstract：:A bark lectin, RBL, from Robinia pseudoacacia (black locust), binds galactose-related sugars specifically. Recombinant RBL (rRBL) with a histidine tag was expressed in Escherichia coli, purified and characterized. rRBL agglutinated rabbit erythrocytes and the hemagglutination was inhibited by galactose and related sug...

journal_title：FEBS letters

authors： Nishiguchi M,Yoshida K,Sumizono T,Tazaki K

更新日期：1997-02-24 00:00:00

abstract：:Thymidine kinase in chick embryo retina reaches its highest values on the 8-10th day of development, then declines reaching the lowest value at hatching. The rate of DNA synthesis essentially follows this activity while, in contrast, nucleoside phosphotransferase increases progressively during development. Glucocortic...

journal_title：FEBS letters

authors： Tesoriere G,Tesoriere L,Giuliano M,Vento R

更新日期：1983-11-28 00:00:00

abstract：:Two cDNA clones encoding 14-3-3 homologous proteins were isolated from Vicia faba. Deduced amino acid sequences share different degrees of homology with other plant 14-3-3 proteins. Both clones, under the control of the CaMV 35S promoter, were transformed into tobacco plants. Immunoblotting showed three different form...

journal_title：FEBS letters

authors： Saalbach G,Schwerdel M,Natura G,Buschmann P,Christov V,Dahse I

更新日期：1997-08-18 00:00:00

abstract：:The effect of lysophosphatidic acid (LPA) on the preimplantation development of mouse embryos was examined. The blastocyst rates from 2-cell and 4-cell stage embryos were significantly higher in the presence of LPA at the pronuclear stage. However, the blastocyst rate from 4-cell stage embryos was significantly lower ...

journal_title：FEBS letters

authors： Kobayashi T,Yamano S,Murayama S,Ishikawa H,Tokumura A,Aono T

更新日期：1994-08-29 00:00:00

abstract：:A subcellular fraction, highly enriched in uncoated vesicles (UCV) with high H+-ATPase (EC 3.6.1.34) activity, was isolated from the crude microsomal fraction of rat liver homogenates by discontinuous sucrose gradient centrifugation. The UCV fraction, recovered at the interface of sucrose density 1.08 and 1.10 g/ml, w...

journal_title：FEBS letters

authors： Flatmark T,Haavik J,Grønberg M,Jorunn Kleiveland L,Wahlstrøm Jacobsen S,Berge SV

更新日期：1985-09-02 00:00:00

abstract：:The number of muscarinic acetylcholine receptors (MAChRs), as detected by binding of [3H]quinuclidinyl benzilate (QNB), was investigated under conditions which promote protein phosphorylation. Incubation of a crude heart membrane preparation in the presence of ATP/Mg2+ reduced MAChR number by 50%. Incubation with poly...

journal_title：FEBS letters

authors： Creazzo TL,Wrenn RW

更新日期：1988-12-19 00:00:00

abstract：:There is strong evidence of a key role for mitochondrial dysfunction in both sporadic and all forms of familial Parkinson's disease (PD). However, none of the clinical trials carried out with putative mitochondrial rescue agents have been successful. Firm establishment of a wet biomarker or a reliable readout from ima...

journal_title：FEBS letters

authors： Mortiboys H,Macdonald R,Payne T,Sassani M,Jenkins T,Bandmann O

更新日期：2018-03-01 00:00:00

abstract：:Using a variety of peptide analogues of oxytocin (OT) and Arg8-vasopressin (AVP), OT-mediated induction of urokinase-type plasminogen activator (uPA) was examined in LLC-PK1 renal epithelial cells, which possess distinct high-affinity receptors of both the OT- and vasopressin renal (V2-) types. OT or OT-receptor speci...

journal_title：FEBS letters

authors： Jans DA,Pavo I,Fahrenholz F

更新日期：1993-01-04 00:00:00

abstract：:Mutual arrangement of histone H1 molecules in chromatin extended in low salt-EDTA buffer and additionally in the presence of urea was studied by means of reversible cross-linking combined with chymotryptic digestion. In the chromatins tested, the chymotryptic halves of H1 were cross-linked in all possible combinations...

journal_title：FEBS letters

authors： Nikolaev LG,Glotov BO,Dashkevich VK,Barbashov SF,Severin ES

更新日期：1983-10-31 00:00:00

abstract：:The effect of oxLDL on CD36 expression has been assessed in preadipocytes induced to differentiate. Novel evidence is provided that oxLDL induce a peroxisome proliferator-activated receptor gamma-independent CD36 overexpression, by up-regulating nuclear factor erythroid 2 (NF-E2)-related factor 2 (Nrf2). The nuclear t...

journal_title：FEBS letters

authors： D'Archivio M,Scazzocchio B,Filesi C,Varì R,Maggiorella MT,Sernicola L,Santangelo C,Giovannini C,Masella R

更新日期：2008-06-25 00:00:00

abstract：:Rho GTPases participate in various important signaling pathways and have been implicated in myogenic differentiation. Here the first evidence is provided that in C2C12 myoblasts sphingosine 1-phosphate (SPP) rapidly and transiently induced membrane association of Rho A in a pertussis toxin-insensitive manner. The bioa...

journal_title：FEBS letters

authors： Meacci E,Donati C,Cencetti F,Romiti E,Bruni P

更新日期：2000-09-29 00:00:00

abstract：:The O2-dependent formation of side products during the oxidation of veratryl alcohol (VA) by lignin peroxidase has previously been proposed to start with the attack of H2O on the VA radical cation (VA*+). This initial reaction is unlikely since it would also lead to side product formation in the absence of O2, which i...

journal_title：FEBS letters

authors： ten Have R,Franssen MC

更新日期：2001-01-05 00:00:00

abstract：:Acrosin inhibitors of seminal vesicle origin, after binding to their acceptor molecules on the anterior part of ejaculated sperm, are thought to be important capacitation factors, protecting zona binding sites during sperm uterine passage, and then dissociating to allow sperm binding to the zona pellucida of the oocyt...

journal_title：FEBS letters

authors： Jonáková V,Calvete JJ,Mann K,Schäfer W,Schmid ER,Töpfer-Petersen E

更新日期：1992-02-03 00:00:00

abstract：:The red fluorescent protein, DsRed, recently cloned from coral Discosoma sp. has one of the longest fluorescence waves and one of the most complex absorbance spectra among the family of fluorescent proteins. In this work we found that with time DsRed fluorescence decreases under mildly acidic conditions (pH 4.0-4.8) i...

journal_title：FEBS letters

authors： Vrzheshch PV,Akovbian NA,Varfolomeyev SD,Verkhusha VV

更新日期：2000-12-29 00:00:00

abstract：:Messenger RNA from intact rat pancreatic islets, or from transformed hamster beta (HIT) cells, hybridized with the cDNA probe for type I (but not type II) phospholipase A2. The levels of phospholipase A2 mRNA increased in islets from fasted rats; they decreased in islets cultured in a high glucose concentration (contr...

journal_title：FEBS letters

authors： Metz S,Holmes D,Robertson RP,Leitner W,Draznin B

更新日期：1991-12-16 00:00:00

abstract：:Flash excitation of Tris-treated photosystem II (PSII) membranes generated a thermoluminescence band peaking at -20 degrees C, indicating that a positively charged oxidizing equivalent is stably accumulated on donor side of PSII even in the absence of the Mn-cluster. This oxidizing equivalent was sensitive to low conc...

journal_title：FEBS letters

authors： Ono T,Inoue Y

更新日期：1991-01-28 00:00:00

abstract：:A novel protein that binds specifically to the intracellular domain of the p55 tumor necrosis factor (TNF) receptor was cloned by two-hybrid screening of a HeLa cell cDNA library. Data bank searches revealed high sequence similarity of the protein (55.11) to yeast, nematode and plant proteins, whose functions are yet ...

journal_title：FEBS letters

authors： Boldin MP,Mett IL,Wallach D

更新日期：1995-06-19 00:00:00

abstract：:The Pb, Eu and Mg-induced cleavages in E. coli and lupine tRNAPhe have been characterized and compared with those found in yeast tRNAPhe. The pattern of lupine tRNAPhe hydrolysis closely resembles that of yeast tRNAPhe, while several major differences occur in the specificity and efficiency of the E. coli tRNAPhe hydr...

journal_title：FEBS letters

authors： Marciniec T,Ciesiołka J,Wrzesinski J,Krzyzosiak WJ

更新日期：1989-01-30 00:00:00

abstract：:NDRG1 is phosphorylated by SGK1 (but not PKB) in vivo at three residues each contained within three nonapeptide repeats. Here, we demonstrate that this nonapeptide, like the NDRG1 protein, is phosphorylated by SGK1, but not by PKBalpha or RSK1 in vitro. The inability of PKBalpha and RSK1 to phosphorylate the nonapepti...

journal_title：FEBS letters

authors： Murray JT,Cummings LA,Bloomberg GB,Cohen P

更新日期：2005-02-14 00:00:00

abstract：:Here we show that increased amount of secondary structure is acquired in the folded states of two structurally-different proteins (alpha-helical VlsE and alpha/beta flavodoxin) in the presence of macromolecular crowding agents. The structural content of flavodoxin and VlsE is enhanced by 33% and 70%, respectively, in ...

journal_title：FEBS letters

authors： Perham M,Stagg L,Wittung-Stafshede P

更新日期：2007-10-30 00:00:00

abstract：:Homocysteine thiolactone is a product of an error-editing reaction, catalyzed by Escherichia coli and Saccharomyces cerevisiae methionyl-tRNA synthetases, which prevents incorporation of homocysteine into tRNA and protein both in vitro and in vivo. Here, homocysteine thiolactone is also shown to be synthesized by cult...

journal_title：FEBS letters

authors： Jakubowski H,Goldman E

更新日期：1993-02-15 00:00:00

abstract：:Mediatophore is a protein purified from the nerve terminal membranes of Torpedo electric organ. It confers to artificial membranes a calcium-dependent mechanism that translocates acetylcholine. When similar reconstitution experiments are applied to rat brain synaptosomal membranes they reveal the presence of mediatoph...

journal_title：FEBS letters

authors： Israël M,Lesbats B,Morel N,Manaranche R,Le Gal la Salle G

更新日期：1988-06-20 00:00:00

abstract：:A wide variety of phospholipase A2 inhibitors blocks intra-Golgi protein transport reconstituted in a cell-free system. Phospholipase A2 activity detectable under the protein transport assay conditions is actually inhibited by the inhibitors. There is a good correlation between the inhibition of protein transport and ...

journal_title：FEBS letters

authors： Tagaya M,Henomatsu N,Yoshimori T,Yamamoto A,Tashiro Y,Fukui T

更新日期：1993-06-14 00:00:00