Abstract:
:Polymerising proteins of the actin family are nearly ubiquitous. Crenactins, restricted to Crenarchaea, are more closely related to actin than bacterial MreB. Crenactins occur in gene clusters hinting at an unknown, but conserved function. We solved the crystal structure of crenactin at 3.2 Å resolution. The protein crystallises as a continuous right-handed helix with 8 subunits per complete turn, spanning 419 Å. The structure of crenactin shows several loops that are longer than in actin, but overall, crenactin is closely related to eukaryotic actin, with an RMSD of 1.6 Å. Crenactin filaments imaged by electron microscopy showed polymers with very similar helical parameters.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Izoré T,Duman R,Kureisaite-Ciziene D,Löwe Jdoi
10.1016/j.febslet.2014.01.029subject
Has Abstractpub_date
2014-03-03 00:00:00pages
776-82issue
5eissn
0014-5793issn
1873-3468pii
S0014-5793(14)00058-1journal_volume
588pub_type
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