Identification and functional analysis of essential, conserved, housekeeping and duplicated genes.

abstract：:The redox potentials (E(composite function')) of P700 in intact and diethyl ether-treated thylakoid membranes as well as native photosystem (PS) I particles from spinach and Thermosynechococcus elongatus have been measured by a spectroelectrochemistry with an error range of +/-2-3 mV. Stepwise removal of antenna pigme...

journal_title：FEBS letters

authors： Zhang Y,Nakamura A,Kuroiwa Y,Kato Y,Watanabe T

更新日期：2008-04-02 00:00:00

abstract：:Lipopolysaccharide, the invariant structural component of Gram-negative bacteria, when present in minute amounts in the circulation in humans elicits 'endotoxic shock' syndrome, which is fatal in 60% of the cases. Polymyxin B (PMB), a cyclic cationic peptide, neutralizes the endotoxin, but also induces many harmful si...

journal_title：FEBS letters

authors： Thomas CJ,Surolia A

更新日期：1999-02-26 00:00:00

abstract：:Lipid metabolism is extensively studied in Saccharomyces cerevisiae. Here, we report that revertant of glycogen synthase kinase mutation-1 (Rog1p) possesses monoacylglycerol (MAG) lipase activity in S. cerevisiae. The lipase activity of Rog1p was confirmed in two ways: through analysis of a strain with a double deleti...

journal_title：FEBS letters

authors： Vishnu Varthini L,Selvaraju K,Srinivasan M,Nachiappan V

更新日期：2015-01-02 00:00:00

abstract：:The FMN-heme interdomain (intraprotein) electron transfer (IET) kinetics in full length and oxygenase/FMN (oxyFMN) construct of human iNOS were determined by laser flash photolysis over the temperature range from 283 to 304K. An appreciable increase in the rate constant value was observed with an increase in the tempe...

journal_title：FEBS letters

authors： Li W,Chen L,Fan W,Feng C

更新日期：2012-01-20 00:00:00

abstract：:A number of human diseases can be linked to aberrations in protein folding which cause an imbalance in protein homeostasis. Molecular chaperones, including heat shock proteins, act to assist protein folding, stability and activity in the cell. Attention has begun to focus on modulating the expression and/or activity o...

journal_title：FEBS letters

authors： Powers MV,Workman P

更新日期：2007-07-31 00:00:00

abstract：:A cDNA of a novel form of type V adenylyl cyclase has been cloned from rabbit myocardium using oligonucleotide probes derived from peptides that were produced by enzymatic cleavage of purified heart cyclase. A corresponding mRNA (6 kb) has been detected in rabbit myocardial tissue by Northern blot analysis. The cDNA e...

journal_title：FEBS letters

authors： Wallach J,Droste M,Kluxen FW,Pfeuffer T,Frank R

更新日期：1994-02-07 00:00:00

abstract：:Ischemic preconditioning (IPrec) improves post-ischemic dysfunctions of the myocardium along with activation of protein kinase C isozymes including PKCdelta. Moreover, expression of cardio-protective determinants can reduce ischemic damages. Because IPrec is limited in aged hearts, we assessed in an experimental model...

journal_title：FEBS letters

authors： Bartling B,Hilgefort C,Friedrich I,Silber RE,Simm A

更新日期：2003-12-18 00:00:00

abstract：:Compared with algal and cyanobacterial cytochrome c(6), cytochrome c(6A) from higher plants contains an additional loop of 12 amino acid residues. We have determined the first crystal structure of cytochrome c(6A) from Arabidopsis thaliana at 1.5 Angstrom resolution in order to help elucidate its function. The overall...

journal_title：FEBS letters

authors： Chida H,Yokoyama T,Kawai F,Nakazawa A,Akazaki H,Takayama Y,Hirano T,Suruga K,Satoh T,Yamada S,Kawachi R,Unzai S,Nishio T,Park SY,Oku T

更新日期：2006-06-26 00:00:00

abstract：:Phosphorylation of the basal site with glycogen synthase kinase-4 enhanced the rate of phosphorylation of the regulatory site by cyclic AMP-dependent protein kinase 1.7-fold. In contrast, the phosphorylation state of the regulatory site did not affect the rate of phosphorylation of the basal site with glycogen synthas...

journal_title：FEBS letters

authors： Olsson H,Belfrage P

更新日期：1988-05-09 00:00:00

abstract：:The phylogenetic distribution of divergently related protein families into the three domains of life (archaea, bacteria and eukaryotes) can signify the presence or absence of entire cellular processes in these domains and their ancestors. We can thus study the emergence of the major transitions during cellular evoluti...

journal_title：FEBS letters

authors： Ouzounis C,Kyrpides N

更新日期：1996-07-22 00:00:00

abstract：:The covalent conjugation of oligonucleotides to antibody Fab' fragments was optimized by using oligonucleotides modified with a hexaethylene linker arm bearing three amino groups. One oligonucleotide was coupled to antibody of one specificity and a complementary oligonucleotide to antibody of a second specificity. The...

journal_title：FEBS letters

authors： Chaudri ZN,Bartlet-Jones M,Panayotou G,Klonisch T,Roitt IM,Lund T,Delves PJ

更新日期：1999-04-30 00:00:00

abstract：:The 42 kDa precursor of surfactant protein B generates the 79 residue mature SP-B polypeptide, which belongs to the family of saposin-like proteins and has unique functional roles in pulmonary surfactant. From sequence comparisons it has been suggested that proSP-B, in addition to SP-B, contains two saposin-like domai...

journal_title：FEBS letters

authors： Zaltash S,Johansson J

更新日期：1998-02-13 00:00:00

abstract：:Resistance to antimalarial drugs has often threatened malaria elimination efforts and historically has led to the short-term resurgence of malaria incidences and deaths. With concentrated malaria eradication efforts currently underway, monitoring drug resistance in clinical settings complemented by in vitro drug susce...

journal_title：FEBS letters

authors： Petersen I,Eastman R,Lanzer M

更新日期：2011-06-06 00:00:00

abstract：:By analyzing human embryonic kidney 293 cell transfectants stably overexpressing various types of phospholipase A2 (PLA2), we have shown that polyunsaturated fatty acids (PUFAs) preferentially activate type IIA secretory PLA2 (sPLA2-IIA)-mediated arachidonic acid (AA) release from interleukin-1 (IL-1)-stimulated cells...

journal_title：FEBS letters

authors： Kambe T,Murakami M,Kudo I

更新日期：1999-06-18 00:00:00

abstract：:In this study, we investigated enzymatic deglycosylation of glucoamylase from Aspergillus awamori X 100/D27, a glycoprotein which has two N-linked and about forty short mannose-bearing O-linked sugars per molecule. O-Linked sugars were modified by treatment with alpha-mannosidase and N-linked sugars were removed using...

journal_title：FEBS letters

authors： Neustroev KN,Golubev AM,Firsov LM,Ibatullin FM,Protasevich II,Makarov AA

更新日期：1993-01-25 00:00:00

abstract：:Escherichia coli maltose-binding protein (MBP) is frequently used as an affinity tag to facilitate the purification of recombinant proteins. An important additional attribute of MBP is its remarkable ability to enhance the solubility of its fusion partners. MBPs are present in a wide variety of microorganisms includin...

journal_title：FEBS letters

authors： Fox JD,Routzahn KM,Bucher MH,Waugh DS

更新日期：2003-02-27 00:00:00

abstract：:Endothelial dysfunction plays a major role in the pathogenesis of atherosclerosis. Pro-inflammatory cytokines such as interleukin-1 beta and tumour necrosis factor alpha activate endothelial cells changing their resting phenotype to become pro-adhesive, pro-thrombotic and pro-atherogenic. Phage display in vivo biopann...

journal_title：FEBS letters

authors： Houston P,Goodman J,Lewis A,Campbell CJ,Braddock M

更新日期：2001-03-09 00:00:00

abstract：:It is well known that, in rabbits, the allotypes of the b locus carried by the light chains of the IgG molecules cross-react. Nothing similar has been described for the allotypes of the a locus carried by the heavy chains of the IgG molecules. This paper describes a cross-reaction between the rabbit allotypic markers ...

journal_title：FEBS letters

authors： Renneboog-Squilbin C

更新日期：1969-02-01 00:00:00

abstract：:The interferon inducible transmembrane (IFITM) proteins mediate several cellular processes such as homotypic cell adhesion functions of interferons (IFNs) and cellular anti-proliferative activities. We show that the BAF complex-mediated induction of IFITM3 is dependent on binding of the transcriptional enhancer factor...

journal_title：FEBS letters

authors： Cuddapah S,Cui K,Zhao K

更新日期：2008-01-23 00:00:00

abstract：:Limb girdle muscular dystrophy type 2A is linked to a skeletal muscle-specific calpain isoform known as p94. Isolation of the intact 94-kDa enzyme has been difficult to achieve due to its rapid autolysis, and uncertainty has arisen over its Ca2+-dependence for activity. We have expressed a C-terminally truncated form ...

journal_title：FEBS letters

authors： Rey MA,Davies PL

更新日期：2002-12-18 00:00:00

abstract：:DNA methylation has a profound impact on the regulation of gene expression in normal cell development, and aberrant methylation has been recognized as a key factor in the pathogenesis of human diseases such as cancer. The discovery of modified nucleobases arising from 5-methylcytosine (5mC) through consecutive oxidati...

journal_title：FEBS letters

authors： Dietzsch J,Feineis D,Höbartner C

更新日期：2018-06-01 00:00:00

abstract：:Duchenne muscular dystrophy (DMD) is a fatal disorder caused by absence of functional dystrophin protein. Compensation in dystrophin-deficient (mdx) mice may be achieved by overexpression of its fetal paralogue, utrophin. Strategies to increase utrophin levels by stimulating promoter activity using small compounds are...

journal_title：FEBS letters

authors： Perkins KJ,Davies KE

更新日期：2018-06-01 00:00:00

abstract：:The TSH-induced cyclic AMP response was studied using a 3-year-old ovine thyroid cell line TSH-independent for growth: OVNIS 5H. The kinetics of cyclic AMP production was followed both in cell layers and in cell culture media, with or without phosphodiesterase inhibitor. It is noteworthy that following the first wave ...

journal_title：FEBS letters

authors： Fayet G,Aouani A,Hovsépian S

更新日期：1986-01-06 00:00:00

abstract：:In Graves' disease (GD) the presence of antibodies to the thyroid stimulating hormone (TSH) receptor leads to stimulation of the thyroid gland. The thyroid stimulating activity of Graves' IgG is normally ascertained by bioassays measuring cAMP production. We have investigated the effect of Graves' IgG on the quantitat...

journal_title：FEBS letters

authors： Kung AW,Collison K,Banga JP,McGregor AM

更新日期：1988-05-09 00:00:00

abstract：:The kinetics of the tRNA cycle is in itself capable of keeping the translational error level almost unaffected by amino acid starvation. There is no need to assume any yet unknown mechanism or property. Kinetic analysis shows that the concentration of aminoacyl-tRNA can stay high even for large reductions in aminoacyl...

journal_title：FEBS letters

authors： Liljenström H

更新日期：1987-10-19 00:00:00

abstract：:Syndecans are transmembrane heparan sulfate proteoglycans, with roles in development, tumorigenesis and inflammation, and growing evidence for involvement in tissue regeneration. This is a fast developing field with the prospect of utilizing tissue engineering and biomaterials in novel therapies. Syndecan receptors ar...

journal_title：FEBS letters

authors： Chung H,Multhaupt HA,Oh ES,Couchman JR

更新日期：2016-08-01 00:00:00

abstract：:The complete primary structure (967 amino acids) of an intestinal human aminopeptidase N (EC 3.4.11.2) was deduced from the sequence of a cDNA clone. Aminopeptidase N is anchored to the microvillar membrane via an uncleaved signal for membrane insertion. A domain constituting amino acid 250-555 positioned within the c...

journal_title：FEBS letters

authors： Olsen J,Cowell GM,Kønigshøfer E,Danielsen EM,Møller J,Laustsen L,Hansen OC,Welinder KG,Engberg J,Hunziker W

更新日期：1988-10-10 00:00:00

abstract：:Cellulysin, a crude cellulase from the plant parasitic fungus Trichoderma viride, induces the biosynthesis of volatiles in higher plants (Nicotiana plumbaginifolia, Phaseolus lunatus, and Zea mays) when applied to cut petioles by the transpiration stream. The pattern of the emitted volatiles largely resembles that fro...

journal_title：FEBS letters

authors： Piel J,Atzorn R,Gäbler R,Kühnemann F,Boland W

更新日期：1997-10-20 00:00:00

abstract：:Intracellular accumulation of anthracycline derivatives was measured in a human embryonic kidney cell line (HEK) and a resistant subline (HEK/multidrug resistance protein (MRP1)) overexpressing MRP1 at the plasma membrane surface. Two compounds (daunorubicin and doxorubicin) were rejected outside the multidrug-resista...

journal_title：FEBS letters

authors： Manciu L,Chang X,Riordan JR,Buyse F,Ruysschaert JM

更新日期：2001-03-23 00:00:00

abstract：:In plants exposed to high irradiances of visible light, the D1 protein in the reaction center of photosystem II is oxidatively damaged and rapidly degraded. Earlier work in our laboratory showed that the serine protease Deg2 performs the primary cleavage of photodamaged D1 protein in vitro. Here, we demonstrate that t...

journal_title：FEBS letters

authors： Huesgen PF,Schuhmann H,Adamska I

更新日期：2006-12-22 00:00:00