Dual specificity antibodies using a double-stranded oligonucleotide bridge.

abstract：:2-( Dimethylaminostyryl )-l-methyl pyridinium, a fluorescent indicator of mitochondrial membrane potential, is accumulated up to 400-fold by intact, isolated brown adipocytes from cold-adapted guinea pigs. The indicator can be optically localized within the in situ mitochondria. Ionophores which would be predicted to ...

journal_title：FEBS letters

authors： Rafael J,Nicholls DG

更新日期：1984-05-07 00:00:00

abstract：:The alpha-subunit of the TRAP complex (TRAP alpha) is a single-spanning membrane protein of the endoplasmic reticulum (ER) which is found in proximity of nascent polypeptide chains translocating across the membrane. Here, we demonstrate the widespread occurrence of TRAP alpha in eukaryotes as indicated by its existenc...

journal_title：FEBS letters

authors： Hartmann E,Prehn S

更新日期：1994-08-08 00:00:00

abstract：:A rat urokinase receptor (uPAR) cDNA fragment was amplified by RT-PCR from RNA of the rat osteoblastic cell line CFK-1. Using this DNA species as a hybridization probe two rat uPAR cDNAs were isolated from a CFK-1 cDNA library. These two cDNAs encode an identical uPAR protein except for a single base mutation which re...

journal_title：FEBS letters

authors： Rabbani SA,Rajwans N,Achbarou A,Murthy KK,Goltzman D

更新日期：1994-01-24 00:00:00

abstract：:Cytoplasmic colicin A has the ability to bind to membranes and to form stable dimers. This form remains stable even in the presence of 1% SDS at 25 degrees C. Both of these properties were not observed for extracellular colicin A suggesting a possible difference in the conformation between cytoplasmic and extracellula...

journal_title：FEBS letters

authors： Knibiehler M,Lazdunski C

更新日期：1987-06-01 00:00:00

abstract：:The human H19 gene is a paternally imprinted oncofetal gene, highly expressed in several fetal tissues, down-regulated in nearly all adult tissues but re-expressed in carcinomas of tissues which express the gene in fetal life. It has no known protein product and till today, no function could be designated to H19 RNA. ...

journal_title：FEBS letters

authors： Ohana P,Kopf E,Bibi O,Ayesh S,Schneider T,Laster M,Tykocinski M,de Groot N,Hochberg A

更新日期：1999-07-02 00:00:00

abstract：:The beta-adrenergic receptor kinase mediates agonist-dependent phosphorylation of beta-adrenergic receptors, which is thought to represent the first step of homologous desensitization. We have expressed bovine and human beta ARK1 in Sf9 cells and purified them to apparent homogeneity in milligram quantities. The Km-va...

journal_title：FEBS letters

authors： Söhlemann P,Hekman M,Buchen C,Elce JS,Lohse MJ

更新日期：1993-06-07 00:00:00

abstract：:Phosphorylated sites of calf thymus H2B histone were investigated with a catalytic fragment of 72 kDa protein-tyrosine kinase (p72syk). Three of five tyrosine residues in H2B histone can be phosphorylated by this kinase. In this analysis, H2B histone was thoroughly phosphorylated in vitro with [gamma-32P]ATP and the k...

journal_title：FEBS letters

authors： Sakai K,Tanaka Y,Asahi M,Shimomura R,Taniguchi T,Hashimoto E,Yamamura H

更新日期：1991-12-02 00:00:00

abstract：:Replacements of Asp-295 by Asn (D295N) and Glu (D295E) decreased the catalytic center activity of Leuconostoc mesenteroides sucrose phosphorylase to about 0.01% of the wild-type level (k(cat)=200s(-1)). Glucosylation and deglucosylation steps of D295N were affected uniformly, approximately 10(4.3)-fold, and independen...

journal_title：FEBS letters

authors： Mueller M,Nidetzky B

更新日期：2007-04-03 00:00:00

abstract：:Rats were infused intravenously with a bolus of mevalonolactone or cholesterol-rich chylomicron and microsomes prepared from the livers at times up to 2 h after the infusion. Acyl-CoA cholesterol acyltransferase (ACAT) activity was measured in the microsomes under conditions that define the activity state of the enzym...

journal_title：FEBS letters

authors： Suckling KE,Stange EF,Dietschy JM

更新日期：1983-08-08 00:00:00

abstract：:A cDNA clone encoding cholesterol side-chain cleavage cytochrome P450 (P450scc) was isolated from a rainbow trout ovarian follicle cDNA library. The cDNA contains an open reading frame of 1,542 nucleotides encoding a protein of 514 amino acids. The predicted amino acid sequence of trout P450scc shows 48% homology with...

journal_title：FEBS letters

authors： Takahashi M,Tanaka M,Sakai N,Adachi S,Miller WL,Nagahama Y

更新日期：1993-03-15 00:00:00

abstract：:The conformation change of Thermus thermophilus tRNA(1Ile) upon complex formation with T. thermophilus elongation factor Tu (EF-Tu) was studied by analysis of the circular dichroism (CD) bands at 315 nm (due to the 2-thioribothymidine residue in the T-loop) and at 295 nm (due to the core structure of tRNA). Formation ...

journal_title：FEBS letters

authors： Haruki M,Matsumoto R,Hara-Yokoyama M,Miyazawa T,Yokoyama S

更新日期：1990-04-24 00:00:00

abstract：:Granulocyte colony-stimulating factor (G-CSF) has been recently shown to induce the high-affinity Fc receptor for IgG (Fc(gammaRI)/CD64) in human polymorphonuclear neutrophils (PMN). To elucidate the molecular mechanisms whereby G-CSF exerts this effect, we examined whether the cytokine induces the binding of transcri...

journal_title：FEBS letters

authors： Bovolenta C,Gasperini S,Cassatella MA

更新日期：1996-05-20 00:00:00

abstract：:Interaction of the water oxidising manganese complex of photosystem II with the aqueous environment has been investigated using electron paramagnetic resonance spectroscopy and electron spin echo envelope modulation spectroscopy to detect interaction of [2H]methanol with the complex in the S2 state. The experiments sh...

journal_title：FEBS letters

authors： Evans MC,Gourovskaya K,Nugent JH

更新日期：1999-05-07 00:00:00

abstract：:Monoclonal antibodies (mAbs) against cell surface antigens and receptors are instrumental in defining specific membrane markers. mAbs GF 26.7.3 and MF 25.1 against human neutrophils modulated the activation mechanism of superoxide anion production induced by formyl-peptide and PMA in all subject. However, treatment wi...

journal_title：FEBS letters

authors： Traniello S,Spisani S,Gavioli R,Dovigo L,Baricordi RO,Sensi A,Damiani G

更新日期：1986-08-11 00:00:00

abstract：:Complexes between the urokinase-type plasminogen activator (uPA) and its type-2 inhibitor (PAI-2) are bound by a cell-surface receptor for uPA and rapidly cleaved into two fragments of 70 and 22 kDa. The 70-kDa fragment contains the active site of uPA and PAI-2, while the 22-kDa species was identified as the amino ter...

journal_title：FEBS letters

authors： Ragno P,Montuori N,Vassalli JD,Rossi G

更新日期：1993-06-01 00:00:00

abstract：:Hypohalous acids are poor one-electron oxidizing agents, such that reactions with hydrogen peroxide to yield radical species are not feasible. However, the oxidation of superoxide by hypohalous acids can be a source of hydroxyl or haline radicals. The oxidation of nitrogen monoxide by hypochlorous acid is favourable, ...

journal_title：FEBS letters

authors： Koppenol WH

更新日期：1994-06-20 00:00:00

abstract：:Living isolated mitochondria incubated with iron(II) show a major alteration in mitochondrial DNA (mtDNA) conformational forms as assessed by Southern blot analysis of undigested mtDNA. In the presence of iron(II), form I is transformed into form III in a dose-dependent manner. This alteration in mtDNA conformation sh...

journal_title：FEBS letters

authors： Asín J,Pérez-Martos A,Fernández-Silva P,Montoya J,Andreu AL

更新日期：2000-09-01 00:00:00

abstract：:Neurofibrillary tangles formed by abnormally aggregated tau protein are a histopathological feature of tauopathies. A tau aggregation inhibitor is a potential therapeutic agent for tauopathies. In this study, we prepared a monoclonal antibody for tau, monoclonal antibody to tau protein (Tau2r3), using as epitope the 2...

journal_title：FEBS letters

authors： Tsuchida T,Susa K,Kibiki T,Tsuchiya T,Miyamoto K,In Y,Minoura K,Taniguchi T,Ishida T,Tomoo K

更新日期：2020-04-13 00:00:00

abstract：:Anandamide loses its cannabimimetic activities upon hydrolysis to arachidonic acid and ethanolamine. So far the anandamide hydrolyzing activity widely distributed in mammalian organs has been attributed exclusively to an enzyme referred to as anandamide amidohydrolase with an optimum pH around 9. We found another enzy...

journal_title：FEBS letters

authors： Ueda N,Yamanaka K,Terasawa Y,Yamamoto S

更新日期：1999-07-09 00:00:00

abstract：:The actin-depolymerising factor (ADF)/cofilin group of proteins are stimulus-responsive actin-severing proteins, members of which are regulated by reversible phosphorylation. The phosphorylation site on the maize ADF, ZmADF3, is Ser-6 but the kinase responsible is unknown [Smertenko et al., Plant J. 14 (1998) 187-193]...

journal_title：FEBS letters

authors： Allwood EG,Smertenko AP,Hussey PJ

更新日期：2001-06-15 00:00:00

abstract：:Mesothelial cells, which cover the surface of visceral organs and serous cavities in mammals, play a crucial role in preventing adhesion. We previously reported that primary mesothelial progenitor cells (MPCs) can not only prevent postoperative adhesion but also promote liver regeneration after hepatectomy. Induced pl...

journal_title：FEBS letters

authors： Inagaki NF,Inagaki FF,Kokudo N,Miyajima A

更新日期：2019-02-01 00:00:00

abstract：:The effect of caffeine on catecholamine secretion and intracellular free Ca2+ concentration [( Ca2+]i) in bovine adrenal chromaffin cells was examined using single fura-2-loaded cells and cell populations. In cell populations caffeine elicited a large (approximately 200 nM) transient rise in [Ca2+]i that was independe...

journal_title：FEBS letters

authors： Cheek TR,O'Sullivan AJ,Moreton RB,Berridge MJ,Burgoyne RD

更新日期：1990-06-18 00:00:00

abstract：:The NusB protein is involved in transcriptional regulation in bacteriophage lambda. NusB binds to the RNA form of the nut site and along with N, NusA, NusE and NusG, stabilizes the RNA polymerase transcription complex and allows stable, persistent antitermination. NusB contains a 10 residue Arg-rich RNA-binding motif ...

journal_title：FEBS letters

authors： Altieri AS,Mazzulla MJ,Zhou H,Costantino N,Court DL,Byrd RA

更新日期：1997-09-29 00:00:00

abstract：:The last enzyme (LytB) of the methylerythritol phosphate pathway for isoprenoid biosynthesis catalyzes the reduction of (E)-4-hydroxy-3-methylbut-2-enyl diphosphate into isopentenyl diphosphate and dimethylallyl diphosphate. This enzyme possesses a dioxygen-sensitive [4Fe-4S] cluster. This prosthetic group was charact...

journal_title：FEBS letters

authors： Wolff M,Seemann M,Tse Sum Bui B,Frapart Y,Tritsch D,Garcia Estrabot A,Rodríguez-Concepción M,Boronat A,Marquet A,Rohmer M

更新日期：2003-04-24 00:00:00

abstract：:In the bovine ovary there is a delay of 4-6 days between the observed maximum of oxytocin mRNA and the peak in the luteal levels of oxytocin nonapeptide. This implies a maturation process involving components of the post-translational processing pathway. In situ hybridization shows the oxytocin gene to be transcribed ...

journal_title：FEBS letters

authors： Fehr S,Ivell R,Koll R,Schams D,Fields M,Richter D

更新日期：1987-01-01 00:00:00

abstract：:BSP-30K is a major acidic glycoprotein of bovine seminal plasma. It displays heparin-, gelatin-, and phospholipid-binding activities. BSP-30K binds to spermatozoa upon ejaculation and is thought to play a role in sperm capacitation. We have determined its amino acid sequence, disulfide bonds, and 0-glycosylation sites...

journal_title：FEBS letters

authors： Calvete JJ,Mann K,Sanz L,Raida M,Töpfer-Petersen E

更新日期：1996-12-09 00:00:00

abstract：:The conformation in solution of the biantennary complex type oligosaccharide unit derived from human serotransferrin has been investigated using 1H-1H Nuclear Overhauser Effect (NOE) measurements at 300 MHz. From quantitation of the NOE, the alpha(1-3) antenna is shown to exist in a preferred solution conformation wit...

journal_title：FEBS letters

authors： Homans SW,Dwek RA,Fernandes DL,Rademacher TW

更新日期：1982-12-27 00:00:00

abstract：:Fructose 2,6-bisphosphate has been claimed to be both a substrate analogue and an allosteric inhibitor of fructose-1,6-bisphosphatase. The results reported here show that fructose 2,6-bisphosphate can be both an inhibitor and an activator of the enzyme, depending on the substrate concentration. This biphasic behaviour...

journal_title：FEBS letters

authors： Corredor C,Boscá L,Sols A

更新日期：1984-02-27 00:00:00

abstract：:The fluorescent calcium indicator 'quin2' was used to demonstrate changes in cytoplasmic calcium concentrations in bovine anterior pituitary cells. The basal calcium concentration was 0.21 +/- 0.02 microM (mean of 4 cell preparations). Thyroliberin (TRH) (10(-10) - 10(-6) M) rapidly and at the higher concentrations tr...

journal_title：FEBS letters

authors： Schofield JG

更新日期：1983-08-08 00:00:00

abstract：:The MDM2 oncoprotein is overexpressed in many human tumors and cancers. MDM2 functions as an E3 ligase for p53 and for itself. MDM2 also interacts with the retinoblastoma protein (RB) and the transcription factor E2F1 to promote cell cycle S-phase entry. Here, we report that MDM2 protein expression is cell cycle-regul...

journal_title：FEBS letters

authors： Gu L,Ying H,Zheng H,Murray SA,Xiao ZX

更新日期：2003-06-05 00:00:00