Conformational fluctuations in anthrax protective antigen: a possible role of calcium in the folding pathway of the protein.

abstract：:Originally identified as multicopy suppressor of a lethal growth arrest caused by expression of a tumour mutant cDNA of p53 in fission yeast the tms1 gene product was found to form stable complexes with p53 in yeast. By using purified recombinant proteins multimeric complexes of tms1 and p53 could be demonstrated and ...

journal_title：FEBS letters

authors： Wagner P,Fuchs A,Prowald A,Montenarh M,Nastainczyk W

更新日期：1995-12-18 00:00:00

abstract：:Translocation of folded proteins by the Tat system of Escherichia coli is believed to rely on the presence of phosphatidylethanolamine (PE) and the negatively charged phospholipids cardiolipin (CL) and phosphatidylglycerol (PG). Here, we show that while PE is indeed essential for activity, the Tat system is fully func...

journal_title：FEBS letters

authors： Rathmann C,Schlösser AS,Schiller J,Bogdanov M,Brüser T

更新日期：2017-09-01 00:00:00

abstract：:The cDNA coding for mature human alpha 1-proteinase inhibitor (alpha 1-PI) has been inserted into a variety of yeast expression vectors. Yeast cells transformed with these plasmids were then assayed for the production of mature, unglycosylated alpha 1-PI. The production level is optimal when the recombinant plasmid ca...

journal_title：FEBS letters

authors： Hoylaerts M,Weyens A,Bollen A,Harford N,Cabezón T

更新日期：1986-08-11 00:00:00

abstract：:The 5.8S and 28S rRNA sequences of the oomycete Phytophthora megasperma were determined in order to study the secondary structure of these molecules and to assess the phylogenetic position of the oomycetes among the eukaryotes. Preliminary results point to an affiliation between the oomycetes, dinoflagellates and cili...

journal_title：FEBS letters

authors： Van der Auwera G,Chapelle S,De Wachter R

更新日期：1994-01-31 00:00:00

abstract：:Six charged amino acid residues located in the ectodomain of the full-length type I transforming growth factor (TGF)-beta receptor were individually mutated to alanine. Mutation of residues D47, D98, K102 and E104 resulted in functionally impaired receptors as demonstrated by a marked decrease in ligand-dependent sign...

journal_title：FEBS letters

authors： Guimond A,Sulea T,Zwaagstra JC,Ekiel I,O'Connor-McCourt MD

更新日期：2002-02-27 00:00:00

abstract：:The adipose-derived plasma protein, adiponectin (APN), has various protective effects on cardiovascular diseases. In this study, we show that endogenous APN is required for full cyclooxygenase-2 (COX-2) induction by ischemia-reperfusion injury in the heart in vivo. In rat neonatal cardiac myocytes, APN-induced COX-2 e...

journal_title：FEBS letters

authors： Ikeda Y,Ohashi K,Shibata R,Pimentel DR,Kihara S,Ouchi N,Walsh K

更新日期：2008-04-02 00:00:00

abstract：:A functional rat heart Na(+)-Ca2+ exchanger gene has been obtained by splicing and ligating two partially overlapping clones isolated from a rat heart lambda ZAP cDNA library. The deduced primary structure of the protein encoded by the open reading frame corresponds to 971 amino acids, that can be organized into 12 tr...

journal_title：FEBS letters

authors： Low W,Kasir J,Rahamimoff H

更新日期：1993-01-18 00:00:00

abstract：:Bleomycin-reactive iron was detected in the sera of six out of nine adults undergoing intensive chemotherapy for acute non-lymphocytic leukemia. In these individuals the corresponding transferrin saturation ranged from 96% to 113% and the serum ferritin from 775 to 9975 micrograms/l. Nontransferrin-bound iron has been...

journal_title：FEBS letters

authors： Gordeuk VR,Brittenham GM

更新日期：1992-08-10 00:00:00

abstract：:Methane monooxygenase (MMO) catalyses the biological transformation of methane to methanol at a binuclear iron site. Guided by the three-dimensional structure of the R2 protein of E. coli ribonucleotide reductase (RNR), we have aligned the sequences of two different MMOs with the sequences of the iron coordinating fou...

journal_title：FEBS letters

authors： Nordlund P,Dalton H,Eklund H

更新日期：1992-08-03 00:00:00

abstract：:Glucosamine-6-phosphate (GlcN6P) N-acetyltransferase 1 (GNA1) is a key enzyme in the pathway toward biosynthesis of UDP-N-acetylglucosamine, an important donor substrate for N-linked glycosylation. GNA1 catalyzes the formation of N-acetylglucosamine-6-phosphate (GlcNAc6P) from acetyl-CoA (AcCoA) and the acceptor subst...

journal_title：FEBS letters

authors： Wang J,Liu X,Liang YH,Li LF,Su XD

更新日期：2008-09-03 00:00:00

abstract：:On incubation of 7-day-old wheat seedlings in the presence of [3H]thymidine, the radioactivity incorporated into coleoptile DNA is found to be localized mainly (greater than 95%) in the fraction of heavy mitochondrial DNA (H-mt DNA; rho = 1.716 gm/cm3). Upon long (48-72 h) incubation of cut-off seedlings in water, the...

journal_title：FEBS letters

authors： Kirnos MD,Alexandrushkina NI,Zagorskaya GYa,Kireev II,Vanyushin BF

更新日期：1992-02-24 00:00:00

abstract：:The structure determination of apotransketolase and the comparison of its three-dimensional structure with that of the holoenzyme has revealed that no large conformational changes are associated with cofactor binding. Two loops at the active site are flexible in the apoenzyme which enables ThDP to reach its binding si...

journal_title：FEBS letters

authors： Sundström M,Lindqvist Y,Schneider G

更新日期：1992-11-30 00:00:00

abstract：:Zinc metallopeptidases that contain the His-Glu-Xaa-Xaa-His (HEXXH) motif generally have a third ligand of the metal ion that may be either a Glu residue (in clan MA) or a His residue (in clan MB) (Rawlings and Barrett (1995) Methods Enzymol. 248, 183-228). Thimet oligopeptidase has not yet been assigned to either cla...

journal_title：FEBS letters

authors： Chen JM,Stevens RA,Wray PW,Rawlings ND,Barrett AJ

更新日期：1998-09-11 00:00:00

abstract：:The Wnt/beta-catenin signalling pathway is evolutionarily conserved across many species and plays important roles during embryogenesis. The Lee-Heinrich model (to recognize the contributions of R. Heinrich we will refer to the work proposed by Lee et al. [Lee, E., Salic, A., Krüger, R., Heinrich, R., Kirschner, M.W. (...

journal_title：FEBS letters

authors： Wawra C,Kühl M,Kestler HA

更新日期：2007-08-21 00:00:00

abstract：:The addition of 5-azacytidine to erythroleukemic cells which were induced to differentiate with DMSO or BA altered the expression of the hemoglobins. After the addition of 5-azacytidine there was an increase in hemoglobin synthesis especially in the embryonic E2 band. The beta-globin increased in synthesis after 5-aza...

journal_title：FEBS letters

authors： Zucker RM,Decal DL,Whittington KB

更新日期：1983-10-17 00:00:00

abstract：:We investigated 38 cases of B-cell chronic lymphocytic leukemia (B-CLL) for the presence of non-productive rearrangements in the S(mu) regions and defined for the first time the molecular nature of these rearrangements. Southern blot analysis revealed S(mu) region rearrangements in 13 cases (34%) and polymerase chain ...

journal_title：FEBS letters

authors： Nardini E,Rizzi S,Capello D,Vitolo U,Gaidano G,Menard S,Balsari A

更新日期：2002-05-08 00:00:00

abstract：:Two-photon scanning laser and confocal microscopies were used to image metabolic dynamics of single or cell populations of Saccharomyces cerevisiae strain 28033. Autofluorescence of reduced nicotinamide nucleotides, and mitochondrial membrane potential (DeltaPsim), were simultaneously monitored. Spontaneous, large-sca...

journal_title：FEBS letters

authors： Aon MA,Cortassa S,Lemar KM,Hayes AJ,Lloyd D

更新日期：2007-01-09 00:00:00

abstract：:The [Cu(I)-Cu(II)] half-met form of the dinuclear copper site of tyrosinase has been probed by continuous wave electron paramagnetic resonance (EPR) and hyperfine sublevel correlation (HYSCORE) spectroscopy in the presence and absence of inhibitors. In all cases the EPR spectrum is indicative of a d(x(2)-y(2)) ground ...

journal_title：FEBS letters

authors： van Gastel M,Bubacco L,Groenen EJ,Vijgenboom E,Canters GW

更新日期：2000-06-02 00:00:00

abstract：:Plasmids for the high-level overproduction of wild-type, and C- and N-terminal His-tagged MurG N-acetylglucosaminyl transferase from Escherichia coli were constructed. In complementation tests the three forms were active in vivo. After IPTG induction, growth, spheroplast formation and lysis, overproduced MurG proteins...

journal_title：FEBS letters

authors： Crouvoisier M,Mengin-Lecreulx D,van Heijenoort J

更新日期：1999-04-23 00:00:00

abstract：:Parathyroid hormone (PTH) secretion is stimulated by low extracellular calcium (Ca2+) in association with a reduction in cyosolic Ca2+, indicating that this cell type does not conform to classical models of stimulus-secretion coupling. We used the phorbol ester TPA (12-O-tetradecanoyl phorbol 13-acetate), which direct...

journal_title：FEBS letters

authors： Brown EM,Redgrave J,Thatcher J

更新日期：1984-09-17 00:00:00

abstract：:PDZ domains are thought to act as protein-binding modules mediating the clustering of membrane and membrane-associated proteins. The INAD protein has been shown to interact via a PDZ domain with the calcium channel TRP which contributes to capacitative calcium entry into Drosophila photoreceptor cells. We have cloned ...

journal_title：FEBS letters

authors： Philipp S,Flockerzi V

更新日期：1997-08-18 00:00:00

abstract：:Cyclin B is a central regulator of transition from the G(2) phase of the cell cycle to mitosis. In mammalian cells two B-type cyclins have been characterised, cyclin B1 and B2. Both are expressed with a maximum in G(2) and their synthesis is mainly regulated on the transcriptional level. We show that a single cell cyc...

journal_title：FEBS letters

authors： Wasner M,Tschöp K,Spiesbach K,Haugwitz U,Johne C,Mössner J,Mantovani R,Engeland K

更新日期：2003-02-11 00:00:00

abstract：:Hemocyanin is present as 2 subunits in the hemolymph of Penaeus vannamei. Isolated from a hepatopancreas cDNA library of this penaeid shrimp, the cDNA chain (2095 bp) corresponds to a full length hemocyanin messenger as determined by Northern hybridization, with a short 5' untranslated region (17 bp), an open reading ...

journal_title：FEBS letters

authors： Sellos D,Lemoine S,Van Wormhoudt A

更新日期：1997-04-28 00:00:00

abstract：:In this study we identify the molecules involved in the MAPK signal transduction pathway (Ras, Raf-1, Mek, Mek-P and MAPK) in highly purified endosomal fractions isolated from rat liver. Biochemical analysis shows that only the early-sorting endocytic compartment contains activated Raf-1 and Mek. Finally, the exogenou...

journal_title：FEBS letters

authors： Pol A,Calvo M,Enrich C

更新日期：1998-12-11 00:00:00

abstract：:Calf chymosin molecules exist in the two alternative structural forms: the first one has S1 and S3 binding pockets occluded by its own Tyr77 residue (the self-inhibited form); the second has these pockets free for a substrate binding (the active form). The preliminary incubation of the enzyme with a pentapeptide corre...

journal_title：FEBS letters

authors： Gustchina E,Rumsh L,Ginodman L,Majer P,Andreeva N

更新日期：1996-01-22 00:00:00

abstract：:The primary structure of nuclear factor-I (CTF/NF-I), a eukaryotic regulatory DNA-binding protein involved in both DNA replication and gene transcription, and the secondary structure predictable from it, are compared here with those of a number of prokaryotic acetylases. Hydropathy and Chou-Fasman analyses reveal that...

journal_title：FEBS letters

authors： Oikarinen J,Mannermaa RM

更新日期：1990-10-29 00:00:00

abstract：:Proteins involved in protein translocation across the membrane of the endoplasmic reticulum assemble into different oligomeric complexes depending on their state of function. To analyse such membrane protein complexes we fractionated proteins of mammalian rough microsomes and analysed them using blue native PAGE and i...

journal_title：FEBS letters

authors： Wang L,Dobberstein B

更新日期：1999-09-03 00:00:00

abstract：:Helical filaments of bacterial flagella are built up by a self-assembly process from thousands of flagellin subunits. To clarify how the disordered terminal regions of flagellin interact upon filament formation, polymerization ability of various terminally truncated fragments was investigated. Fragments deprived of 19...

journal_title：FEBS letters

authors： Gugolya Z,Muskotál A,Sebestyén A,Diószeghy Z,Vonderviszt F

更新日期：2003-01-30 00:00:00

abstract：:A characteristic of the antioxidant, probucol, is its inability to inhibit apolipoprotein B fragmentation in low density lipoprotein (LDL), despite a pronounced ability to inhibit lipid oxidation on relatively lengthy exposure to Cu(II). Here we show that a short exposure of LDL to hydrogen peroxide and Cu(II) leads t...

journal_title：FEBS letters

authors： Hunt JV,Bailey JR,Schultz DL,McKay AG,Mitchinson MJ

更新日期：1994-08-08 00:00:00

abstract：:Selenium depletion of H4 hepatoma cells reduced cytosolic glutathione peroxidase (cGSH-Px) mRNA abundance but had no effect on phospholipid hydroperoxide glutathione peroxidase (PHGSH-Px) mRNA abundance. Actinomycin D chase experiments showed that selenium depletion had no effect on the stability of PHGSH-Px mRNA but ...

journal_title：FEBS letters

authors： Bermano G,Arthur JR,Hesketh JE

更新日期：1996-06-03 00:00:00