Abstract:
:Isolated rat islets of Langerhans permeabilised by high-voltage discharge secreted insulin in response to elevations in Ca2+ over the range 100 nM to 10 microM Ca2+. The phorbol ester, 12-O-tetradecanoylphorbol 13-acetate (TPA), had no effects on insulin secretion in the absence of Ca2+. In the presence of Ca2+ concentrations of greater than 10 nM, TPA produced dose-related shifts in the Ca2+-activation curve to lower Ca2+ concentrations, together with marked increases in the maximum secretory response to Ca2+. These results suggest that, in islets, the activation of protein kinase C is important in modulating both the sensitivity of the exocytotic mechanism to intracellular Ca2+, and the magnitude of the insulin secretory response.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Jones PM,Stutchfield J,Howell SLdoi
10.1016/0014-5793(85)81002-4subject
Has Abstractpub_date
1985-10-21 00:00:00pages
102-6issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(85)81002-4journal_volume
191pub_type
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