Abstract:
:Peroxisome proliferator-activated receptor gamma (PPARgamma) ligands have been shown to inhibit the effects of proinflammatory cytokines such as interleukin-1beta (IL-1beta). This cytokine plays a key role in articular pathophysiologies by inducing the production of inflammatory mediators such as nitric oxide (NO) and prostaglandin E(2) (PGE(2)). We previously demonstrated that 15d-PGJ(2) was more potent than troglitazone to counteract IL-1beta effects on chondrocytes. Here, we studied the action of 15d-PGJ(2) on intracellular targets in nuclear factor-kappaB (NF-kappaB) signalling pathway in IL-1beta treated rat chondrocytes. We found that 15d-PGJ(2) decreased inhibitor kappaBalpha (IkappaBalpha) degradation but not its phosphorylation by specifically inhibiting IkappaB kinase beta (IKKbeta), but not IKKalpha, enzymatic activity. We further evaluated the involvement of PPARgamma in the anti-inflammatory action of its ligands. In chondrocytes overexpressing functional PPARgamma protein, 15d-PGJ(2) pre-treatment inhibited inducible NO synthase and COX-2 mRNA expression, nitrite and PGE(2) production, p65 translocation and NF-kappaB activation. Troglitazone or rosiglitazone pre-treatment had no effect. 15d-PGJ(2) exhibited the same effect in chondrocytes overexpressing mutated PPARgamma protein. These results suggest that 15d-PGJ(2) exerts its anti-inflammatory effect in rat chondrocytes by a PPARgamma-independent mechanism, which can be conferred to a partial inhibition of IkappaBalpha degradation.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Boyault S,Bianchi A,Moulin D,Morin S,Francois M,Netter P,Terlain B,Bordji Kdoi
10.1016/j.febslet.2004.06.090keywords:
subject
Has Abstractpub_date
2004-08-13 00:00:00pages
33-40issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579304008609journal_volume
572pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::To determine the amino acid residues required for the signal-transducing activity of the human c-Ha-Ras protein, we introduced point mutations at residues 45-54 near the 'effector region' (residues 32-40). We transfected PC12 cells with these mutant genes and also micro-injected the mutant proteins, bound with an unhy...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80665-p
更新日期:1991-12-09 00:00:00
abstract::DOCK and Affinity studies were carried out to study the binding of D- and L-penicillamine to the transactivator protein (tat) of human immunodeficiency virus type 1 (HIV-1). These studies reveal a selective binding of D-penicillamine to the cysteine-rich region covering amino acid residues 20-38 of the tat protein. A ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02468-7
更新日期:2002-04-10 00:00:00
abstract::The bacteriophytochrome Agp1 was reconstituted with a locked 5Zs-biliverdin in which the C(4)=C(5) and C(5)-C(6) bonds of the methine bridge between rings A and B are fixed in the Z and syn configuration/conformation, respectively. In Agp1-5Zs the photoconversion proceeds via the Lumi-R intermediate to Meta-R(A), but ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.10.043
更新日期:2007-11-27 00:00:00
abstract::Heparin-binding epidermal growth factor-like growth factor (HB-EGF), a potent mitogen and migration factor for vascular smooth muscle cells (SMC), promoted neovascularization in vivo in the rabbit cornea. MRI demonstrated quantitatively the angiogenic effect of HB-EGF when introduced subcutaneously into nude mice. HB-...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00283-x
更新日期:1998-04-03 00:00:00
abstract::DNA polymerase alpha-primase is a heterotetrameric complex essential for simian vacuolating virus 40 (SV40) DNA replication. We show that the C-terminal 67 amino acid residues of the human p180 subunit are essential for SV40 DNA replication as they are required for binding of the p68 subunit and play a role in the int...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03197-6
更新日期:2002-09-11 00:00:00
abstract::Gamma-aminobutyric acid type A (GABAA) receptor beta1 (gabrb1), a subunit of GABAA receptors involved in inhibitory effects on neurotransmission, was found to associate with the formation of protease-resistant prion protein in prion-infected neuroblastoma cells. Silencing of gabrb1 gene expression significantly decrea...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.02.029
更新日期:2010-03-19 00:00:00
abstract::The activity of the Ca-pumping ATPase of cardiac sarcoplasmic reticulum (SR) is controlled by the phosphorylation of the intrinsic regulatory protein phospholamban (PLB), which affects both the apparent Km(Ca) and the Vmax of the transport process. We have investigated the correlation between phosphorylation of PLB an...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81201-3
更新日期:1989-02-13 00:00:00
abstract::The functional characteristics of rat organic anion transporter OAT1 were investigated using Xenopus laevis oocytes. Uptake of p-aminohippurate (PAH) by the oocytes expressing OAT1 was markedly inhibited by glutarate, alpha-ketoglutarate and probenecid, moderately inhibited by folate and methotrexate, but not inhibite...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01328-3
更新日期:1998-11-06 00:00:00
abstract::Latent transforming growth factor-beta 1 binding protein (LTBP) is a constituent of the latent high molecular weight complex of TGF-beta 1 in human platelets. In the present communication, we show that LTBP binds Ca2+ in its free form as well as in the latent TGF-beta 1 complex. The binding of Ca2+ induces a structura...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80079-a
更新日期:1993-04-05 00:00:00
abstract::Activation-induced cytidine deaminase (AID), a member of the APOBEC family that induces antibody diversification, has been shown to inhibit the replication of hepatitis B virus, Kaposi's sarcoma-associated herpesvirus, and retro-transposons. However, whether AID can inhibit human immunodeficiency virus 1 (HIV-1) repli...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12954
更新日期:2018-01-01 00:00:00
abstract::Previously determined protein structures have been analysed, in order to find folding motifs similar to that proposed by NMR spectroscopy, for the N-terminal, 14 kDa fragment of the Ada protein. The analyses reveal only limited similarities with the NMR-derived structural data and strongly suggest that this region of ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81352-z
更新日期:1993-06-01 00:00:00
abstract::Using a BAL31 exonuclease assay to determine the sites of 4,5',8-trimethylpsoralen photocrosslinking in DNA we have shown that 5'-TA sites which are accessible to psoralen DNA interstrand photocrosslinking in naked DNA become inaccessible when protein, in casu, lambda-repressor E. coli or RNA polymerase are bound at t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80800-7
更新日期:1988-02-29 00:00:00
abstract::Nitric oxide (.NO) synthase (NOS) activity in subcellular fractions from cultured endothelial cells (EC) and lipopolysaccharide-activated J774.2 monocyte/macrophages was investigated by monitoring the .NO-mediated increase in intracellular cyclic GMP in LLC-PK1 pig kidney epithelial cells. The constitutive NOS in EC (...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81434-a
更新日期:1991-12-09 00:00:00
abstract::Hemoglobin-binding proteins are necessary for pathogens to obtain iron from Hb. Entamoeba histolytica can grow using Hb as source of iron, but the underlying mechanism has not previously been established. In this work, we identified a 45 kDa Hb-binding protein of E. histolytica, which we named Ehhmbp45. In silico anal...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.06.057
更新日期:2008-08-06 00:00:00
abstract::To clarify the function of osteopontin in osteoblast differentiation, we have examined the signal transduction pathway in an osteoblastic cell line (UMR106-6) bound to osteopontin, fibronectin, vitronectin and collagen type I surfaces. This was done by investigating the production and autophosphorylation of focal adhe...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01498-1
更新日期:1997-12-22 00:00:00
abstract::Apicomplexan parasites contain a vestigial plastid called apicoplast which has been suggested to be a site of [Fe-S] cluster biogenesis. Here we report the cloning of lipoic acid synthase (LipA) from Toxoplasma gondii, a well known [Fe-S] protein. It is able to complement a LipA-deficient Escherichia coli strain, clea...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00673-2
更新日期:2003-07-17 00:00:00
abstract::Over 400 human proteases documented in secondary databases can already be delineated in genomic sequence. A Genome Ontology annotation of 30585 sequences in the provisional human proteome set recognises 498 proteases, i.e. 1.6%. Homology searches against finished sequence and comparisons between mouse and zebrafish ar...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(01)02490-5
更新日期:2001-06-08 00:00:00
abstract::A recombinant expression system was developed to analyse sequence determinants involved in O-glycosylation of proteins in mycobacteria. By expressing peptide sequences corresponding to known glycosylation sites within a chimeric lipoprotein construct, amino acids flanking modified threonine residues were found to have...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01553-2
更新日期:2000-05-19 00:00:00
abstract::Proteases not only play a fundamental role in numerous physiological processes, but are also involved in several human diseases including Alzheimer's disease (AD). A key protease implicated in AD is the so far unidentified gamma-secretase, which cleaves the membrane-bound beta-amyloid precursor protein (betaAPP) at th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01627-0
更新日期:1999-12-17 00:00:00
abstract::The carbohydrate binding properties of jacalin lectin were examined using RAF9 cell-derived D-[6-3H]glucosamine-radiolabeled total glycopeptides containing N-linked and O-linked oligosaccharides. The binding of N-linked glycopeptides to jacalin was abolished by treatment of alpha-galactosidase whereas O-linked glycope...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01539-1
更新日期:1998-01-09 00:00:00
abstract::Here, we report the volume and enthalpy changes accompanying CO photodissociation from the mixed valence form of cytochrome bo3 oxidase from Escherichia coli. The results of photoacoustic calorimetry indicate two kinetic phases with distinct volume and enthalpy changes accompanying CO photodissociation from heme o3 an...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.04.055
更新日期:2005-06-06 00:00:00
abstract::The nervous and immune systems have similar functional characteristics. Both have an intricate network of synaptic connections and an exquisite communication system that enable intercellular signal transduction. Although semaphorins were originally identified as guidance cues in neural development, accumulating eviden...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2011.03.033
更新日期:2011-12-01 00:00:00
abstract::Highly purified mitochondria from potato (Solanum tuberosum L. cv. Bintje) tubers were subfractionated into a matrix fraction, an inner membrane fraction and an outer membrane fraction with minimal cross-contamination. When the matrix and inner membrane fractions were incubated with [gamma-32P]ATP only one and three p...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80835-i
更新日期:1993-12-27 00:00:00
abstract::The RNA helicase Rok1 plays a role in rRNA processing and in control of cell cycle progression in Saccharomyces cerevisiae. We identified two upstream open reading frames (uORFs) within the ROK1 5' untranslated region, which inhibited Rok1 translation. Mutating uATG to uAAG or generation of a premature stop codon in t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.10.019
更新日期:2010-11-19 00:00:00
abstract::The effect of neuropeptide Y (NPY) on cytosolic free Ca2+ concentration ([Ca2+]i) was studied in cultured smooth muscle cells from porcine aorta (PASMC) and compared with the effect of bradykinin (BK) and angiotensin II (ATII) on [Ca2+]i. All peptides induced dose-dependent and transient rises in [Ca2+]i which were no...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81499-1
更新日期:1989-12-18 00:00:00
abstract::Adenosine kinase is one of the enzymes potentially responsible for the formation of cytokinin nucleotides in plants. Using a zeatin affinity column a 40 kDa protein was isolated from tobacco Bright Yellow 2 (TBY-2) and identified by mass spectrometry as adenosine kinase. The ligand interaction reported here can be dis...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03750-x
更新日期:2003-01-02 00:00:00
abstract::It is increasingly clear that microRNAs play a crucial role in tumorigenesis. Recently, emerging evidence suggested that miR-26a is aberrantly expressed in tumor tissues. In our study, frequent down-regulation of miR-26a was observed in 10 human bladder cancer tissues. Forced expression of miR-26a in the bladder cance...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.06.021
更新日期:2013-08-02 00:00:00
abstract::Smad ubiquitination regulatory factor 2 (Smurf2), a ubiquitin ligase for Smads, plays critical roles in the regulation of transforming growth factor-beta (TGF-beta)-Smad signaling via ubiquitin-dependent degradation of Smad2 and Smad7. We found that TGF-beta stimulates Smurf2 expression. TGF-beta activated the Smurf2 ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.03.069
更新日期:2005-05-09 00:00:00
abstract::The temperature dependence of the rate constants of the univalent redox steps YzoxSi----YzSi + 1 (i = 0,1,2) and YzoxS3----(YzS4)----YzSo + O2 in the water oxidase was investigated by measuring time resolved absorption changes at 355 nm induced by a laser flash train in dark adapted PS II membrane fragments from spina...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80092-u
更新日期:1992-03-24 00:00:00
abstract::Creatine kinases (CK) play a prominent role in cell energy distribution through an energy shuttle between mitochondria and other organelles. Human brain CK was cloned and overexpressed in COS-7 cells. We then deleted His-65 and/or Pro-66 situated near the center of a flexible loop as shown by X-ray crystallography on ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01614-8
更新日期:2000-06-09 00:00:00