Abstract:
:The functional characteristics of rat organic anion transporter OAT1 were investigated using Xenopus laevis oocytes. Uptake of p-aminohippurate (PAH) by the oocytes expressing OAT1 was markedly inhibited by glutarate, alpha-ketoglutarate and probenecid, moderately inhibited by folate and methotrexate, but not inhibited by taurocholate or tetraethylammonium. Methotrexate and folate were transported by OAT1, but probenecid, a typical inhibitor of organic anion transporter, was not transported. Inhibition of PAH uptake by aliphatic dicarboxylates with various alkyl chain lengths was maximal at 5 (glutarate) and 6 (adipate) carbon atoms. OAT1-mediated PAH uptake was markedly inhibited by phorbol 12-myristate 13-acetate (PMA), phorbol 12,13-dibutyrate and mezerein, but not by 4alpha-phorbol 12,13-didecanoate. The inhibitory effect of PMA was attenuated in the presence of staurosporine, suggesting that OAT1 is regulated by protein kinase C. These results suggest that the substrate recognition of OAT1 is comparable to that of renal basolateral organic anion transporter, and the transport activity is regulated by protein kinase C.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Uwai Y,Okuda M,Takami K,Hashimoto Y,Inui Kdoi
10.1016/s0014-5793(98)01328-3subject
Has Abstractpub_date
1998-11-06 00:00:00pages
321-4issue
3eissn
0014-5793issn
1873-3468pii
S0014579398013283journal_volume
438pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Studies were undertaken to further elucidate the mechanism(s) by which bradykinin-dependent phosphoinositide metabolism takes place in neuroblastoma X glioma hybrid NG108-15 cells [(1984) J. Biol. Chem. 259, 10201-10207] using [3H]inositol-labelled cells. Bradykinin produced net increases in the level of [3H]inositol ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80301-x
更新日期:1985-02-25 00:00:00
abstract::The four-step caffeine biosynthetic pathway includes three methylation steps that utilise S-adenosyl-L-methionine (SAM) as the methyl donor. In the process SAM is converted to S-adenosyl-L-homocysteine (SAH) which in turn is hydrolysed to L-homocysteine and adenosine. Significant amounts of radioactivity from [methyl-...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02512-1
更新日期:2001-06-15 00:00:00
abstract::Vacuolar membrane H(+)-translocating pyrophosphatase (H(+)-PPase) was purified from pumpkin seedlings. Its enzymatic properties including molecular size of constituting polypeptide (75 kDa) were very similar to those of mung bean H(+)-PPase [(1989) J. Biol. Chem. 264, 20068-20073]. The native, functional molecular siz...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81254-6
更新日期:1991-09-23 00:00:00
abstract::The membrane-membrane fusion during fertilization of oocyte by spermatozoa is believed to be mainly mediated by so called "fusion proteins". In the present study we have tried to demonstrate that beside the proteins, lipid components of membrane may play an important role in fusion of oocyte with spermatozoa. Conventi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.03.015
更新日期:2006-04-17 00:00:00
abstract::The heat shock factor (HSF1) is the central regulator of the heat stress (hs) response and is required for stimulating the transcription of the hs genes and consequently the expression of heat shock proteins. To promote the polymerase II-dependent transcription of the hs genes, HSF has to communicate with the basal tr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01152-1
更新日期:1998-10-09 00:00:00
abstract::Elongation factor Tu (EF-Tu) is the most abundant protein in prokaryotic cells. Its general function in protein biosynthesis is well established. It is a member of the large family of G-proteins, all of which bind guanosine phosphates (GDP or GTP) as cofactors. In its active GTP bound state EF-Tu binds aminoacylated t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01254-7
更新日期:1994-12-19 00:00:00
abstract::Incorporation of dioleoyl N-(monomethoxy polyethyleneglycol succinyl)phosphatidylethanolamine (PEG-PE) into large unilamellar liposomes composed of egg phosphatidylcholine:cholesterol (1:1) does not significantly increase the content leakage when the liposomes are exposed to 90% human serum at 37 degrees C, yet the li...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81016-h
更新日期:1990-07-30 00:00:00
abstract::Insulin receptors (IRs) that are truncated at the end of the ectodomain form dimers that bind insulin with different characteristics to wild type receptors. These soluble IRs have lowered affinity for insulin compared with full-length IR, and exhibit linear Scatchard plots in contrast to the curvilinear plots obtained...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01872-x
更新日期:2000-08-11 00:00:00
abstract::The trk family of tyrosine protein kinase genes serves crucial roles for the development of the nervous system and the survival of neurons. The members of this gene family, trk, trkB and trkC, bind a distinct neurotrophin of the nerve growth factor (NGF) gene family, and trigger the intracellular signals which elicit ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80216-h
更新日期:1993-08-23 00:00:00
abstract::Histone H1(0) is found in tissues with little or no cellular proliferation and has been shown to accumulate during cellular terminal differentiation. Two subtypes of H1(0), H1 a and H1 b, are present in any tissue where the protein has been detected. We report here the first evidence of an age-dependent change in the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81328-5
更新日期:1987-01-05 00:00:00
abstract::Gastrin (G17) and N-carboxymethylgastrin (G17-Gly) have been shown to stimulate the growth of colon cancer cells both in vivo and in vitro. The identity of the receptor mediating these effects is controversial. A recent study demonstrated the presence of a low affinity binding site for G17 and G17-Gly on the DLD-1 hum...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01408-x
更新日期:2004-01-02 00:00:00
abstract::During the course of large scale purification of the D1 dopamine receptor from rat brain, a protein of approximately 87,000 daltons (p87) was observed to copurify with the D1 receptor through four chromatographic steps. To characterize the nature of this protein, bovine and rat cDNA clones were isolated and sequenced....
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80917-6
更新日期:1992-11-09 00:00:00
abstract::Three novel neuropeptides, isolated from ganglia of the African giant snail, Achatina fulica, were named WWamide-1, -2 and -3. These substances were biologically active heptapeptide amides with a Trp residue at both the N- and C-termini. WWamide-1, which displayed an inhibitory activity on a central neuron of the snai...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81458-c
更新日期:1993-05-24 00:00:00
abstract::The tryptophan analog, 5-hydroxytryptophan (5HW), has a significant absorbance between 310-320 nm, which allows it to act as an exclusive fluorescence probe in protein mixtures containing a large number of tryptophan residues. Here for the first time a method is reported for the biosynthetic incorporation of 5HW into ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81346-n
更新日期:1992-10-05 00:00:00
abstract::Acaryochloris marina is a prochlorophyte-like cyanobacterium containing both phycobilins and chlorophyll d as light harvesting pigments. We show that the chlorophyll d light harvesting system, composed of Pcb proteins, functionally associates with the photosystem II (PSII) reaction center (RC) core to form a giant sup...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.01.023
更新日期:2005-02-14 00:00:00
abstract::Nine analogs of fusion peptide of influenza virus hemagglutinin whose membrane perturbation activity has been thoroughly tested [Murata et al. (1992) Biochemistry 31, 1986-1992; Murata et al. (1993) Biophys. J. 64, 724-734] were characterized by molecular modeling techniques with the aim of delineating any specific st...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01505-7
更新日期:1999-11-26 00:00:00
abstract::The isolation of a brain peptide identical to the intestinal peptide PHI (peptide HI) is described. The peptide was isolated from porcine brain extract using a chemical assay method based on its C-terminal isoleucine amide structure. The complete amino acid sequence of the peptide was found to be: His-Ala-Asp-Gly-Val-...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80617-6
更新日期:1983-03-21 00:00:00
abstract::The metabolite-modulated association of a fraction of hexokinase to mitochondria in brain is well documented, however, the involvement of other non-mitochondrial components in the binding of the hexokinase is controversial. Now we present evidence that the hexokinase binds both tubulin and microtubules in brain in vit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03136-2
更新日期:2001-11-30 00:00:00
abstract::Four 12.2-12.6 kDa small heat-shock proteins (sHSPs) of Caenorhabditis elegans are the smallest known members of the sHSP family. They essentially comprise the characteristic C-terminal 'alpha-crystallin domain' of the sHSPs, having a very short N-terminal region, and lacking a C-terminal tail. Recombinant Hsp12.2 and...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00917-x
更新日期:1998-08-21 00:00:00
abstract::H2O2 addition to the oxidized cytochrome c oxidase reconstituted in liposomes brings about a red shift of the Soret band of the enzyme and an increased absorption in the visible region with two distinct peaks at approximately 570 and 605 nm. Throughout pH range 6-8.5, the spectral changes at 570 nm and in the Soret ba...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80258-2
更新日期:1987-07-27 00:00:00
abstract::Atrial natriuretic factor (ANF) and C-type natriuretic peptide (CNP)-activated guanylate cyclases are single-chain transmembrane-spanning proteins, containing both ligand binding and catalytic activities. In both proteins, ligand binding to the extracellular receptor domain activates the cytosolic catalytic domain, ge...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80408-m
更新日期:1993-12-13 00:00:00
abstract::In fission yeast, the only known essential function of Ned8p is the modification of the cullin, Pcu1p, and subsequent Cullin-RING-Ligase (CRL) activation and substrate ubiquitination. We show here that a functional Pcu1p mutant, deleted for its C-terminal autoinhibitory domain, which negates the requirement of neddyla...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.04.011
更新日期:2012-05-21 00:00:00
abstract::[3H]inositol-labelled products are released from adrenal medullary cells during exocytotic secretion. In 'leaky' cells in which small molecules readily enter and leave the cytoplasm, addition of micromolar calcium ions in the presence of ATP stimulates exocytosis and causes the release of inositol polyphosphates. Thes...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80858-9
更新日期:1985-09-09 00:00:00
abstract::Ribosome display is a powerful tool for selecting and evolving protein functions through ligand-binding. Here, this in vitro system was used to perform selection based on the folding properties of proteins, independent of specific ligand-binding. The selection is based on two properties of misfolded proteins: (1) incr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00178-9
更新日期:2003-03-27 00:00:00
abstract::Sequence comparison based on Hydrophobic Cluster Analysis procedures shows that the extracellular approximately 200 amino acids domains of cytokines receptors belonging to the Cytokine/Growth hormone/Prolactin receptor family and to the Interferon one are organized in two homologous subdomains. Further, comparison of ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80437-8
更新日期:1991-04-22 00:00:00
abstract::The crawling locomotion and shape of eukaryotic cells have been associated with the stochastic molecular dynamics of actin and its protein regulators, chiefly Arp2/3 and Rho family GTPases, in making a cytoskeleton meshwork within cell extensions. However, the cell's actin-dependent oscillatory shape and extension dyn...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03207-0
更新日期:2002-01-02 00:00:00
abstract::The inhibitory effect of actin on protein phosphorylation by three distinct protein kinases (CK-II, A-kinase and MAP-kinase) was examined in vitro. It was found that: (i) actin inhibits the activities of alpha-monomeric CK-II (CK-IIalpha) as well as oligomeric CK-II (alpha2beta2) in a dose-dependent manner, but has no...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01266-5
更新日期:1996-12-02 00:00:00
abstract::Apoptotic proteases cleave and inactivate survival signaling molecules such as Akt/PKB, phospholipase C (PLC)-gamma1, and Bcl-2. We have found that treatment of A431 cells with tumor necrosis factor-alpha in the presence of cycloheximide resulted in the cleavage of epidermal growth factor receptor (EGFR) as well as th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02167-6
更新日期:2001-02-23 00:00:00
abstract::The unfolded protein response (UPR)--the endoplasmic reticulum stress response--is found in various pathologies including ischemia-reperfusion injury (IRI). However, its role during IRI is still unclear. Here, by combining two different bioinformatical methods--a method based on ordinary differential equations (Time S...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.05.065
更新日期:2014-08-25 00:00:00
abstract::Oligopeptidase B (OpdB) from Trypanosoma brucei is a candidate therapeutic target in African trypanosomiasis. OpdB is an atypical serine peptidase, since activity is inhibited by thiol-blocking reagents and enhanced by reducing agents. We have identified C256 as the reactive cysteine residue that mediates OpdB inhibit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.03.014
更新日期:2005-04-11 00:00:00