Evidence for two H2O2-binding sites in ferric cytochrome c oxidase. Indication to the O-cycle?

Abstract:

:H2O2 addition to the oxidized cytochrome c oxidase reconstituted in liposomes brings about a red shift of the Soret band of the enzyme and an increased absorption in the visible region with two distinct peaks at approximately 570 and 605 nm. Throughout pH range 6-8.5, the spectral changes at 570 nm and in the Soret band titrate with very similar pH-independent Kd values of 2-3 microM. At the same time, Kd of the peroxide complex measured at 605 nm increases markedly with increased H+ activity reaching the value of 18 +/- 2 microM at pH 6.0. This finding may indicate the presence of two different H2O2-binding sites in the enzyme with different affinity for the ligand at acid pH. The Soret and 570 nm band effects are suggested to report H2O2 coordination to heme iron of alpha 3, whereas the maximum at 605 nm could arise from H2O2 binding to Cu alpha 3 followed by the enzyme transition into the 'pulsed' (or '420/605') conformation. Possible implication of the two H2O2-binding sites for the cytochrome oxidase redox and proton-pumping mechanisms are discussed.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Vygodina T,Konstantinov AA

doi

10.1016/0014-5793(87)80258-2

subject

Has Abstract

pub_date

1987-07-27 00:00:00

pages

387-92

issue

2

eissn

0014-5793

issn

1873-3468

pii

0014-5793(87)80258-2

journal_volume

219

pub_type

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