Abstract:
:In fission yeast, the only known essential function of Ned8p is the modification of the cullin, Pcu1p, and subsequent Cullin-RING-Ligase (CRL) activation and substrate ubiquitination. We show here that a functional Pcu1p mutant, deleted for its C-terminal autoinhibitory domain, which negates the requirement of neddylation for ligase activity, is unable to rescue the loss of neddylation. These findings suggest that the neddylation of non-cullin substrate(s) are required for Schizosaccharomyces pombe viability.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Girdwood D,Robertson M,Gordon Cdoi
10.1016/j.febslet.2012.04.011subject
Has Abstractpub_date
2012-05-21 00:00:00pages
1522-8issue
10eissn
0014-5793issn
1873-3468pii
S0014-5793(12)00289-Xjournal_volume
586pub_type
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