Abstract:
:The interconversions of the inositol-containing lipids (PI, PI-P and PI-P2) and their products (DG, inositol phosphates and PA) in human and rabbit neutrophils stimulated with fMetLeuPhe and PMA have been examined. PMA causes only the phosphorylation of PI to PI-P whereas fMetLeuPhe causes phosphorylation of both PI and PI-P yielding PI-P2 and the hydrolysis of all three lipids. While the predominant reaction is breakdown of PI to PA catalysed by phospholipase D, approx. 2% of PI is converted to polyphosphoinositides and then broken down by the phospholipase C route yielding inositol phosphates and DG. The latter reaction occurs without detectable lag and is a function of receptor occupancy. The amount of inositol trisphosphate thus formed would be sufficient to release Ca2+ from intracellular stores.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Cockcroft S,Barrowman MM,Gomperts BDdoi
10.1016/0014-5793(85)80271-4subject
Has Abstractpub_date
1985-02-25 00:00:00pages
259-63issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(85)80271-4journal_volume
181pub_type
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