Abstract：
:We have studied the endosome-lysosome transfer of internalized epidermal growth factor receptor (EGFR) complexes in a cell-free system from rat liver. Analytical subfractionation of a postmitochondrial supernatant fraction showed that a pulse of internalized [(125)I]EGF was largely associated with a light endosomal fraction devoid of lysosomal markers. After an additional 30 min incubation in vitro in the presence of an ATP-regenerating system, the amount of [(125)I]EGF in this compartment decreased by 39%, with an increase in [(125)I]EGF in lysosomes. No transfer of [(125)I]EGF to the cytosol was detected. To assess the fate of the internalized EGFR protein over the time course of the endo-lysosomal transfer of the ligand, the effect of a saturating dose of native EGF on subsequent lysosomal targeting of the EGFR was evaluated by immunoblotting. A massive translocation of the EGFR to the endosomal compartment was observed in response to ligand injection coincident with its tyrosine phosphorylation and receptor recruitment of the tyrosine-phosphorylated adaptor protein Shc. During cell-free endosome-lysosome fusion, a time-dependent increase in the content of the EGFR and the two 55- and 46-kDa Shc isoforms was observed in lysosomal fractions with a time course superimposable with the lysosomal transfer of the ligand; no transfer of the 66-kDa Shc isoform was detected. The relationship between EGFR tyrosine kinase activity and EGFR sorting in endosomes investigated by immunoblot studies with anti-phosphotyrosine antibodies revealed that endosomal dephosphorylation of EGFR and Shc preceded lysosomal transfer. These results support the view that a lysosomal targeting machinery distinct from the endosomal receptor kinase activity, such as the recruitment of the signaling molecule Shc, may regulate this sorting event in the endosome.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Authier F,Chauvet Gdoi
10.1016/s0014-5793(99)01413-1keywords:
subject
Has Abstractpub_date
1999-11-12 00:00:00pages
25-31issue
1-2eissn
0014-5793issn
1873-3468pii
S0014-5793(99)01413-1journal_volume
461pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract：:Using RT-PCR we distinguished mRNAs for all known G-protein coupled serotonin receptors expressed in various rat and porcine blood vessels. Nearly all vessels expressed 5HT1D beta, 5-HT2A, 5-HT2B, 5-HT4, and 5-HT7 receptor mRNA to different extents. New splice variants of the porcine 5-HT4 receptor were observed. Simi...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/0014-5793(95)00828-w
更新日期：1995-08-21 00:00:00
abstract：:Many proteins located in the intermembrane space (IMS) of mitochondria are characterized by a low molecular mass, contain highly conserved cysteine residues and coordinate metal ions. Studies on one of these proteins, Tim13, revealed that net translocation across the outer membrane is driven by metal-dependent folding...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/j.febslet.2004.11.072
更新日期：2005-01-03 00:00:00
abstract：:Ycf1p function is regulated by casein kinase 2α, Cka1p, via phosphorylation of Ser251. Cka1p-mediated phosphorylation of Ycf1p is attenuated in response to high salt stress. Previous results from our lab suggest a role for Ycf1p in cellular resistance to salt stress. Here, we show that Ycf1p plays an important role in...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/j.febslet.2012.02.010
更新日期：2012-03-23 00:00:00
abstract：:CpG dinucleotides are efficiently methylated in vertebrate genomes except in the CpG islands having a high C+G content. Methylated CpGs are the single most mutated dinucleotide. Sequences surrounding disease causing CpG mutation sites were analyzed from locus-specific mutation databases. Both tetra- and heptanucleotid...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/0014-5793(96)01075-7
更新日期：1996-11-04 00:00:00
abstract：:The precise interactions between the subunits of Gs (alpha s, beta, gamma) and the plasma membrane remain to be established. If alpha s is associated loosely with the inner membrane, is labile during activation, or is always present to some extent in the cytoplasm, then it should fractionate to the supernatant of a hi...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/0014-5793(92)80400-b
更新日期：1992-01-13 00:00:00
abstract：:We have explored the mechanisms involved in the facilitation of glutamate release mediated by the activation of kainate receptors (KARs) in the cortex using isolated nerve terminals (synaptosomes). Kainate (KA) produced an increase on glutamate release at 100 μM. The effect of KA was antagonized by NBQX (with AMPA rec...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/j.febslet.2013.01.071
更新日期：2013-03-18 00:00:00
abstract：:We tested the truncated 7-37 glucagon-like peptide 1 (TGLP-1), a naturally occurring porcine intestinal peptide, and other members of the glucagon family, including pancreatic glucagon (G-29), GLP-1 and GLP-2 for their ability to activate the cAMP generating system in rat gastric glands and HGT-1 human gastric cancer ...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/0014-5793(88)80297-7
更新日期：1988-08-15 00:00:00
abstract：:Under heat shock conditions translation of Xenopus laevis normal mRNAs in a rabbit reticulocyte cell-free system is blocked whereas hsp70 mRNA is translated. mRNA for E. coli beta-galactosidase containing the last four sense codons of Drosophila hsp70 at its 3'-end was constructed. This mRNA is efficiently translated ...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/0014-5793(89)81346-8
更新日期：1989-04-24 00:00:00
abstract：:Hydrogenase gene from Clostridium butyricum was cloned in Escherichia coli HK16 (Hyd-) using pBR322 and PstI. The plasmid, pCBH1, containing hydrogenase gene was 7.3 MDa and pCBH1 had 5 PstI-DNA fragments (3.9, 2.6, 0.7, 0.03-0.04, less than 0.02 MDa, respectively). The hydrogenase activity of HK16 (pCBH1) was about 3...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/0014-5793(83)80689-9
更新日期：1983-07-11 00:00:00
abstract：:The insulin receptor family consists of the homologous tyrosine kinase receptors, insulin receptor (IR), insulin-like growth factor 1 receptor (IGF1R) and insulin receptor-related receptor. The three-dimensional structures of the tyrosine kinase domain of the IR and the first three extracellular domains (L1, Cys-rich ...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/s0014-5793(98)01509-9
更新日期：1998-12-18 00:00:00
abstract：:We studied the molecular mechanism underlying PDT-induced apoptosis-dependent macrophage activation, particularly through NO production. We demonstrate that NO production is initially induced by HSP70 on the apoptotic cell surface, and is further enhanced by macrophage phagocytosis. Additionally, we found that apoptot...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/j.febslet.2012.11.026
更新日期：2013-01-16 00:00:00
abstract：:FITC-labeled LPS from Neisseria meningitidis can be used as a probe to follow the process of LPS incorporation into liposomal membrane and to study its interaction with a bilayer. The incorporation of FITC-LPS into the bilayer was proved by physicochemical methods as well as by liposomal LPS toxicity decrease in actin...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/0014-5793(90)81123-6
更新日期：1990-08-20 00:00:00
abstract：:We have developed a high-throughput sequencing approach that enables us to determine terminal telomere sequences from tens of thousands of individual Schizosaccharomyces pombe telomeres. This method provides unprecedented coverage of telomeric sequence complexity in fission yeast. S. pombe telomeres are composed of mo...
journal_title：FEBS letters
pub_type： 信件
doi：10.1002/1873-3468.12444
更新日期：2016-12-01 00:00:00
abstract：:The protein RPE65 has an important role in retinoid processing and/or retinoid transport in the eye. Retinoids are involved in cell differentiation, embryogenesis and carcinogenesis. Since the kidney is known as an important site for retinoid metabolism, the expression of RPE65 in normal kidney and transformed kidney ...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/s0014-5793(99)00606-7
更新日期：1999-06-11 00:00:00
abstract：:The serine peptidases, thrombocytin and PA-BJ, isolated from the venom of Bothrops atrox and Bothrops jararaca, respectively, induce platelet aggregation and granule secretion without clotting fibrinogen. The specific platelet aggregation activity of each enzyme was about 15 times lower than that of thrombin. This act...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/s0014-5793(00)01803-2
更新日期：2000-07-21 00:00:00
abstract：:An experimental procedure is described for the reproducible production of hybridoma cells using the electrofusion technique. High yields can be obtained when fusion is performed in isotonic inositol solutions containing Ca2+ and Mg2+ in a ratio of 1:5 in the millimolar range. The hybridoma cells are transferred 10 min...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/0014-5793(85)80315-x
更新日期：1985-03-25 00:00:00
abstract：:To assure fidelity of translation, class Ia aminoacyl-tRNA synthetases (aaRSs) edit mis-aminoacylated tRNAs. Mis-attached amino acids and structural water molecules are not included simultaneously in the current crystal structures of the aaRSctRNA complexes, where the 3'-ends (adenine 76; A76) are bound to the editing...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/j.febslet.2009.01.049
更新日期：2009-02-18 00:00:00
abstract：:Nucleoside analogues previously found to be inactive against the human immunodeficiency virus (HIV) may be activated by simple chemical derivatisation. As part of our effort to deliver masked phosphates inside living cells we have discovered that certain phosphate triester derivatives of inactive nucleoside analogues ...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/0014-5793(93)81580-s
更新日期：1993-05-17 00:00:00
abstract：:In addition to the classical functions of the Golgi in membrane transport and glycosylation, the Golgi apparatus of mammalian cells is now recognised to contribute to the regulation of a range of cellular processes, including mitosis, DNA repair, stress responses, autophagy, apoptosis and inflammation. These processes...
journal_title：FEBS letters
pub_type： 杂志文章,评审
doi：10.1002/1873-3468.13567
更新日期：2019-09-01 00:00:00
abstract：:Plants have evolved to cope with fluctuations in water supply by gating their water channels known as aquaporins. During flooding, a rapid drop of cytosolic pH due to anoxia leads to a simultaneous closure of the aquaporins in the plasma membrane. The closing mechanism has been suggested to involve a conserved histidi...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/j.febslet.2013.02.038
更新日期：2013-04-02 00:00:00
abstract：:The relationship between active transport of lactose via the lactose permease and the protonmotive force has been determined in E. coli cells using either the respiratory chain inhibitor cyanide or protonophores to decrease the protonmotive force progressively. In contradiction with the prediction of the delocalized c...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/0014-5793(86)81136-x
更新日期：1986-12-15 00:00:00
abstract：:By sequence analysis we show that the U104 domain found in the UNC104 subfamily of kinesins is a forkhead homology-associated domain (FHA). A combination of limited proteolysis, mass spectroscopy, and physicochemical analysis define this domain as a genuine autonomously folding domain. Our data show that the FHA domai...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/s0014-5793(00)02310-3
更新日期：2000-12-15 00:00:00
abstract：:After initial purification of ovine placental lactogen (oPL) using the procedures described previously [(1976) Endocrinology 98, 65-75], the oPL preparation was further purified by high-performance liquid chromatography (HPLC) using an anionic exchange column (Bio-Sil TSK DEAE-2-SW). Two forms of oPL with different re...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/0014-5793(86)80491-4
更新日期：1986-04-21 00:00:00
abstract：:The acronym CK2 denotes a highly pleiotropic Ser/Thr protein kinase whose over-expression correlates with neoplastic growth. A vexed question about the enigmatic regulation of CK2 concerns the actual existence in living cells of the catalytic (alpha and/or alpha') and regulatory beta-subunits of CK2 not assembled into...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/j.febslet.2006.06.031
更新日期：2006-07-10 00:00:00
abstract：:The temperature dependence of the rate constants of the univalent redox steps YzoxSi----YzSi + 1 (i = 0,1,2) and YzoxS3----(YzS4)----YzSo + O2 in the water oxidase was investigated by measuring time resolved absorption changes at 355 nm induced by a laser flash train in dark adapted PS II membrane fragments from spina...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/0014-5793(92)80092-u
更新日期：1992-03-24 00:00:00
abstract：:Calmodulin is shown to interact with human spectrin dimer. The binding was highly calcium-dependent and observed in two different kinds of experiments. Firstly, affinity chromatography of calmodulin on a Sepharose 4B column with immobilized spectrin, and secondly, partition in aqueous two-phase polymer systems. In the...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/0014-5793(84)80884-4
更新日期：1984-06-25 00:00:00
abstract：:Vasopressin elicited a dose-dependent inhibition of glucagon-induced cAMP accumulation in isolated hepatocytes. This response was not diminished by incubation of cells with the calmodulin antagonists trifluoperazine or chlorpromazine and was only slightly reduced in Ca2+-depleted hepatocytes. Half-maximal inhibition o...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/0014-5793(83)80835-7
更新日期：1983-11-14 00:00:00
abstract：:Lung surfactant secretion is a highly regulated process. Our previous studies have shown that VAMP-2 is essential for surfactant secretion. In the present study we investigated the role of miR-206 in surfactant secretion through VAMP-2. VAMP-2 was confirmed to be a target of miR-206 by 3'-untranslational region (3'-UT...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/j.febslet.2014.11.043
更新日期：2015-01-02 00:00:00
abstract：:We have identified in rat vascular smooth muscle cells (SMCs) the simultaneous expression of two TGF-beta type I receptor (ALK-5) cDNAs, occurring as a consequence of alternate usage of AG splice acceptor motifs separated by 12 nucleotides located at an intron-exon junction. When translated the resultant full length p...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1016/s0014-5793(00)01132-7
更新日期：2000-02-04 00:00:00
abstract：:Chemotherapy is a major anticancer therapeutic modality, however, multidrug resistance (MDR) is frequently observed and hinders treatment efficacy. Here, we investigated the role and potential mechanism of the long noncoding RNA (lncRNA) FENDRR in adriamycin resistance of chronic myeloid leukaemia (CML) cells. FENDRR ...
journal_title：FEBS letters
pub_type： 杂志文章
doi：10.1002/1873-3468.13480
更新日期：2019-08-01 00:00:00
