Abstract:
:Using RT-PCR we distinguished mRNAs for all known G-protein coupled serotonin receptors expressed in various rat and porcine blood vessels. Nearly all vessels expressed 5HT1D beta, 5-HT2A, 5-HT2B, 5-HT4, and 5-HT7 receptor mRNA to different extents. New splice variants of the porcine 5-HT4 receptor were observed. Similar PCR assays were performed with endothelial and smooth muscle cells from human pulmonary artery, aorta, and with endothelial cells from human coronary artery and umbilical vein. All endothelial cells expressed 5-HT1D beta, 5-HT2B, and 5-HT4 receptor mRNA, whereas in smooth muscle cells 5-HT1D beta, 5-HT2A, 5-HT7, and in some experiments 5-HT2B receptor mRNA were found. A model for the regulation of vascular tone by different 5-HT receptors is proposed.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Ullmer C,Schmuck K,Kalkman HO,Lübbert Hdoi
10.1016/0014-5793(95)00828-wsubject
Has Abstractpub_date
1995-08-21 00:00:00pages
215-21issue
3eissn
0014-5793issn
1873-3468pii
0014-5793(95)00828-Wjournal_volume
370pub_type
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