TeloPCR-seq: a high-throughput sequencing approach for telomeres.

abstract：:The in vitro methylation of the +6, +24, and -215 located 5'-CCGG-3' sequences in the late E2A promoter of adenovirus type 2 (Ad2) DNA abrogates promoter function. 5-Methyldeoxycytidine (5-mC) at positions +6 and +24 in both or either of the two DNA complements in the late E2A promoter abolishes the formation of a hig...

journal_title：FEBS letters

authors： Hermann R,Doerfler W

更新日期：1991-04-09 00:00:00

abstract：:Two cDNA clones encoding 14-3-3 homologous proteins were isolated from Vicia faba. Deduced amino acid sequences share different degrees of homology with other plant 14-3-3 proteins. Both clones, under the control of the CaMV 35S promoter, were transformed into tobacco plants. Immunoblotting showed three different form...

journal_title：FEBS letters

authors： Saalbach G,Schwerdel M,Natura G,Buschmann P,Christov V,Dahse I

更新日期：1997-08-18 00:00:00

abstract：:Inducible nitric oxide (NO) synthase (iNOS) appears to be a marker of tumor progression in colon carcinogenesis. Here we investigated effects of NO on selected chemokines that differentially regulate angiogenesis, namely pro-angiogenic interleukin (IL)-8 as well as tumor-suppressive interferon-inducible protein-10 (IP...

journal_title：FEBS letters

authors： Hellmuth M,Paulukat J,Ninic R,Pfeilschifter J,Mühl H

更新日期：2004-04-09 00:00:00

abstract：:The metabolism of the double bonds at the delta 3 position in fatty acids was studied in rat liver. Infusion of delta 3-trans-dodecenoic acid into isolated perfused liver and subcellular fractionation studies showed the presence of both peroxisomal and mitochondrial delta 3,delta 2-enoyl-CoA isomerase activity (EC 5.3...

journal_title：FEBS letters

authors： Kärki T,Hakkola E,Hassinen IE,Hiltunen JK

更新日期：1987-05-11 00:00:00

abstract：:Most geckos are nocturnal forms and possess rod retinas, but some diurnal genera have pure-cone retinas. We isolated cDNAs encoding the diurnal gecko opsins, dg1 and dg2, similar to nocturnal gecko P521 and P467, respectively. Despite the large morphological differences between the diurnal and nocturnal gecko photorec...

journal_title：FEBS letters

authors： Taniguchi Y,Hisatomi O,Yoshida M,Tokunaga F

更新日期：1999-02-19 00:00:00

abstract：:Antiparaoxon immune sera were employed in a new immunoassay based on competition between acetylcholinesterase and antibodies for the binding of paraoxon. Unlike radioimmunoassay, the new assay described herein can be extended to predict the feasibility of antibodies to confer in vivo protection of acetylcholinesterase...

journal_title：FEBS letters

authors： Heldman E,Balan A,Horowitz O,Ben-Zion S,Torten M

更新日期：1985-01-28 00:00:00

abstract：:We report a nonenzymatic reaction which results in the radiolabeling of proteins by 32P-containing nucleoside triphosphates. The labeling reaction does not require any cofactors, but is greatly enhanced by the presence of alcohols. Even under optimal conditions, less than 1% of the protein molecules undergo modificati...

journal_title：FEBS letters

authors： Schmidt MC,Hanna MM

更新日期：1986-01-06 00:00:00

abstract：:The cholesterol content of rat liver plasma membranes was manipulated using either cholesterol-free or cholesterol-enriched liposomes. Removal of cholesterol from the membranes led to a marked increase in 5'-nucleotidase activity. However, increase in cholesterol content failed to exert any significant effect on 5'-nu...

journal_title：FEBS letters

authors： Whetton AD,Houslay MD

更新日期：1983-06-27 00:00:00

abstract：:In fibrous connective tissues, fibroblasts are organized into syncytia, cellular networks that enable matrix remodeling and that are interconnected by intercellular adherens junctions (AJs). The AJs of fibroblasts are mediated by N-cadherin, a broadly expressed classical cadherin that is critically involved in develop...

journal_title：FEBS letters

authors： El Sayegh TY,Kapus A,McCulloch CA

更新日期：2007-01-23 00:00:00

abstract：:The interaction of bovine pancreatic trypsin inhibitor (BPTI) and bovine tryptase, which are co-localized in the same granules of bovine mast cells, has been analyzed at 30 degrees C in 0.1 M Tris-HCl, pH 8.0. The analysis has unravelled that the functional unit of bovine tryptase is formed of (at least) four binding ...

journal_title：FEBS letters

authors： Fiorucci L,Erba F,Coletta M,Ascoli F

更新日期：1995-04-17 00:00:00

abstract：:We studied the effects of fibroblast growth factor (FGF-10) on alveolar epithelial cell (AEC) Na,K-ATPase regulation. Within 30 min FGF-10 increased Na,K-ATPase activity and alpha1 protein abundance by 2.5-fold at the AEC plasma membrane. Pretreatment of AEC with the mitogen-activated protein kinase (MAPK) inhibitor U...

journal_title：FEBS letters

authors： Upadhyay D,Lecuona E,Comellas A,Kamp DW,Sznajder JI

更新日期：2003-06-19 00:00:00

abstract：:Snake venoms are rich sources of serine proteinase inhibitors that are members of the Kunitz/BPTI (bovine pancreatic trypsin inhibitor) family. However, only a few of their gene sequences have been determined from snakes. We therefore cloned the cDNAs for the trypsin and chymotrypsin inhibitors from a Vipera ammodytes...

journal_title：FEBS letters

authors： Zupunski V,Kordis D,Gubensek F

更新日期：2003-07-17 00:00:00

abstract：:Vesicular monoamine transporters (VMAT) catalyze transport of serotonin, dopamine, epinephrine and norepinephrine into subcellular storage organelles in a variety of cells. Accumulation of the neurotransmitter depends on the proton electrochemical gradient across the organelle membrane and involves VMAT-mediated excha...

journal_title：FEBS letters

authors： Shirvan A,Laskar O,Steiner-Mordoch S,Schuldiner S

更新日期：1994-12-12 00:00:00

abstract：:Dystrophin, a component of the muscle membrane cytoskeleton, is the protein altered in Duchenne Muscular Dystrophy (DMD) and Becker Muscular Dystrophy (BMD). Dystrophin shares significant homology with other cytoskeletal proteins, such as alpha-actinin and spectrin. On the basis of its sequence similarity with alpha-a...

journal_title：FEBS letters

authors： Chan Y,Kunkel LM

更新日期：1997-06-30 00:00:00

abstract：:The effect of angiotensin II (Ang II) on the early growth response gene-1 (Egr-1) mRNA, on the Egr-1 protein and on the phosphoinositide PI turnover signalling system was investigated in the presence and absence of EXP3174, a potent non-peptide Ang II receptor antagonist. Ang II induced an accumulation of 3.4 kb Egr-1...

journal_title：FEBS letters

authors： Sachinidis A,Weisser P,Ko Y,Schulte K,Meyer zu Brickwedde K,Neyses L,Vetter H

更新日期：1992-11-23 00:00:00

abstract：:The effects of progesterone, dehydroepiandrosterone sulfate and 17beta-estradiol on the synthesis and degradation of hyaluronate were investigated using human uterine cervix fibroblasts. The cells were incubated with [3H]glucosamine in the presence of the hormones and then [3H]hyaluronate was isolated from the medium....

journal_title：FEBS letters

authors： Tanaka K,Nakamura T,Takagaki K,Funahashi M,Saito Y,Endo M

更新日期：1997-02-03 00:00:00

abstract：:Kinetic analyses of cpTat-mediated protein transport across the thylakoid membrane were conducted, revealing three important characteristics of this translocation pathway. First, transport via the cpTAT system displays a non-Michaelis-Menten, sigmoidal rate-substrate relationship with an apparent Hill coefficient of 1...

journal_title：FEBS letters

authors： Alder NN,Theg SM

更新日期：2003-04-10 00:00:00

abstract：:Gamma-aminobutyric acid type A (GABAA) receptor beta1 (gabrb1), a subunit of GABAA receptors involved in inhibitory effects on neurotransmission, was found to associate with the formation of protease-resistant prion protein in prion-infected neuroblastoma cells. Silencing of gabrb1 gene expression significantly decrea...

journal_title：FEBS letters

authors： Kimura T,Ishikawa K,Sakasegawa Y,Teruya K,Sata T,Schätzl H,Doh-ura K

更新日期：2010-03-19 00:00:00

abstract：:In Clostridium thermoaceticum, the synthesis of acetyl-CoA from methyl tetrahydrofolate occurs via a series of enzymatic reactions involving methyl transferase, corrinoid/Fe-S protein (corrinoid), carbon monoxide dehydrogenase (CODH) and ferredoxin. We have investigated the possibility of one or more of these proteins...

journal_title：FEBS letters

authors： Shanmugasundaram T,Sundaresh CS,Kumar GK

更新日期：1993-07-12 00:00:00

abstract：:Streptomyces lividans 1326-9, a bldA+ strain, and its bldA39 mutant derivative J1725 were transformed with a cosmid containing the pur cluster, which determines the puromycin biosynthetic pathway from Streptomyces alboniger. bldA+ transformants produced puromycin in typical amounts, whereas bldA39 transformants did so...

journal_title：FEBS letters

authors： Tercero JA,Espinosa JC,Jiménez A

更新日期：1998-01-16 00:00:00

abstract：:Periplasmic adaptor proteins are essential components of bacterial tripartite multidrug efflux pumps. Here we report the 2.35 Å resolution crystal structure of the BesA adaptor from the spirochete Borrelia burgdorferi solved using selenomethionine derivatized protein. BesA shows the archetypal linear, flexible, multi-...

journal_title：FEBS letters

authors： Greene NP,Hinchliffe P,Crow A,Ababou A,Hughes C,Koronakis V

更新日期：2013-09-17 00:00:00

abstract：:A novel calcium-dependent serine proteinase (CASP) secreted from malignant hamster embryo fibroblast Ni 12C2 degrades extracellular matrix proteins. A complementary DNA encoding CASP has been isolated with the use of oligonucleotide probes synthesized based on partial amino acid sequences of CASP. The complete amino a...

journal_title：FEBS letters

authors： Kinoshita H,Sakiyama H,Tokunaga K,Imajoh-Ohmi S,Hamada Y,Isono K,Sakiyama S

更新日期：1989-07-03 00:00:00

abstract：:Insight into the regulation of the actions of the protein-tyrosine kinases will be greatly facilitated by the full characterization of the family of protein-tyrosine phosphatases. A search for novel phosphatases resulted in the isolation of a cDNA, termed HLPR, encoding a member of the family of human receptor-like pr...

journal_title：FEBS letters

authors： Jirik FR,Janzen NM,Melhado IG,Harder KW

更新日期：1990-10-29 00:00:00

abstract：:Higher basal 2-deoxy-D-glucose uptake in rheumatoid synovial cells than in non-rheumatoid synovial cells, was found to be associated with an increased interleukin-1 beta (IL-1 beta) secretion (respectively 850 +/- 238 vs. 8.3 +/- 2.4 pg/24 h/10(5) cells, mean +/- S.E.M.). When exogenous human recombinant IL-1 beta was...

journal_title：FEBS letters

authors： Hernvann A,Aussel C,Cynober L,Moatti N,Ekindjian OG

更新日期：1992-05-25 00:00:00

abstract：:Incubation of rat skeletal muscle mitochondria with the nitric oxide generator, S-nitrosoglutathione (GSNO) reversibly inhibited oxygen utilisation with all substrates tested. The visible absorption spectra of the inhibited mitochondria showed that cytochromes c+c1, b and a+a3 were reduced, indicating a block at the d...

journal_title：FEBS letters

authors： Cleeter MW,Cooper JM,Darley-Usmar VM,Moncada S,Schapira AH

更新日期：1994-05-23 00:00:00

abstract：:In a wild-type strain of Saccharomyces cerevisiae, cadmium induces the activities of both gamma-glutamyl transferase (gamma-GT) and glutathione transferase 2 (Gtt2). However, Gtt2 activity did not increase under gamma-GT or Ycf1 deficiencies, suggesting that the accumulation of glutathione-cadmium in the cytosol inhib...

journal_title：FEBS letters

authors： Adamis PD,Mannarino SC,Eleutherio EC

更新日期：2009-05-06 00:00:00

abstract：:Temporary inhibition of the cysteine proteinases papain and cathepsin L was observed with several hairpin loop mutants of recombinant chicken cystatin at enzyme concentrations above nanomolar. Kinetic modelling of inhibition data, gel electrophoresis and amino acid sequencing revealed that reappearance of papain activ...

journal_title：FEBS letters

authors： Machleidt W,Nägler DK,Assfalg-Machleidt I,Stubbs MT,Fritz H,Auerswald EA

更新日期：1995-03-20 00:00:00

abstract：:Smad ubiquitination regulatory factor 2 (Smurf2), a ubiquitin ligase for Smads, plays critical roles in the regulation of transforming growth factor-beta (TGF-beta)-Smad signaling via ubiquitin-dependent degradation of Smad2 and Smad7. We found that TGF-beta stimulates Smurf2 expression. TGF-beta activated the Smurf2 ...

journal_title：FEBS letters

authors： Ohashi N,Yamamoto T,Uchida C,Togawa A,Fukasawa H,Fujigaki Y,Suzuki S,Kitagawa K,Hattori T,Oda T,Hayashi H,Hishida A,Kitagawa M

更新日期：2005-05-09 00:00:00

abstract：:Seryl-tRNA synthetases (SerRSs) fall into two distinct evolutionary groups of enzymes, bacterial and methanogenic. These two types of SerRSs display only minimal sequence similarity, primarily within the class II conserved motifs, and possess distinct modes of tRNA(Ser) recognition. In order to determine whether the t...

journal_title：FEBS letters

authors： Ahel D,Slade D,Mocibob M,Söll D,Weygand-Durasevic I

更新日期：2005-08-15 00:00:00

abstract：:Deuterated arachidonic acid (AA, [2H8]20:4 n-6) 1-25 microM, is converted to other fatty acids, as evaluated by gas chromatography-mass spectrometry, in THP-1 cells. The major products, in the 1 to 10 microM range, are 22:4 (elongated) and 20:3 (reduced in 5). At 25 microM, 24:4, 24:5 and 22:5 accumulate, with [2H8]/[...

journal_title：FEBS letters

authors： Caruso D,Risé P,Galella G,Regazzoni C,Toia A,Galli G,Galli C

更新日期：1994-05-02 00:00:00