FITC-labeled lipopolysaccharide: use as a probe for liposomal membrane incorporation studies.

abstract：:The intestinal microbiota is essential for nutrient acquisition, immune development, and exclusion of invading pathogens. The upper respiratory tract (URT) microbiota is less well studied and does not appear to abide by many of the paradigms of the gastrointestinal tract. Decades of carriage studies in children have d...

journal_title：FEBS letters

authors： Schenck LP,Surette MG,Bowdish DM

更新日期：2016-11-01 00:00:00

abstract：:Chimeric antibodies composed of mouse-derived variable regions and human-derived constant regions have been developed for clinical use. However, construction of chimeric mouse/human genes in expression vectors is time-consuming work. In this study, we developed convenient vectors for construction of chimeric mouse/hum...

journal_title：FEBS letters

authors： Kameyama K,Imai K,Itoh T,Taniguchi M,Miura K,Kurosawa Y

更新日期：1989-02-27 00:00:00

abstract：:We have examined the formation of DNA triple helices between the oligonucleotides T8XT8 (X = A,C,G,T) and DNA fragments containing the target sequences A8XA8.T8YT8 (X = T,C,G; Y = A,G,C), by DNase I footprinting. We find that A8GA8.T8CT8 yields a footprint with T8CT8 and shows a weaker interaction with T17 and T8GT8. ...

journal_title：FEBS letters

authors： Chandler SP,Fox KR

更新日期：1993-10-11 00:00:00

abstract：:Tropine (tropan-3 alpha-ol) is an intermediate in the formation of hyoscyamine. An acyltransferase activity that can acetylate tropine using acetylcoenzyme A as cosubstrate has been found in transformed root cultures of Datura stramonium. A further acyltransferase activity that acetylates pseudotropine (tropan-3 beta-...

journal_title：FEBS letters

authors： Robins RJ,Bachmann P,Robinson T,Rhodes MJ,Yamada Y

更新日期：1991-11-04 00:00:00

abstract：:A new GTP-binding protein serving as the specific substrate of islet-activating protein (IAP), pertussis toxin, was purified from porcine brain membranes as an alpha beta gamma-heterotrimeric structure. The alpha-subunit of the purified protein (alpha 40 beta gamma) had a molecular mass of 40 kDa and differed from tha...

journal_title：FEBS letters

authors： Katada T,Oinuma M,Kusakabe K,Ui M

更新日期：1987-03-23 00:00:00

abstract：:Human complement component C8gamma is an unusual complement factor since it shows no homology to other complement proteins but is a member of the lipocalin superfamily. So far, it has been found exclusively in plasma, covalently linked to C8alpha by disulfide bridging. We have used dot blot and Northern blot analyses ...

journal_title：FEBS letters

authors： Trojer P,Wojnar P,Merschak P,Redl B

更新日期：1999-03-12 00:00:00

abstract：:Single-pass sequences were obtained from the 5'-ends of a total of 1794 rose petal cDNA clones. Cluster analysis identified 242 groups of sequences and 635 singletons indicating that the database represents a total of 877 genes. Putative functions could be assigned to 1151 of the transcripts. Expression analysis indic...

journal_title：FEBS letters

authors： Channelière S,Rivière S,Scalliet G,Szecsi J,Jullien F,Dolle C,Vergne P,Dumas C,Bendahmane M,Hugueney P,Cock JM

更新日期：2002-03-27 00:00:00

abstract：:To explore the structural basis required for the holoenzyme formation of cAMP-dependent protein kinase, we have prepared rabbit anti-peptide antibodies that can block the holoenzyme formation without affecting the catalytic activity of the enzyme. The antibodies were raised against a specific site in the catalytic (C)...

journal_title：FEBS letters

authors： Sahara S,Sato K,Kaise H,Mori K,Sato A,Aoto M,Tokmakov AA,Fukami Y

更新日期：1996-04-15 00:00:00

abstract：:Hterologous complementation of yeast mutants has enabled the isolation of genes encoding several families of amino acid transporters. Among them, NTR1 codes for a membrane protein with weak histidine transport activity. However, at the sequence level, NTR1 is related to rather non-specific oligopeptide transporters fr...

journal_title：FEBS letters

authors： Rentsch D,Laloi M,Rouhara I,Schmelzer E,Delrot S,Frommer WB

更新日期：1995-08-21 00:00:00

abstract：:The isolation of a brain peptide identical to the intestinal peptide PHI (peptide HI) is described. The peptide was isolated from porcine brain extract using a chemical assay method based on its C-terminal isoleucine amide structure. The complete amino acid sequence of the peptide was found to be: His-Ala-Asp-Gly-Val-...

journal_title：FEBS letters

authors： Tatemoto K,Carlquist M,McDonald TJ,Mutt V

更新日期：1983-03-21 00:00:00

abstract：:The reaction of alpha-chymotrypsin with AcTyr-OEt and with AcTrp-OEt at pH 7.0 and 7.8 was studied over a wide range of substrate concentrations. The reaction with AcTyr-OEt at pH 7.8 was shown to be non-hyperbolic using a variety of criteria whereas those at pH 7.0 with the same substrate and at both pH values with A...

journal_title：FEBS letters

authors： Garrell J,Cuchillo CM

更新日期：1985-10-14 00:00:00

abstract：:We have shown by electrophoretic mobility shift assays that the nucleotide sequence CE-LPH1, centred at position -49 with respect to the transcription start site of the rat gene encoding intestinal lactase-phlorizin hydrolase, interacts in vitro with nuclear proteins present in the jejunum of suckling animals. Protein...

journal_title：FEBS letters

authors： Boukamel R,Freund JN

更新日期：1994-10-10 00:00:00

abstract：:During mRNA decoding at the ribosome, deviations from stringent codon identity, or "mistranslation," are generally deleterious and infrequent. Observations of organisms that decode some codons ambiguously, and the discovery of a compensatory increase in mistranslation frequency to combat environmental stress have chan...

journal_title：FEBS letters

authors： Moghal A,Mohler K,Ibba M

更新日期：2014-11-28 00:00:00

abstract：:The α-subunit of the cardiac voltage-gated sodium channel (NaV1.5) plays a central role in cardiomyocyte excitability. We have recently reported that NaV1.5 is post-translationally modified by arginine methylation. Here, we aimed to identify the enzymes that methylate NaV1.5, and to describe the role of arginine methy...

journal_title：FEBS letters

authors： Beltran-Alvarez P,Espejo A,Schmauder R,Beltran C,Mrowka R,Linke T,Batlle M,Pérez-Villa F,Pérez GJ,Scornik FS,Benndorf K,Pagans S,Zimmer T,Brugada R

更新日期：2013-10-01 00:00:00

abstract：:Based on the amino acid alignment, Caenorhabditis elegans F32D1.1 was identified to be a homologue of the mammalian fidgetin. We produced and purified the F32D1.1 protein by using a baculovirus-expression system. F32D1.1 has an ATPase activity, which is sensitive to N-ethylmaleimide. Km and Vmax for the ATPase activit...

journal_title：FEBS letters

authors： Yakushiji Y,Yamanaka K,Ogura T

更新日期：2004-12-03 00:00:00

abstract：:The R2 protein component of mouse ribonucleotide reductase has been obtained from overproducing Escherichia coli bacteria. It has been crystallized using NaCl as precipitant. The crystals are orthorhombic, space group C222(1), with cell dimensions a = 76.9 A, b = 108.9 A, c = 92.7 A and diffract to at least 2.5 A. The...

journal_title：FEBS letters

authors： Nielsen BB,Kauppi B,Thelander M,Thelander L,Larsen IK,Eklund H

更新日期：1995-10-16 00:00:00

abstract：:Immunometabolism focuses on the mechanisms regulating the impact of metabolism on lymphocyte activity and autoimmunity outbreak. The adipose tissue is long known to release adipokines, either pro- or anti-inflammatory factors bridging nutrition and immune function. More recently, adipocytes were discovered to also rel...

journal_title：FEBS letters

authors： de Candia P,De Rosa V,Gigantino V,Botti G,Ceriello A,Matarese G

更新日期：2017-10-01 00:00:00

abstract：:Limb girdle muscular dystrophy type 2A is linked to a skeletal muscle-specific calpain isoform known as p94. Isolation of the intact 94-kDa enzyme has been difficult to achieve due to its rapid autolysis, and uncertainty has arisen over its Ca2+-dependence for activity. We have expressed a C-terminally truncated form ...

journal_title：FEBS letters

authors： Rey MA,Davies PL

更新日期：2002-12-18 00:00:00

abstract：:The pure lipoxygenase from reticulocytes converts 5,15-di-HETE at 2 degrees C to product(s) showing a characteristic UV spectrum with maxima of strong absorbance at 300 and 316 nm and shoulders at 285 and 350 nm. Their formation was completely prevented by the lipoxygenase inhibitors, ETYA and NDGA, by heating of the ...

journal_title：FEBS letters

authors： Kühn H,Wiesner R,Stender H

更新日期：1984-11-19 00:00:00

abstract：:The -proteobacterium Wolinella succinogenes grows anaerobically by respiratory nitrite ammonification but not by denitrification. Nevertheless, it is capable of N(2)O reduction to N(2). Recently, the genome sequence of W. succinogenes revealed a nos gene cluster with intriguing features encoding a new type of N(2)O re...

journal_title：FEBS letters

authors： Simon J,Einsle O,Kroneck PM,Zumft WG

更新日期：2004-07-02 00:00:00

abstract：:A complete cDNA clone encoding human cholesterol 7 alpha-hydroxylase has been isolated using a rat P-450ch7 alpha cDNA insert [(1989) FEBS Lett. 257, 97-100] as a probe and totally sequenced. The cDNA contained 1512-base pair open reading frame encoding 504 amino acid residues (Mr 57,630), 39-base pair 5'-untranslated...

journal_title：FEBS letters

authors： Noshiro M,Okuda K

更新日期：1990-07-30 00:00:00

abstract：:We have analyzed the glycosaminoglycans exposed on the surface of endothelial cells cultured in vitro. The cells were labelled with [35S]sodium sulfate. Chondroitin sulfate, heparan sulfate and dermatan sulfate are the main sulfated glycosaminoglycans present on the cell surface. They are synthesized by endothelial ce...

journal_title：FEBS letters

authors： Cavari S,Vannucchi S

更新日期：1993-05-24 00:00:00

abstract：:The MET receptor tyrosine kinase is deregulated primarily via overexpression or point mutations in various human cancers and different strategies for MET inhibition are currently evaluated in clinical trials. We observed by Western blot analysis and by Flow cytometry that MET inhibition by different MET small molecule...

journal_title：FEBS letters

authors： Leiser D,Pochon B,Blank-Liss W,Francica P,Glück AA,Aebersold DM,Zimmer Y,Medová M

更新日期：2014-03-03 00:00:00

abstract：:The cytochrome bc(1) complex catalyzes electron transfer from ubiquinol to cytochrome c by a protonmotive Q cycle mechanism in which electron transfer is linked to proton translocation across the inner mitochondrial membrane. In the Q cycle mechanism proton translocation is the net result of topographically segregated...

journal_title：FEBS letters

authors： Hunte C,Palsdottir H,Trumpower BL

更新日期：2003-06-12 00:00:00

abstract：:As a conserved non-coding RNA gene, transcripts of MALAT-1 localize predominately in the nucleus. However in G2/M cell cycle phase, MALAT-1 transcripts were surprisingly found to translocate from the nucleus into the cytoplasm. Investigation also found that in this process MALAT-1 interacts with an abundant nuclear fa...

journal_title：FEBS letters

authors： Yang F,Yi F,Han X,Du Q,Liang Z

更新日期：2013-10-01 00:00:00

abstract：:Glycosylated cellulases from Cellulomonas fimi were compared with their non-glycosylated counterparts synthesized in Escherichia coli from recombinant DNA. Glycosylation of the enzymes does not significantly affect their kinetic properties, or their stabilities towards heat and pH. However, the glycosylated enzymes ar...

journal_title：FEBS letters

authors： Langsford ML,Gilkes NR,Singh B,Moser B,Miller RC Jr,Warren RA,Kilburn DG

更新日期：1987-12-10 00:00:00

abstract：:Interaction of lectins with cell surface determinants may alter membrane properties. Using trypsinized rabbit erythrocytes as model we tested the capacity of an endogenous lectin in this respect. Galectin-1 is a member of an adhesion/growth-regulatory family known to interact for example with ganglioside GM(1) and als...

journal_title：FEBS letters

authors： Gupta RK,Pande AH,Gulla KC,Gabius HJ,Hajela K

更新日期：2006-03-06 00:00:00

abstract：:The functionality of the disintegrin-like/cysteine-rich domains of snake venom metalloproteinases (SVMPs) has been shown to reside in the cysteine-rich region, which can interact with VWA-containing proteins. Recently, the hyper-variable region (HVR) of the cysteine-rich domain was suggested to constitute a potential ...

journal_title：FEBS letters

authors： Menezes MC,Paes Leme AF,Melo RL,Silva CA,Della Casa M,Bruni FM,Lima C,Lopes-Ferreira M,Camargo AC,Fox JW,Serrano SM

更新日期：2008-11-26 00:00:00

abstract：:Pasteurella multocida toxin is a potent mitogen for cultured Swiss 3T3 cells where it causes an accumulation of inositol phosphates and activation of protein kinase C. The gene sequence described here coded for a 146 kDa protein. The ORF was preceded by a ribosome binding site and followed by a stem loop. There was no...

journal_title：FEBS letters

authors： Lax AJ,Chanter N,Pullinger GD,Higgins T,Staddon JM,Rozengurt E

更新日期：1990-12-17 00:00:00

abstract：:VinJ is an amidohydrolase belonging to the serine peptidase family that catalyzes the hydrolysis of the terminal aminoacyl moiety of a polyketide intermediate during the biosynthesis of vicenistatin. Herein, we report the crystal structure of VinJ. VinJ possesses a unique hydrophobic tunnel for the recognition of the ...

journal_title：FEBS letters

authors： Shinohara Y,Miyanaga A,Kudo F,Eguchi T

更新日期：2014-03-18 00:00:00