Discrimination between the activity of protein kinase CK2 holoenzyme and its catalytic subunits.


:The acronym CK2 denotes a highly pleiotropic Ser/Thr protein kinase whose over-expression correlates with neoplastic growth. A vexed question about the enigmatic regulation of CK2 concerns the actual existence in living cells of the catalytic (alpha and/or alpha') and regulatory beta-subunits of CK2 not assembled into the regular heterotetrameric holoenzyme. Here we take advantage of novel reagents, namely a peptide substrate and an inhibitor which discriminate between the holoenzyme and the catalytic subunits, to show that CK2 activity in CHO cells is entirely accounted for by the holoenzyme. Transfection with individual subunits moreover does not give rise to holoenzyme formation unless the catalytic and regulatory subunits are co-transfected together, arguing against the existence of free subunits in CHO cells.




FEBS letters


Salvi M,Sarno S,Marin O,Meggio F,Itarte E,Pinna LA




Has Abstract


2006-07-10 00:00:00














  • Transcriptional and translational control over sodium-glucose-linked transporter (SGLT1) gene expression in adult rat small intestine.

    abstract::We have measured SGLT1 mRNA content and SGLT1-mediated glucose transport at different positions along the small intestine of control and streptozotocin diabetic rats and shown both parameters to be similar but higher in jejunal compared with ileal tissue. No such correlation was seen when comparing measurements of SGL...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Dong R,Srai SK,Debnam E,Smith M

    更新日期:1997-04-07 00:00:00

  • 2,5-Di(tert-butyl)-1,4-benzohydroquinone--a novel inhibitor of liver microsomal Ca2+ sequestration.

    abstract::Treatment of rat liver microsomes with 2,5-di(tert-butyl)-1,4-benzohydroquinone caused a dose-related inhibition (Ki congruent to 1 microM) of ATP-dependent Ca2+ sequestration. This was paralleled by a similar impairment of the microsomal Ca2+-stimulated ATPase activity. In contrast, the hydroquinose failed to induce ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Moore GA,McConkey DJ,Kass GE,O'Brien PJ,Orrenius S

    更新日期:1987-11-30 00:00:00

  • Anion channels and transporters in plant cell membranes.

    abstract::Anion channels/transporters appear as key players in signaling pathways leading to the adaptation of plant cells to abiotic and biotic environmental stresses, in the control of metabolism and in the maintenance of electrochemical gradients. Focusing on the most recent advances, this review aims at providing a descript...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: de Angeli A,Thomine S,Frachisse JM,Ephritikhine G,Gambale F,Barbier-Brygoo H

    更新日期:2007-05-25 00:00:00

  • Polyadenylation of histone mRNA in Xenopus oocytes and embryos.

    abstract::Oogenesis of amphibians is an atypical situation in which histone mRNA is polyadenylated. The poly(A) tract on H4 mRNA has been examined by S1 nuclease analysis. Throughout oogenesis the poly(A) tract is very short, and nonexistent on some mRNA molecules. The poly(A) tract is completely removed during maturation of th...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ballantine JE,Woodland HR

    更新日期:1985-01-28 00:00:00

  • Different epitopes are required for gp130 activation by interleukin-6, oncostatin M and leukemia inhibitory factor.

    abstract::Gp130 is the common signal transducing receptor subunit of interleukin (IL)-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor and cardiotrophin-1. IL-6 and IL-11 induce gp130 homodimerization whereas the others lead to the formation of heterodimers with LIFR or OSMR. Binding e...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Timmermann A,Pflanz S,Grötzinger J,Küster A,Kurth I,Pitard V,Heinrich PC,Müller-Newen G

    更新日期:2000-02-25 00:00:00

  • Hypotonic shocks activate rat TRPV4 in yeast in the absence of polyunsaturated fatty acids.

    abstract::Transient-receptor-potential channels (TRPs) underlie the sensing of chemicals, heat, and mechanical force. We expressed the rat TRPV1 and TRPV4 subtypes in yeast and monitored their activities in vivo as Ca(2+) rise using transgenic aequorin. Heat and capsaicin activate TRPV1 but not TRPV4 in yeast. Hypotonic shocks ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Loukin SH,Su Z,Kung C

    更新日期:2009-02-18 00:00:00

  • Phosphorylation of high- and low-molecular-mass atrial natriuretic peptide analogs by cyclic AMP-dependent protein kinase.

    abstract::Synthetic high- and low-molecular-mass atrial peptides were phosphorylated in vitro by cyclic AMP-dependent protein kinase and [32P]ATP. From a series of atrial peptide analogs, it was deduced that the amino acid sequence, Arg101-Ser104 of atriopeptin was required for optimal phosphorylation. Phosphorylated AP(99-126)...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Olins GM,Mehta PP,Blehm DJ,Patton DR,Zupec ME,Whipple DE,Tjoeng FS,Adams SP,Olins PO,Gierse JK

    更新日期:1987-11-30 00:00:00

  • Search for a dextransucrase minimal motif involved in dextran binding.

    abstract::Fourteen truncated forms of Leuconostoc mesenteroides NRRL B512-F dextransucrase, involving N-, C- or N- plus C-terminal domain truncations were tested for their ability to bind dextrans. The shortest fragment (14kDa molecular weight) that still exhibited a strong interaction with dextran was localized between amino a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Suwannarangsee S,Moulis C,Potocki-Veronese G,Monsan P,Remaud-Simeon M,Chulalaksananukul W

    更新日期:2007-10-02 00:00:00

  • The cytochrome bc1 complex as an antipathogenic target.

    abstract::The cytochrome bc1 complex is a key component of the mitochondrial respiratory chains of many eukaryotic microorganisms that are pathogenic for plants or humans, such as fungi responsible for crop diseases and Plasmodium falciparum, which causes human malaria. Cytochrome bc1 is an enzyme that contains two (ubi)quinone...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Fisher N,Meunier B,Biagini GA

    更新日期:2020-09-01 00:00:00

  • Hsa-miR-125b suppresses bladder cancer development by down-regulating oncogene SIRT7 and oncogenic long non-coding RNA MALAT1.

    abstract::MicroRNAs mainly inhibit coding genes and long non-coding RNA expression. Here, we report that hsa-miR-125b and oncogene SIRT7/oncogenic long non-coding RNA MALAT1 were inversely expressed in bladder cancer. Hsa-miR-125b mimic down-regulated, whereas hsa-miR-125b inhibitor up-regulated the expression of SIRT7 and MALA...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Han Y,Liu Y,Zhang H,Wang T,Diao R,Jiang Z,Gui Y,Cai Z

    更新日期:2013-11-29 00:00:00

  • Ca2+-modulated ONE-GC odorant signal transduction.

    abstract::In a subset of olfactory epithelium the odorant receptor guanylate cyclase, ONE-GC, is a central transduction component of the cyclic GMP signaling pathway. The odorant binds to the extracellular domain and activates its intracellular catalytic domain to generate the odorant second messenger, cyclic GMP. The present s...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Duda T,Sharma RK

    更新日期:2009-04-17 00:00:00

  • Synthesis and maturation of recombinant human tumor necrosis factor in eukaryotic systems.

    abstract::The biosynthesis of human tumor necrosis factor (hTNF) was studied. The amino-terminal extension of the hTNF precursor (26 kDa polypeptide) was not cleaved off in a cell-free system supplemented with dog pancreas microsomes. Correct maturation of pre-hTNF was nevertheless not restricted to the macrophage system: in th...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Müller R,Marmenout A,Fiers W

    更新日期:1986-03-03 00:00:00

  • Spectroscopic characterization of PS I core complexes from thermophilic Synechococcus sp. Identical reoxidation kinetics of A1- before and after removal of the iron-sulfur-clusters FA and FB.

    abstract::Monomeric and trimeric PS I complexes missing the three stromal subunits E,C and D (termed PS I core complexes) were prepared from the thermophilic cyanobacterium Synechococcus sp. by incubation with urea. The subunits E,C and D are sequentially removed. In the monomeric PS I the subunit C is removed with a half life ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lüneberg J,Fromme P,Jekow P,Schlodder E

    更新日期:1994-01-31 00:00:00

  • Reconstruction of tRNAPhe molecules from the fragments by linkage with T-4 RNA ligase in double-stranded regions.

    abstract::Phenylalanine-specific tRNA from yeast was hydrolysed with cobra venom ribonuclease in the double-stranded regions and the fragments isolated. The 'dissected' molecules with nicks in positions 28 and 41 were reconstructed from supplementary fragments and treated with T-4 RNA ligase. A phosphodiester bond between two f...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Boutorin AS,Vassilenko SK,Baklanov MM,Nechaev YS

    更新日期:1984-01-02 00:00:00

  • The RacGAP protein FilGAP is a negative regulator of chemokine-promoted lymphocyte migration.

    abstract::Rho family small GTPases regulate lymphocyte migration induced by chemokines. However, how lymphocyte migration is regulated by Rho GTPases remains to be elucidated. Here, we identified FilGAP, a Rac-specific GAP, as a negative regulator of lymphocyte polarization and migration. Depletion of FilGAP in mouse pro-B BAF ...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Iida T,Saito K,Katagiri K,Kinashi T,Ohta Y

    更新日期:2016-05-01 00:00:00

  • Double-negative feedback loop between long non-coding RNA TUG1 and miR-145 promotes epithelial to mesenchymal transition and radioresistance in human bladder cancer cells.

    abstract::LncRNAs have a critical role in the regulation of cellular processes such as cancer progression and metastasis. In the present study, we confirmed that TUG1 was overexpressed in bladder cancer tissues and established cell lines. Knockdown of TUG1 inhibited bladder cancer cell metastasis both in vitro and in vivo. Furt...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tan J,Qiu K,Li M,Liang Y

    更新日期:2015-10-07 00:00:00

  • Brown adipocytes are novel sites of expression and regulation of adiponectin and resistin.

    abstract::Adiponectin and resistin, two recently identified adipocyte-specific secretory factors, are able to modulate insulin actions in target tissues. To investigate their expression and hormonal regulation in brown adipocytes, we used the brown adipocyte cell line T37i, which, beside uncoupling protein expression, secretes ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Viengchareun S,Zennaro MC,Pascual-Le Tallec L,Lombes M

    更新日期:2002-12-18 00:00:00

  • Drug-resistant malaria: molecular mechanisms and implications for public health.

    abstract::Resistance to antimalarial drugs has often threatened malaria elimination efforts and historically has led to the short-term resurgence of malaria incidences and deaths. With concentrated malaria eradication efforts currently underway, monitoring drug resistance in clinical settings complemented by in vitro drug susce...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Petersen I,Eastman R,Lanzer M

    更新日期:2011-06-06 00:00:00

  • Alterations of lipid composition in Friend leukemia cell tumors in mice treated with tumor necrosis factor-alpha.

    abstract::Lipid analyses were carried out on transplantable murine Friend leukemia cell tumors, 6 h after intratumoral administration of tumor necrosis factor-alpha (TNF). The levels of the major phospholipid classes were uniformly decreased to about 70% of control values; free fatty acids were increased to about 170%; diacylgl...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bravo E,Carpinelli G,Proietti E,Belardelli F,Cantafora A,Podo F

    更新日期:1990-01-29 00:00:00

  • Multiplicite de la phosphatase alcaline cerebrale de differentes especes.

    abstract::The alkaline phosphatase of beef, horse, pig and sheep brain have been purified. In each case, the alkaline phosphatase activity can be separated into two or three components by DEAE-cellulose chromatography and by electrophoresis. Although the properties of these enzyme fractions are very similar, there are sufficien...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Brunel C,Cathala G

    更新日期:1970-02-16 00:00:00

  • Purification of a 175-kDa membrane protein, its localization in smooth and cardiac muscles. Interaction with cytoskeletal protein - vinculin.

    abstract::A new 175-kDa membrane protein was isolated from chicken gizzard smooth muscle. Antibodies to 175-kDa protein were used for localization of this protein in smooth and cardiac muscles. In both types of muscle 175-kDa protein was localized near plasma membrane. 175-kDa protein was able to interact specifically with vinc...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Koteliansky VE,Gneushev GN,Belkin AM

    更新日期:1985-03-11 00:00:00

  • Modelling, synthesis and biological activity of a BLV proteinase, made of (only) 116 amino acids.

    abstract::Bovine leukaemia virus (BLV) is the aetiological agent of Leukosis enzootica bovis [Viral Oncology (1980), G. Klein (Ed.) Raven Press, New York, pp. 231-238], a widely spread disease in cattle. BLV is reported as the animal model of human T-cell leukaemia virus (HLTV) which is the causative agent of adult T-cell leuka...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Précigoux G,Geoffre S,Léonard R,Llido S,Dautant A,d'Estaintot BL,Picard P,Ménard A,Guillemain B,Hospital M

    更新日期:1993-07-12 00:00:00

  • The phosphoinositide 3-kinase/Akt pathway is activated by daunorubicin in human acute myeloid leukemia cell lines.

    abstract::Daunorubicin induces apoptosis in myeloid leukemia cells by activation of neutral sphingomyelinase and ceramide generation occurring 4-10 min after daunorubicin addition. We show here that daunorubicin is able to increase the phosphoinositide 3-kinase activity and enhance intracellular phosphoinositide 3-kinase lipid ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Plo I,Bettaïeb A,Payrastre B,Mansat-De Mas V,Bordier C,Rousse A,Kowalski-Chauvel A,Laurent G,Lautier D

    更新日期:1999-06-11 00:00:00

  • Mechanism of o-aminophenol metabolism in human erythrocytes.

    abstract::o-Aminophenol was found to be rapidly metabolized to a brown compound in the presence of purified human oxy- and methemoglobin, coupled with the oxidation and reduction of these hemoglobins by o-aminophenol. The final product of o-aminophenol was identified as 2-aminophenoxazine-3-one, by using spectrophotometry and H...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tomoda A,Yamaguchi J,Kojima H,Amemiya H,Yoneyama Y

    更新日期:1986-02-03 00:00:00

  • Glycinebetaine enhances and stabilizes the evolution of oxygen and the synthesis of ATP by cyanobacterial thylakoid membranes.

    abstract::Glycinebetaine (betaine), an osmoregulant in halophilic plants, stabilized the evolution of oxygen and the synthesis of ATP by thylakoid membranes from the cyanobacterium Synechocystis PCC6803 when it was present during the preparation and incubation of the thylakoid membranes. Moreover, betaine enhanced the evolution...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mamedov MD,Hayashi H,Wada H,Mohanty PS,Papageorgiou GC,Murata N

    更新日期:1991-12-09 00:00:00

  • Brachypodium distachyon triphosphate tunnel metalloenzyme 3 is both a triphosphatase and an adenylyl cyclase upregulated by mechanical wounding.

    abstract::Proteins with a CyaB, thiamine triphosphatase domain (CYTH domain) may play a central role at the interface between nucleotide and polyphosphate metabolism. One of the plant CYTH domain-containing proteins from Brachypodium distachyon, BdTTM3, is annotated in NCBI databases as an 'adenylyl cyclase (AC)' or a 'triphosp...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Świeżawska B,Duszyn M,Kwiatkowski M,Jaworski K,Pawełek A,Szmidt-Jaworska A

    更新日期:2020-03-01 00:00:00

  • Biochemical identification of the catalytic residues of a glycoside hydrolase family 120 β-xylosidase, involved in xylooligosaccharide metabolisation by gut bacteria.

    abstract::The β-xylosidase B from Bifidobacterium adolescentis ATCC15703 belongs to the newly characterized family 120 of glycoside hydrolases. In order to investigate its catalytic mechanism, an extensive kinetic study of the wild-type enzyme and mutants targeting the three highly conserved residues Asp(393), Glu(416) and Glu(...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cecchini DA,Fauré R,Laville E,Potocki-Veronese G

    更新日期:2015-10-07 00:00:00

  • Cloning and functional expression of a tetrabenazine sensitive vesicular monoamine transporter from bovine chromaffin granules.

    abstract::Using oligonucleotide primers derived from the vesicular monoamine transporters sequences, a cDNA predicted to encode the bovine chromaffin granule amine transporter has been cloned (b-VMAT2). Surprisingly, its structure is more similar to the rat brain transporter (VMAT2), than to the rat adrenal counterpart (VMAT1)....

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Howell M,Shirvan A,Stern-Bach Y,Steiner-Mordoch S,Strasser JE,Dean GE,Schuldiner S

    更新日期:1994-01-24 00:00:00

  • Uric acid substantially enhances the free radical-induced inactivation of alcohol dehydrogenase.

    abstract::Lactate dehydrogenase (LDH) and yeast alcohol dehydrogenase ( YADH ) are inactivated when attacked by hydroxy free radicals (OH). Organic molecules with a high rate constant of reaction with OH such as ascorbate or urate can compete with the enzymes for these strongly oxidising radicals. However, although 10(-3)M asco...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kittridge KJ,Willson RL

    更新日期:1984-05-07 00:00:00

  • Structural changes in profilin accompany its binding to phosphatidylinositol, 4,5-bisphosphate.

    abstract::The effect on the structure of profilin of phosphatidylinositol 4,5-bisphosphate (PIP2) binding was probed by fluorescence and circular dichroism (CD) spectroscopy. Fluorescence of Trp3 and Trp31 of profilin at 292 nm showed a linear decrease in solution emission at 340 nm as PIP2/profilin was increased from 0 to 80:1...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Raghunathan V,Mowery P,Rozycki M,Lindberg U,Schutt C

    更新日期:1992-02-03 00:00:00