Abstract:
:Proteins involved in protein translocation across the membrane of the endoplasmic reticulum assemble into different oligomeric complexes depending on their state of function. To analyse such membrane protein complexes we fractionated proteins of mammalian rough microsomes and analysed them using blue native PAGE and immunoblotting. Among the proteins characterised are the Sec61p complex, the oligosaccharyl transferase (OST) complex, the translocon-associated protein (TRAP) complex, the TRAM and RAMP4 proteins, the signal recognition particle (SRP) and the SRP receptor (SR). Interestingly, the RAMP4 protein, SR and OST complex display more than one oligomeric form.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Wang L,Dobberstein Bdoi
10.1016/s0014-5793(99)01075-3keywords:
subject
Has Abstractpub_date
1999-09-03 00:00:00pages
316-22issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(99)01075-3journal_volume
457pub_type
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