Catechin derivatives: specific inhibitor for caspases-3, 7 and 2, and the prevention of apoptosis at the cell and animal levels.


:Tea-catechin derivatives are shown to inhibit activities of caspases-3, 2 and 7 in vitro, and prevented experimental apoptosis at the cell and animal levels. Epigallo-catechin-gallate showed the strongest inhibition at 1 x 10(-7)M to these caspases, but cysteine cathepsins and caspase-8 were not inhibited. Caspase-3 inhibition showed a 2nd-order allosteric-type, but the inhibition of caspases-2 and 7 showed a non-competitive-type. The apoptosis-test using cultured HeLa cells was inhibited by these catechins. In rat hepatocytes, apoptosis was induced by d-galactosamine in vivo. In this case, caspase-3 activity in the cytoplasm, the serum aminotransferases and dUTP nick formation detected by TUNNEL-staining were effects, and these elevations were suppressed by administration of catechin.




FEBS letters


Katunuma N,Ohashi A,Sano E,Ishimaru N,Hayashi Y,Murata E





Has Abstract


2006-02-06 00:00:00














  • The KDEL receptor: new functions for an old protein.

    abstract::The KDEL receptor is a seven-transmembrane-domain protein that was first described about 20 years ago. Its well-known function is to retrotransport chaperones from the Golgi complex to the endoplasmic reticulum. Recent studies, however, have suggested that the KDEL receptor has additional functions. Indeed, we have de...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Capitani M,Sallese M

    更新日期:2009-12-03 00:00:00

  • Fertilization of Xenopus eggs imposes a complete translational arrest of mRNAs containing 3'UUAUUUAU elements.

    abstract::The early embryonic development of Xenopus is mainly governed by post-transcriptional regulations until the mid-blastula transition. In this report, we present evidence demonstrating that fertilization of Xenopus eggs triggers a complete translational arrest of mRNAs containing UA-rich elements in their 3'-untranslate...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Marinx O,Bertrand S,Karsenti E,Huez G,Kruys V

    更新日期:1994-05-30 00:00:00

  • D-Aspartate stimulation of testosterone synthesis in rat Leydig cells.

    abstract::D-Aspartate increases human chorionic gonadotropin-induced testosterone production in purified rat Leydig cells. L-Aspartate, D-,L-glutamate or D-,L-asparagine could not substitute for D-aspartate and this effect was independent of glutamate receptor activation. Testosterone production was enhanced only in cells cultu...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nagata Y,Homma H,Lee JA,Imai K

    更新日期:1999-02-12 00:00:00

  • Bacterial proteins carrying twin-R signal peptides are specifically targeted by the delta pH-dependent transport machinery of the thylakoid membrane system.

    abstract::Glucose-fructose oxidoreductase (GFOR), a periplasmic protein of Zymomonas mobilis, is synthesized as a precursor polypeptide with a twin-R signal peptide for Sec-independent protein export in bacteria. In higher plant chloroplasts, twin-R signal peptides are specific targeting signals for the Sec-independent delta pH...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Halbig D,Hou B,Freudl R,Sprenger GA,Klösgen RB

    更新日期:1999-03-19 00:00:00

  • Cysteine is not a substrate but a specific modulator of human ASCT2 (SLC1A5) transporter.

    abstract::The Alanine Serine Cysteine Transporter 2 (ASCT2) is involved in balancing the intracellular amino acid pool. This function is allowed by the antiport mechanism and the asymmetric specificity towards different neutral amino acids, distinctive of this transporter. In the present work, the interaction of the putative su...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Scalise M,Pochini L,Pingitore P,Hedfalk K,Indiveri C

    更新日期:2015-11-30 00:00:00

  • Human HRD1 protects against ER stress-induced apoptosis through ER-associated degradation.

    abstract::Stresses that impair the function of the endoplasmic reticulum (ER) lead to an accumulation of unfolded protein in the ER. Under these conditions, the expression of a variety of genes involved in preventing the accumulation of the unfolded proteins is induced. Yeast Hrd1p is an ER stress-inducible ER membrane protein ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kaneko M,Ishiguro M,Niinuma Y,Uesugi M,Nomura Y

    更新日期:2002-12-04 00:00:00

  • Insulin-like growth factor I supports differentiation of cultured osteoblast-like cells.

    abstract::Rat calvaria cells grown in culture for one week had properties of osteoblasts: a high content in alkaline phosphatase and a marked cyclic AMP response to parathyroid hormone (PTH). In short-term experiments, insulin-like growth factor I (IGF I) stimulated the incorporation of [14C] glucose into glycogen. When IGF I w...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Schmid C,Steiner T,Froesch ER

    更新日期:1984-07-23 00:00:00

  • Inhibition of the mitochondrial cyclosporin A-sensitive permeability transition pore by the arginine reagent phenylglyoxal.

    abstract::The mitochondrial permeability transition pore, a cyclosporin A-sensitive channel, is controlled by the transmembrane electric potential difference across the inner membrane. Here, we show that treatment of rat liver mitochondria with the arginine reagent phenylglyoxal inhibits the permeability transition pore trigger...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Eriksson O,Fontaine E,Petronilli V,Bernardi P

    更新日期:1997-06-16 00:00:00

  • Do the major human glutathione S-transferases have fatty acid ethyl ester synthase activity?

    abstract::Two fatty acid ethyl ester (FAEE) synthase isoenzymes purified from human myocardium were reported to be glutathione S-transferases (GST) [(1989) Proc. Natl. Acad. Sci. USA 86, 4470-4473; and (1989) J. Clin. Invest. 84, 1942-1946]. In the present study, the FAEE synthase activity of several purified and well character...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Suzuki T,Kovacs MS,Board PG

    更新日期:1990-11-26 00:00:00

  • Primary structure of a multimeric protein, homologous to the PEP-utilizing enzyme family and isolated from a hyperthermophilic archaebacterium.

    abstract::A large protein complex (approx. 2000 kDa) was found in the cytosol of the hyperthermophilic archaebacterium Staphylothermus marinus. The purified protein was shown to be a homomultimer of 93 kDa subunits, the primary structure of which was determined by nucleotide sequence analysis. The protein belongs to the family ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cicicopol C,Peters J,Kellermann J,Baumeister W

    更新日期:1994-12-19 00:00:00

  • Function of nucleophosmin/B23, a nucleolar acidic protein, as a histone chaperone.

    abstract::We previously identified and purified a nucleolar phosphoprotein, nucleophosmin/B23, as a stimulatory factor for replication from the adenovirus chromatin. We show here that nucleophosmin/B23 functions as a histone chaperone protein such as nucleoplasmin, TAF-I, and NAP-I. Nucleophosmin/B23 was shown to bind to histon...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Okuwaki M,Matsumoto K,Tsujimoto M,Nagata K

    更新日期:2001-10-12 00:00:00

  • Phosphorylation of plant actin-depolymerising factor by calmodulin-like domain protein kinase.

    abstract::The actin-depolymerising factor (ADF)/cofilin group of proteins are stimulus-responsive actin-severing proteins, members of which are regulated by reversible phosphorylation. The phosphorylation site on the maize ADF, ZmADF3, is Ser-6 but the kinase responsible is unknown [Smertenko et al., Plant J. 14 (1998) 187-193]...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Allwood EG,Smertenko AP,Hussey PJ

    更新日期:2001-06-15 00:00:00

  • Heparin promotes fibril formation by the N-terminal fragment of amyloidogenic apolipoprotein A-I.

    abstract::Glycosaminoglycans are known to be associated with extracellular amyloid deposits of various amyloidogenic proteins. In this study, we found that the glycosaminoglycan heparin greatly accelerates the elongation step in fibril formation by the N-terminal 1-83 fragment of human apolipoprotein A-I (apoA-I), especially in...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Mikawa S,Mizuguchi C,Nishitsuji K,Baba T,Shigenaga A,Shimanouchi T,Sakashita N,Otaka A,Akaji K,Saito H

    更新日期:2016-10-01 00:00:00

  • In vitro dissociation of self-assembly of three chaperonin 60s: the role of ATP.

    abstract::A comparative study has investigated the in vitro dissociation and self-assembly of chaperonin 60 14-mers isolated from E. coli (GroEL), yeast mitochondria and pea chloroplasts. In all cases Mg2+ inhibits, and low temperature stimulates, the urea-induced dissociation. ATP or ADP in the presence of Mg2+ enhance the dis...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lissin NM

    更新日期:1995-03-13 00:00:00

  • Structure-dependent functional properties of human defensin 5.

    abstract::The mucosal epithelium secretes a variety of antimicrobial peptides that act as part of the innate immune system to protect against invading microbes. Here, we describe the functional properties of human defensin (HD) 5, the major antimicrobial peptide produced by Paneth cells in the ileum, in relation to its structur...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: de Leeuw E,Burks SR,Li X,Kao JP,Lu W

    更新日期:2007-02-06 00:00:00

  • SABP2, a methyl salicylate esterase is required for the systemic acquired resistance induced by acibenzolar-S-methyl in plants.

    abstract::Tobacco SABP2, a 29kDa protein catalyzes the conversion of methyl salicylic acid (MeSA) into salicylic acid (SA) to induce SAR. Pretreatment of plants with acibenzolar-S-methyl (ASM), a functional analog of salicylic acid induces systemic acquired resistance (SAR). Data presented in this paper suggest that SABP2 catal...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tripathi D,Jiang YL,Kumar D

    更新日期:2010-08-04 00:00:00

  • Insulin processing and action in adipocytes: evidence for generation of insulin-containing vesicles by leupeptin and monensin.

    abstract::Incubation of adipocytes with 125I-insulin plus leupeptin or monensin, but not chloroquine, resulted in the appearance of a novel peak of 125I-insulin (modal density about 1.20 g/ml) on density gradient centrifugation; the appearance of the peak depended on the presence of specific insulin receptors on the cell surfac...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Jeffrey IW,Flint DJ,Vernon RG,Mayer RJ

    更新日期:1985-03-25 00:00:00

  • The inhibitory effect of nitrite, a stable product of nitric oxide (NO) formation, on arginase.

    abstract::Macrophages contain arginase and an inducible NO synthase, demonstrated by using L-arginine, the common substrate, for production of both nitric oxide and urea. Arginase was inhibited by nitrite, the stable end product of NO. This inhibition was non-competitive, and could not be explained by the reaction of nitrite wi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hrabák A,Bajor T,Temesi A,Mészáros G

    更新日期:1996-07-22 00:00:00

  • Characterization of energetically functional inverted membrane vesicles from Corynebacterium glutamicum.

    abstract::We show that inverted membrane vesicles from Corynebacterium glutamicum, a Gram-positive bacterium, are able to generate and maintain an electrochemical gradient of protons in response to the addition of NADH. This result indicates that the respiratory chain is intact and that the vesicles are reasonably impermeable t...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Schrempp S,Bayan N,Shechter E

    更新日期:1994-12-12 00:00:00

  • HIV-1 envelope protein, gp120, has no effects on inositol phosphate production and metabolism in the Jurkat T-cell line either in the presence or absence of receptor stimulation.

    abstract::We have used HPLC techniques to investigate the effects of gp120 upon inositol phosphate turnover in Jurkat E6-1 CD4+ T-cells, to pursue previous reports that this viral coat protein: (a) inhibits receptor-activated inositol phosphate release; (b) stimulates basal inositol phosphate release; (c) inhibits inositol poly...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sumner MT,Shears SB

    更新日期:1997-08-11 00:00:00

  • Structural variability of the ubiquitin specific protease DUSP-UBL double domains.

    abstract::USP4, 11 and 15 are three closely related paralogues of the ubiquitin specific protease (USP) family of deubiquitinating enzymes. The DUSP domain and the UBL domain in these proteins are juxtaposed which may provide a functional unit conferring specificity. We determined the structures of the USP15 DUSP-UBL double dom...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Elliott PR,Liu H,Pastok MW,Grossmann GJ,Rigden DJ,Clague MJ,Urbé S,Barsukov IL

    更新日期:2011-11-04 00:00:00

  • Cellulysin from the plant parasitic fungus Trichoderma viride elicits volatile biosynthesis in higher plants via the octadecanoid signalling cascade.

    abstract::Cellulysin, a crude cellulase from the plant parasitic fungus Trichoderma viride, induces the biosynthesis of volatiles in higher plants (Nicotiana plumbaginifolia, Phaseolus lunatus, and Zea mays) when applied to cut petioles by the transpiration stream. The pattern of the emitted volatiles largely resembles that fro...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Piel J,Atzorn R,Gäbler R,Kühnemann F,Boland W

    更新日期:1997-10-20 00:00:00

  • Effects of orexin A on proliferation, survival, apoptosis and differentiation of 3T3-L1 preadipocytes into mature adipocytes.

    abstract::Metabolic activities of orexin A (OXA) in mature adipocytes are mediated via PI3K/PKB and PPARγ. However, the effects of OXA on preadipocytes are largely unknown. We report here that OXA stimulates the proliferation and viability of 3T3-L1 preadipocytes and protects them from apoptosis via ERK1/2, but not through PKB....

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Skrzypski M,Kaczmarek P,Le TT,Wojciechowicz T,Pruszyńska-Oszmalek E,Szczepankiewicz D,Sassek M,Arafat A,Wiedenmann B,Nowak KW,Strowski MZ

    更新日期:2012-11-30 00:00:00

  • Expression of human apolipoprotein A-I/C-III/A-IV gene cluster in mice reduces atherogenesis in response to a high fat-high cholesterol diet.

    abstract::We have previously generated transgenic (Tg) mice expressing the human apolipoprotein (apo) A-I/C-III/A-IV gene cluster. This expression induced hyperlipidemia but reduced atherosclerotic lesions in genetically modified mice lacking apoE. Atherosclerosis is a multifactorial process and environmental factors such as di...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Baroukh N,Ostos MA,Vergnes L,Recalde D,Staels B,Fruchart J,Ochoa A,Castro G,Zakin MM

    更新日期:2001-07-27 00:00:00

  • A preferential pole for exocytosis in cultured chromaffin cells revealed by confocal microscopy.

    abstract::Histological studies suggest that adrenal medulla chromaffin cells in situ are polarised, but functional evidence is lacking. We present here the first demonstration for polarisation of exocytosis in isolated, spherical, bovine chromaffin cells. Cells were stimulated with 70 mM K(+) to cause a marked enhancement of ca...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cuchillo-Ibáñez I,Michelena P,Albillos A,García AG

    更新日期:1999-10-01 00:00:00

  • The apical sorting signal on human aminopeptidase N is not located in the stalk but in the catalytic head group.

    abstract::Human aminopeptidase N carries an apical sorting signal on its ectodomain necessary for its correct transport to the apical membrane in Madin-Darby canine kidney cells. To determine whether the apical sorting signal is localized in the serine/threonine rich stalk or in the catalytic head group, anchor/stalk-minus amin...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Vogel LK,Norén O,Sjöström H

    更新日期:1992-08-10 00:00:00

  • Wortmannin and 1-butanol block activation of a novel family of protein kinases in neutrophils.

    abstract::Neutrophils contain four uncharacterized protein kinases with molecular masses of ca. 69, 63, 49 and 40 kDa that are rapidly activated upon stimulation of these cells with the chemoattractant fMet-Leu-Phe [Ding, J. and Badwey, J.A. (1993) J. Biol. Chem. 268, 17326-17333]. We now report that wortmannin and 1-butanol bl...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ding J,Badwey JA

    更新日期:1994-07-11 00:00:00

  • Bcl-2 and Bax are involved in the sympathetic protection of brown adipocytes from obesity-linked apoptosis.

    abstract::Obesity is linked to functional brown adipose tissue (BAT) atrophy, partially due to adipocyte apoptosis. The brown adipocytes of obese rats have lower Bcl-2/Bax mRNA and protein ratios than those of their lean littermates. Exposure to a low temperature for three days markedly increased the Bcl-2/Bax ratio, by increas...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Briscini L,Tonello C,Dioni L,Carruba MO,Nisoli E

    更新日期:1998-07-10 00:00:00

  • Ca2+-modulated ONE-GC odorant signal transduction.

    abstract::In a subset of olfactory epithelium the odorant receptor guanylate cyclase, ONE-GC, is a central transduction component of the cyclic GMP signaling pathway. The odorant binds to the extracellular domain and activates its intracellular catalytic domain to generate the odorant second messenger, cyclic GMP. The present s...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Duda T,Sharma RK

    更新日期:2009-04-17 00:00:00

  • The Arabidopsis thaliana trehalase is a plasma membrane-bound enzyme with extracellular activity.

    abstract::The lack of trehalose accumulation in most plant species has been partly attributed to the presence of an active trehalase. Although trehalose synthesis enzymes are thought to be cytosolic, and previous studies have indicated that trehalase activity is extracellular, the exact location of the enzyme has not yet been e...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Frison M,Parrou JL,Guillaumot D,Masquelier D,François J,Chaumont F,Batoko H

    更新日期:2007-08-21 00:00:00