Abstract:
:The aim of the present study was to investigate homoharringtonine alkaloid effect on: (i) the nonenzymatic and eEF-1-dependent Phe-tRNAPhe binding to poly(U)-programmed human placenta 80 S ribosomes; (ii) diphenylalanine synthesis accompanying nonenzymatic Phe-tRNAPhe binding; and (iii) acetylphenylalanyl-puromycin formation. Neither nonenzymatic nor eEF-1-dependent Phe-tRNAPhe binding were noticeably affected by the alkaloid, whereas diphenylalanine synthesis and puromycin reaction were strongly inhibited by homoharringtonine. It has been proposed that the site of homoharringtonine binding on 80 S ribosomes should overlap or coincide with the acceptor site of the ribosome.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Tujebajeva RM,Graifer DM,Karpova GG,Ajtkhozhina NAdoi
10.1016/0014-5793(89)81546-7subject
Has Abstractpub_date
1989-11-06 00:00:00pages
254-6issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(89)81546-7journal_volume
257pub_type
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