Abstract:
:The biological activity and the fate of SV40 DNA (minichromosomes, DNA I, DNA II, DNA III) were tested in culture cells by immunofluorescence staining and blot analysis. Following microinjection of 2-4 circular SV40 molecules (minichromosomes, DNA I, DNA II) into the cytoplasm or the nuclei of monkey and rat cells, T- and V-antigen synthesis was demonstrable in nearly every recipient cell. Only linear DNA induced T-antigen synthesis with a very low efficiency after cytoplasmic injection. This low activity correlates with a rapid degradation of DNA III in the recipient cells. Further modifications observed immediately after injection are relaxation of superhelical molecules and formation of high-Mr DNA. Assembly of the injected DNA into SV40 chromatin-like structure, however, occurred only late after early viral gene expression.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Graessmann A,Bumke-Vogt C,Buschhausen G,Bauer M,Graessmann Mdoi
10.1016/0014-5793(85)80187-3subject
Has Abstractpub_date
1985-01-01 00:00:00pages
41-5issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(85)80187-3journal_volume
179pub_type
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