4E-BP3 regulates eIF4E-mediated nuclear mRNA export and interacts with replication protein A2.

abstract：:The retinal pigment epithelium-specific 65 kDa (RPE65) isomerase plays a pivotal role in photoreceptor survival and function. RPE65-catalyzed synthesis of 11-cis-retinol from all-trans-retinyl esters in the visual cycle is negatively regulated, through a heretofore unknown mechanism, by the fatty acid transport protei...

journal_title：FEBS letters

authors： Li S,Green JF,Jin M

更新日期：2020-02-01 00:00:00

abstract：:Genetically obese (ob/ob) mice develop a marked insulin resistance in brown adipose tissue soon after weaning, and this is paralleled by a fall in the acute activation of the mitochondrial proton conductance pathway in the tissue on cold exposure. Treatment of ob/ob mice with ciglitazone, a new oral hypoglycaemic, led...

journal_title：FEBS letters

authors： Mercer SW,Trayhurn P

更新日期：1986-01-20 00:00:00

abstract：:Hydrogenase gene from Clostridium butyricum was cloned in Escherichia coli HK16 (Hyd-) using pBR322 and PstI. The plasmid, pCBH1, containing hydrogenase gene was 7.3 MDa and pCBH1 had 5 PstI-DNA fragments (3.9, 2.6, 0.7, 0.03-0.04, less than 0.02 MDa, respectively). The hydrogenase activity of HK16 (pCBH1) was about 3...

journal_title：FEBS letters

authors： Karube I,Urano N,Yamada T,Hirochika H,Sakaguchi K

更新日期：1983-07-11 00:00:00

abstract：:The nuclear magnetic resonance solution structure of alpha-conotoxin SI has been determined at pH 4.2. The 36 lowest energy structures show that alpha-conotoxin SI exists in a single major solution conformation and is stabilized by six hydrogen bonds. Comparisons are made between the SI solution structure and the solu...

journal_title：FEBS letters

authors： Benie AJ,Whitford D,Hargittai B,Barany G,Janes RW

更新日期：2000-07-07 00:00:00

abstract：:Bisdioxopiperazine anti-cancer agents are catalytic inhibitors of topoisomerase II which by unknown means lock the enzyme in a closed clamp form and inhibit its ATPase activity. In order to demarcate a putative pharmacophore, we here describe a novel Tyr165Ser mutation in the enzyme's Walker A ATP binding site leading...

journal_title：FEBS letters

authors： Wessel I,Jensen LH,Renodon-Corniere A,Sorensen TK,Nitiss JL,Jensen PB,Sehested M

更新日期：2002-06-05 00:00:00

abstract：:A central step in xanthone biosynthesis is the formation of the C13 skeleton, i.e. an intermediate benzophenone. Biosynthesis of 2,3',4,6-tetrahydroxybenzophenone from m-hydroxybenzoyl-CoA and malonyl-CoA was shown in cell-free extracts from cultured cells of Centaurium erythraea. The enzyme catalyzing this reaction w...

journal_title：FEBS letters

authors： Beerhues L

更新日期：1996-04-01 00:00:00

abstract：:The purinergic P2X(7) receptor is an ATP-receptor channel predominantly expressed in immune cells. P2X(7) has been cloned from human, rat and mouse. Here we report cloning of the Xenopus laevis P2X(7) receptor (xP2X(7)). xP2X(7) is only about 50% identical to the mammalian homologues, shows a broad tissue expression p...

journal_title：FEBS letters

authors： Paukert M,Hidayat S,Gründer S

更新日期：2002-02-27 00:00:00

abstract：:The possible in vivo interaction of the Nicotiana tabacum agglutinin (Nictaba) with endogenous glycoproteins was corroborated using a combination of confocal/electron microscopy of an EGFP-Nictaba fusion protein expressed in tobacco Bright Yellow-2 (BY-2) cells and biochemical analyses. In vitro binding studies demons...

journal_title：FEBS letters

authors： Lannoo N,Peumans WJ,Pamel EV,Alvarez R,Xiong TC,Hause G,Mazars C,Van Damme EJ

更新日期：2006-11-27 00:00:00

abstract：:Platinum electrodes can be coated with cytochrome b5-phospholipid monolayers by the Langmuir-Blodgett technique. Cyclic voltammetry of a series of dyes shows that the coated electrodes become selective for certain electroactive species. The electron transfer reactions of negatively charged species are inhibited at the...

journal_title：FEBS letters

authors： Zaba BN,Wilkinson MC,Taylor DM,Lewis TJ,Laidman DL

更新日期：1987-03-09 00:00:00

abstract：:Acceptor stem variants of tRNA(fMet) (Escherichia coli) have been characterized by nuclear magnetic resonance. The wild type contains a C1-A72 mismatch pair which is crucial for its biological function. For comparison, the mismatch was replaced by regular pairs U1-A72 and C1-G72. Further variants contain an altered di...

journal_title：FEBS letters

authors： Zuleeg T,Vogtherr M,Schübel H,Limmer S

更新日期：2000-04-28 00:00:00

abstract：:alpha-1-Antitrypsin is found in hepatocytes as a high-mannose glycoprotein (Mr 49 000), extracellularly as a complex-type glycoprotein (Mr 54 000). Deglycosylation of both forms with peptide: N-glycosidase led to proteins of identical app. Mr (41 000). The sequence of 26 N-terminal amino acids of rat alpha 1-antitryps...

journal_title：FEBS letters

authors： Gross V,Kaiser C,Tran-Thi TA,Schmelzer E,Witt I,Plummer TH Jr,Heinrich PC

更新日期：1983-01-24 00:00:00

abstract：:Glutathione peroxidase 3 (GPX3) is an important member of antioxidant enzymes for reducing reactive oxygen species and maintaining the oxygen balance. Gpx3 mRNA is strongly expressed in decidual cells from days 5 to 8 of pregnancy. After pregnant mice are treated with GPX inhibitor for 3 days, pregnancy rate is signif...

journal_title：FEBS letters

authors： Xu X,Leng JY,Gao F,Zhao ZA,Deng WB,Liang XH,Zhang YJ,Zhang ZR,Li M,Sha AG,Yang ZM

更新日期：2014-05-02 00:00:00

abstract：:Methanosarcina barkeri inserts pyrrolysine (Pyl) at an in-frame UAG codon in its monomethylamine methyltransferase gene. Pyrrolysyl-tRNA synthetase acylates Pyl onto tRNAPyl, the amber suppressor pyrrolysine Pyl tRNA. Here we show that M. barkeri Fusaro tRNAPyl can be misacylated with serine by the M. barkeri bacteria...

journal_title：FEBS letters

authors： Gundllapalli S,Ambrogelly A,Umehara T,Li D,Polycarpo C,Söll D

更新日期：2008-10-15 00:00:00

abstract：:Designed mutations within the Streptomyces lividans kcsA gene resulted in a set of mutant proteins, which were characterized in respect to their assembly and channel activities. (i) The amino acid residue leucine 81 located at the external side of KcsA was found to be exchangeable by a cysteine residue without affecti...

journal_title：FEBS letters

authors： Splitt H,Meuser D,Borovok I,Betzler M,Schrempf H

更新日期：2000-04-21 00:00:00

abstract：:The positive strand RNA coronavirus, infectious bronchitis virus (IBV), induces a G2/M phase arrest and reduction in the G1 and G1/S phase transition regulator cyclin D1. Quantitative real-time RT-PCR and Western blot analysis demonstrated that cyclin D1 was reduced post-transcriptionally within infected cells indepen...

journal_title：FEBS letters

authors： Harrison SM,Dove BK,Rothwell L,Kaiser P,Tarpey I,Brooks G,Hiscox JA

更新日期：2007-04-03 00:00:00

abstract：:Highly purified mitochondria from potato (Solanum tuberosum L. cv. Bintje) tubers were subfractionated into a matrix fraction, an inner membrane fraction and an outer membrane fraction with minimal cross-contamination. When the matrix and inner membrane fractions were incubated with [gamma-32P]ATP only one and three p...

journal_title：FEBS letters

authors： Pical C,Fredlund KM,Petit PX,Sommarin M,Møller IM

更新日期：1993-12-27 00:00:00

abstract：:The mongoose AChR alpha-subunit has been cloned and shown to be highly homologous to other AChR alpha-subunits, with only six differences in amino acid residues at positions that are conserved in animal species that bind alpha-bungarotoxin (alpha-BTX). Four of these six substitutions cluster in the ligand binding site...

journal_title：FEBS letters

authors： Asher O,Jensen BS,Lupu-Meiri M,Oron Y,Fuchs S

更新日期：1998-04-17 00:00:00

abstract：:Ribonucleoprotein complexes, which contain mRNAs and their regulator proteins, carry out post-transcriptional control of gene expression. The function of many RNA-binding proteins depends on their association with cofactors. Here, we use a genomic approach to identify transcripts associated with DLC-1, a protein previ...

journal_title：FEBS letters

authors： Day NJ,Ellenbecker M,Voronina E

更新日期：2018-11-01 00:00:00

abstract：:The effect of neuropeptide Y (NPY) on cytosolic free Ca2+ concentration ([Ca2+]i) was studied in cultured smooth muscle cells from porcine aorta (PASMC) and compared with the effect of bradykinin (BK) and angiotensin II (ATII) on [Ca2+]i. All peptides induced dose-dependent and transient rises in [Ca2+]i which were no...

journal_title：FEBS letters

authors： Mihara S,Shigeri Y,Fujimoto M

更新日期：1989-12-18 00:00:00

abstract：:Using a variety of peptide analogues of oxytocin (OT) and Arg8-vasopressin (AVP), OT-mediated induction of urokinase-type plasminogen activator (uPA) was examined in LLC-PK1 renal epithelial cells, which possess distinct high-affinity receptors of both the OT- and vasopressin renal (V2-) types. OT or OT-receptor speci...

journal_title：FEBS letters

authors： Jans DA,Pavo I,Fahrenholz F

更新日期：1993-01-04 00:00:00

abstract：:The A-rich bulge in paired region P5a of the Tetrahymena intron is a structural feature that is conserved in the sub-group Ib self-splicing introns. We have constructed a series of substitution and deletion mutations in this region of the intron. Kinetic analysis has shown that some of the mutants have a reduced maxim...

journal_title：FEBS letters

authors： Pace U,Szostak JW

更新日期：1991-03-11 00:00:00

abstract：:A photoactive reaction center complex was isolated from a thermophilic green sulfur bacterium, Chlorobium tepidum under anaerobic conditions. The electron transfer occurred from heme c to the photo-oxidized reaction center chlorophyll, P840+, with a half time (t1/2) of 110 or 340 microseconds at 24 or 12 degrees C, re...

journal_title：FEBS letters

authors： Oh-oka H,Kamei S,Matsubara H,Iwaki M,Itoh S

更新日期：1995-05-22 00:00:00

abstract：:Mesenchymal stem cell (MSC) transplantation is used for treatment of many diseases. The paracrine role of MSCs in tissue regeneration is attracting particular attention. We investigate the role of MSC exosomes in skeletal muscle regeneration. MSC exosomes promote myogenesis and angiogenesis in vitro, and muscle regene...

journal_title：FEBS letters

authors： Nakamura Y,Miyaki S,Ishitobi H,Matsuyama S,Nakasa T,Kamei N,Akimoto T,Higashi Y,Ochi M

更新日期：2015-05-08 00:00:00

abstract：:In eukaryotic cells, peroxiredoxins are both antioxidants and regulators of H(2)O(2)-mediated signaling. We previously found that peroxiredoxin-I (Prx-I) was overexpressed in non-small cell lung cancer (NSCLC) tissue. Since overexpressed protein can induce a humoral immune response, we examined whether serum from NSCL...

journal_title：FEBS letters

authors： Chang JW,Lee SH,Jeong JY,Chae HZ,Kim YC,Park ZY,Yoo YJ

更新日期：2005-05-23 00:00:00

abstract：:We studied the molecular binding specificity of two rabbit polyclonal sera generated against phenolic glycolipid antigens namely PheG1 B and PheG1 B-3 from Mycobacterium bovis BCG. PheG1 B is the well-known mycoside B (2-O-Me-alpha-L-Rhap 1----aglycone), while PheG1 B-3 is a recently found glycolipid (alpha-L-Rhap-(1-...

journal_title：FEBS letters

authors： Vercellone A,Riviere M,Fournié JJ,Puzo G

更新日期：1992-05-25 00:00:00

abstract：:The predominance of oxidative stress in many tumour cell environments provides a means to selectively target these cells via protein oxidation. The zinc fingers of transcription factors utilise cysteine thiols for structural zinc coordination. Redox control of DNA binding regulates transcription and therefore the over...

journal_title：FEBS letters

authors： Giles NM,Gutowski NJ,Giles GI,Jacob C

更新日期：2003-01-30 00:00:00

abstract：:Fructose-1,6-bisphosphatase, a key enzyme in gluconeogenesis, undergoes catabolite inactivation when glucose is added to gluconeogenetically active cells of the yeast Saccharomyces cerevisiae. Phosphorylation of the enzyme is followed by rapid degradation. To elucidate the cellular proteolytic system involved in catab...

journal_title：FEBS letters

authors： Schork SM,Bee G,Thumm M,Wolf DH

更新日期：1994-08-01 00:00:00

abstract：:Co-infection with hepatitis B virus (HBV) and hepatitis C virus (HCV) is common and is associated with a more severe liver disease and increased frequency in the development of hepatocellular carcinoma (HCC). Here, we demonstrated that HBV X protein (HBx) and HCV core protein additively repress the universal cyclin-de...

journal_title：FEBS letters

authors： Han HJ,Jung EY,Lee WJ,Jang KL

更新日期：2002-05-08 00:00:00

abstract：:A 1000-bp fragment of Enterococcus hirae genomic DNA was amplified by the polymerase chain reaction method, using the oligonucleotide primers designed from amino acid sequences of both amino-terminal and a tryptic fragment of the Na(+)-ATPase alpha-subunit in this organism. DNA sequencing of this product revealed that...

journal_title：FEBS letters

authors： Kakinuma Y,Igarashi K,Konishi K,Yamato I

更新日期：1991-11-04 00:00:00

abstract：:Stimulation of monocytes/macrophages with lipopolysaccharide (LPS) results in activation of nuclear factor-kappaB (NF-kappaB), which plays crucial roles in regulating expression of many genes involved in the subsequent inflammatory responses. Here, we investigated roles of transforming growth factor-beta activated kin...

journal_title：FEBS letters

authors： Irie T,Muta T,Takeshige K

更新日期：2000-02-11 00:00:00