MicroRNA-320a inhibits cell proliferation, migration and invasion by targeting BMI-1 in nasopharyngeal carcinoma.

abstract：:Bispecific miniantibodies were constructed by genetically fusing the C(H)1 domain of an IgG1 to the C-terminus of a single-chain Fv fragment (scFv-425), specific for the EGF receptor, and fusing the C(L) domain of a kappa light chain to the C-terminus of a scFv specific for CD2 (scFv-M1). An efficient dicistronic gene...

journal_title：FEBS letters

authors： Müller KM,Arndt KM,Strittmatter W,Plückthun A

更新日期：1998-01-30 00:00:00

abstract：:ATP synthesis rate, measured as the variation in external Pi concentration, varied as a linear function of either delta mu H+ or delta Gpin, in such a manner that the delta Gpin/delta mu H+ ratio increased while VATP increased. We also observed a linear dependence of the flux control coefficient of the Pi carrier on...

journal_title：FEBS letters

authors： Beauvoit B,Rigoulet M,Guerin B

更新日期：1989-02-27 00:00:00

abstract：:Effects of amino acid substitutions in the first extracellular loop region of the delta- and mu-opioid receptors were examined. Substitution of lysine-108 of the delta-receptor (delta K108) with asparagine improved affinity to [D-Ala2,MePhe4,Gly-ol5]enk ephalin (DAGO), a mu-selective peptide agonist, to be comparable ...

journal_title：FEBS letters

authors： Fukuda K,Terasako K,Kato S,Mori K

更新日期：1995-10-09 00:00:00

abstract：:We have constructed cDNA clones covering the entire coding region of mouse, human and rabbit preprocathepsin K mRNA for studies on bone turnover. The clone pMCatK-1 for mouse cathepsin K shares 87% nucleotide homology with the corresponding human and rabbit sequences. Analysis of a panel of mouse tissues for tissue di...

journal_title：FEBS letters

authors： Rantakokko J,Aro HT,Savontaus M,Vuorio E

更新日期：1996-09-16 00:00:00

abstract：:An antibody recognising acetylated core histones was used to immunoprecipitate chromatin fragments from proliferating human K562 cells and from cells induced to differentiate with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). The DNA of the acetylated chromatin was probed with sequences of platelet der...

journal_title：FEBS letters

authors： Clayton AL,Hebbes TR,Thorne AW,Crane-Robinson C

更新日期：1993-12-20 00:00:00

abstract：:In the lysosomal storage disorder Gaucher disease (GD), glucosylceramide (GlcCer) accumulates due to the defective activity of glucocerebrosidase. A subset of GD patients develops neuropathology. We now show mislocalization of Limp2-positive puncta and a large reduction in the number of Lamp1-positive puncta, which ar...

journal_title：FEBS letters

authors： Zigdon H,Meshcheriakova A,Farfel-Becker T,Volpert G,Sabanay H,Futerman AH

更新日期：2017-03-01 00:00:00

abstract：:Stimuli which enhance secretion from parathyroid cells such as low extracellular Ca2+ or Mg2+ are associated with a decrease in the cytosolic Ca2+ concentration as measured by quin2. Current evidence suggests that increased production of inositol 1,4,5-triphosphate (IP3) releases Ca2+ from cellular stores thus increas...

journal_title：FEBS letters

authors： Epstein PA,Prentki M,Attie MF

更新日期：1985-08-19 00:00:00

abstract：:We previously have shown that the overexpression of phospholipase C-gamma1 (PLC-gamma1) in rat 3Y1 fibroblasts results in malignant transformation (Chang, J.-S., Noh, D.Y., Park, I.A., Kim, M;.J., Song, H., Ryu, S.H. and Suh, P.-G. (1997) Cancer Res. 57, 5465-5468). The transformed cells, which initially are in an elo...

journal_title：FEBS letters

authors： Chang JS,Iwashita S,Lee YH,Kim MJ,Ryu SH,Suh PG

更新日期：1999-10-22 00:00:00

abstract：:Although decapping is an important process in eukaryotic mRNA turnover, little is known about this process in plants. Here, we identified Arabidopsis thaliana decapping proteins AtDCP1 and AtDCP2 and showed that (I) AtDCP2 is an active decapping enzyme, (II) AtDCP1 interacts with itself, (III) AtDCP1 and AtDCP2 are lo...

journal_title：FEBS letters

authors： Iwasaki S,Takeda A,Motose H,Watanabe Y

更新日期：2007-05-29 00:00:00

abstract：:Crigler-Najjar (CN) disease is caused by a deficiency of the hepatic enzyme, bilirubin UDP-glucuronosyltransferase (B-UGT). We have found two CN type II patients, who were homozygous for a leucine to arginine transition at position 15 of B-UGT1. This mutation is expected to disrupt the hydrophobic core of the signal p...

journal_title：FEBS letters

authors： Seppen J,Steenken E,Lindhout D,Bosma PJ,Elferink RP

更新日期：1996-07-29 00:00:00

abstract：:In vitro cultured blood cells for transfusion purposes provide a safe alternative to donor blood, particularly for patients who require recurrent transfusions, and can be used as carriers of therapeutic molecules. In vitro derivation of hematopoietic cell types from human-induced pluripotent stem cells (iPSCs) allows ...

journal_title：FEBS letters

authors： Hansen M,von Lindern M,van den Akker E,Varga E

更新日期：2019-12-01 00:00:00

abstract：:Most tumor cells attain their immortality by reactivating telomerase. We report here the telomerase inhibitory potential of chimeric oligonucleotides composed of a 13mer antisense sequence targeting the telomerase RNA template region and a (s4dU)n moiety at its 3' or 5'-end. The increase of the thiolated chain length ...

journal_title：FEBS letters

authors： Tarkanyi I,Horváth A,Szatmari I,Eizert H,Vámosi G,Damjanovich S,Ségal-Bendirdjian E,Aradi J

更新日期：2005-02-28 00:00:00

abstract：:The catalytic cycle of heme peroxidases involves two reactive states, compound I and compound II. Although their reduction potentials at pH 7 are similar, compound I is in general more reactive towards organic substrates than compound II. The different reactivities have until now remained unexplained. In this study, t...

journal_title：FEBS letters

authors： Folkes LK,Candeias LP

更新日期：1997-07-28 00:00:00

abstract：:The effect of platelet-derived growth factor (PDGF) on Rap1 expression was investigated in rat vascular smooth muscle cells (SMC). First, evidence for Rap1 proteins was shown by their: (i) detection in membranes using a specific anti-Rap1 antibody, (ii) typical shift in electrophoretic mobility as a consequence of red...

journal_title：FEBS letters

authors： Quarck R,Bryckaert M,Magnier C,Corvazier E,Bredoux R,de Gunzburg J,Fontenay M,Tobelem G,Enouf J

更新日期：1994-04-04 00:00:00

abstract：:The human H19 gene is a paternally imprinted oncofetal gene, highly expressed in several fetal tissues, down-regulated in nearly all adult tissues but re-expressed in carcinomas of tissues which express the gene in fetal life. It has no known protein product and till today, no function could be designated to H19 RNA. ...

journal_title：FEBS letters

authors： Ohana P,Kopf E,Bibi O,Ayesh S,Schneider T,Laster M,Tykocinski M,de Groot N,Hochberg A

更新日期：1999-07-02 00:00:00

abstract：:Stimulation of mas-oncogene transfected 401L-C3 cells by angiotensins leads to the production of inositol phosphates. This response shows dose dependence, and has an apparent rank order of potency angiotensin III greater than or equal to angiotensin II much greater than angiotensin I. Preincubation with 12-O-tetradeca...

journal_title：FEBS letters

authors： Jackson TR,Hanley MR

更新日期：1989-07-17 00:00:00

abstract：:Recombinant porcine growth hormone (rPGH) was solubilised from inclusion bodies (IB's) using either 6 M guanidinium hydrochloride (GnHCl), 7.5 M urea or by a novel method using a cationic surfactant, cetyltrimethylammonium chloride (CTAC). Circular dichroism (CD) analysis of the secondary (2 degrees) structure of the ...

journal_title：FEBS letters

authors： Puri NK,Cardamone M

更新日期：1992-07-06 00:00:00

abstract：:Fluorescence techniques were used to study conformational changes that occur in inactive E. coli 30 S ribosomal subunits during activation by heating in 12 mM Mg2+. Activation is associated with movement of a fluorophore on the 3'-end of 16 S RNA into a less polar environment and towards a probe on the cysteine thiol ...

journal_title：FEBS letters

authors： Odom OW,Stöffler G,Hardesty B

更新日期：1984-07-23 00:00:00

abstract：:The highly polymorphic major histocompatibility complex class II (MHCII) polypeptides assemble in the ER with the assistance of invariant chain (Ii) chaperone. Ii binds to the peptide-binding pocket of MHCII heterodimers. We explored the mechanism how MHCII subunits attach to Ii. Expression with single alpha or beta s...

journal_title：FEBS letters

authors： Neumann J,Koch N

更新日期：2005-11-07 00:00:00

abstract：:The effect of the myotoxic drug chlorpromazine was studied in vitro on proteins of sarcoplasmic reticulum and mitochondrial matrix of skeletal muscle in the normal mouse. Our results indicate that the drug is specific for calcium-binding proteins (calcium ATPase, calsequestrin and calmitine). Its proteolytic effect on...

journal_title：FEBS letters

authors： Lucas-Heron B,Le Ray B,Schmitt N

更新日期：1995-11-06 00:00:00

abstract：:Lipid analyses were carried out on transplantable murine Friend leukemia cell tumors, 6 h after intratumoral administration of tumor necrosis factor-alpha (TNF). The levels of the major phospholipid classes were uniformly decreased to about 70% of control values; free fatty acids were increased to about 170%; diacylgl...

journal_title：FEBS letters

authors： Bravo E,Carpinelli G,Proietti E,Belardelli F,Cantafora A,Podo F

更新日期：1990-01-29 00:00:00

abstract：:Apoptotic protease activating factor-1 (Apaf-1) is an adaptor molecule essential for caspase-9 activation. Subcellular analysis of Apaf-1 in NIH-3T3 fibroblasts and the immature murine B cell lymphoma WEHI-231 indicates that Apaf-1 is localized in the Golgi apparatus and cytoplasm. Bcl-2 overexpression in WEHI-231 cel...

journal_title：FEBS letters

authors： Ruiz-Vela A,Albar JP,Martínez CA

更新日期：2001-07-13 00:00:00

abstract：:Cyclic ADP-ribose (cADPR) is a natural metabolite of beta-NAD+ with a potent Ca2+-mobilizing activity in different cell types, including T-lymphocytes. We investigated (i) whether stimulation of T-lymphocytes with different agonists affects the intracellular concentration of cADPR, and (ii) whether the lymphocyte anti...

journal_title：FEBS letters

authors： da Silva CP,Schweitzer K,Heyer P,Malavasi F,Mayr GW,Guse AH

更新日期：1998-11-20 00:00:00

abstract：:Synthetic oligonucleotides have been used to isolate a 1.85 kb clone containing the full length coding sequence for the catalytic subunit of rabbit skeletal muscle phosphorylase kinase from a cDNA library constructed in lambda gt10. Sequence analysis of the clone predicted an amino acid sequence in agreement with a pu...

journal_title：FEBS letters

authors： da Cruz e Silva EF,Cohen PT

更新日期：1987-08-10 00:00:00

abstract：:In Xenopus prophase-blocked oocytes, it is assumed that progesterone interacts with the plasma membrane to initiate a signalling cascade that ultimately leads to MPF activation. Progesterone regulates negatively the cAMP pathway through an inhibition of adenylate cyclase. However, the mechanisms linking the initial ac...

journal_title：FEBS letters

authors： De Smedt V,Rime H,Jessus C,Ozon R

更新日期：1995-11-20 00:00:00

abstract：:We have shown by electrophoretic mobility shift assays that the nucleotide sequence CE-LPH1, centred at position -49 with respect to the transcription start site of the rat gene encoding intestinal lactase-phlorizin hydrolase, interacts in vitro with nuclear proteins present in the jejunum of suckling animals. Protein...

journal_title：FEBS letters

authors： Boukamel R,Freund JN

更新日期：1994-10-10 00:00:00

abstract：:Feeding insects introduce oral secretions (OS) into the wounded tissue of the attacked plant. Various OS-derived molecules must be involved in subsequent processes including the induction of plant defence reactions. Using the planar lipid bilayer membrane technique, isolated OS were analyzed with respect to their memb...

journal_title：FEBS letters

authors： Maischak H,Grigoriev PA,Vogel H,Boland W,Mithöfer A

更新日期：2007-03-06 00:00:00

abstract：:Proteins with a CyaB, thiamine triphosphatase domain (CYTH domain) may play a central role at the interface between nucleotide and polyphosphate metabolism. One of the plant CYTH domain-containing proteins from Brachypodium distachyon, BdTTM3, is annotated in NCBI databases as an 'adenylyl cyclase (AC)' or a 'triphosp...

journal_title：FEBS letters

authors： Świeżawska B,Duszyn M,Kwiatkowski M,Jaworski K,Pawełek A,Szmidt-Jaworska A

更新日期：2020-03-01 00:00:00

abstract：:Stable liposomes were rendered pH-sensitive by complexation to a polymer that undergoes marked temperature- and pH-dependent water solubility changes. The N-isopropylacrylamide-methacrylic acid copolymer was prepared with or without octadecyl acrylate. At pH below the phase transition of the polymer, egg phosphatidylc...

journal_title：FEBS letters

authors： Meyer O,Papahadjopoulos D,Leroux JC

更新日期：1998-01-02 00:00:00

abstract：:Golgin45 plays important roles in Golgi stack assembly and is known to bind both the Golgi stacking protein GRASP55 and Rab2 in the medial-Golgi cisternae. In this study, we sought to further characterize the cisternal adhesion complex using a proteomics approach. We report here that Acyl-CoA binding domain containing...

journal_title：FEBS letters

authors： Yue X,Bao M,Christiano R,Li S,Mei J,Zhu L,Mao F,Yue Q,Zhang P,Jing S,Rothman JE,Qian Y,Lee I

更新日期：2017-09-01 00:00:00