Abstract:
:Three clones were isolated from a lobster digestive gland cDNA library, using oligonucleotide probes based on the partial amino terminal sequence of a digestive cysteine proteinase. The cDNAs, LCP1, LCP2 and LCP3 encode preproenzymes of 322, 323 and 321 amino acid residues, and putative mature enzymes of 217, 216 and 215 residues, respectively. Calculated mature protein molecular masses are 23386 (LCP1), 29093 (LCP2) and 23255 (LCP3) Sequence alignments show that the lobster enzymes are more similar to L (55-62% identity) than H (42-44%) or B (22-24%) cathepsins. Southern analysis indicated as many as eleven genes related to the three cDNAs.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Laycock MV,MacKay RM,Di Fruscio M,Gallant JWdoi
10.1016/0014-5793(91)80847-vsubject
Has Abstractpub_date
1991-11-04 00:00:00pages
115-20issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(91)80847-Vjournal_volume
292pub_type
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