Abstract:
:Partially purified 2-methyleneglutarate mutase from Clostridium barkeri was separated from 3-methylitaconate delta-isomerase by treatment with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) followed by FPLC on the anion exchange column Mono Q in the presence of the detergent. When purified in the dark, the active mutase contained a corrinoid, most probably coenzyme B12. The enzyme was inactivated by light at the same wavelength (lambda less than 620 nm) and rate as free coenzyme B12. The rate was not influenced by oxygen or by temperature (0-37 degrees C). Reactivation of up to 50% of the original activity was achieved by incubation with coenzyme B12 and dithiothreitol. The substrates 2-methyleneglutarate (up to 40 mM) or (R)-3-methylitaconate specifically protected the enzyme from inactivation by visible light. This effect was enhanced 3-fold by raising the temperature from 0 degrees C to 37 degrees C. The data indicate that during catalysis, the Co-C bond of the coenzyme is cleaved and cannot be affected any more by light.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Michel C,Buckel Wdoi
10.1016/0014-5793(91)80370-isubject
Has Abstractpub_date
1991-04-09 00:00:00pages
108-10issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(91)80370-Ijournal_volume
281pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The four-step caffeine biosynthetic pathway includes three methylation steps that utilise S-adenosyl-L-methionine (SAM) as the methyl donor. In the process SAM is converted to S-adenosyl-L-homocysteine (SAH) which in turn is hydrolysed to L-homocysteine and adenosine. Significant amounts of radioactivity from [methyl-...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02512-1
更新日期:2001-06-15 00:00:00
abstract::Horse plasma gelsolin labelled with benzophenone-4-isothiocyanate can be photochemically cross-linked to rabbit cardiac tropomyosin. The cross-linking proceeds with greater efficiency in calcium-containing buffers. Further evidence for interaction between these proteins is provided by retention of fluorescently labell...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80738-3
更新日期:1992-08-31 00:00:00
abstract::Synaptotagmin I, the calcium sensor for neurotransmission, is palmitoylated. We have identified the palmitoylation sites as five cysteine residues located between the transmembrane and cytoplasmic regions. In contrast to wild-type synaptotagmin, the non-acylated mutant is not converted to the endoglycosidase-H-resista...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00449-6
更新日期:2003-06-05 00:00:00
abstract::The sulfoxide Ro 18-5364, a potential metabolite of the IND Ro 18-5362, is a powerful inhibitor of gastric mucosal (H+ + K+)-ATPase, decreasing enzymatic activity with an apparent Ki of 0.1 microM. Exposure of Ro 18-5364-treated gastric membranes to dithiothreitol fully restored (H+ + K+)-ATPase activity. ATP protecte...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80324-6
更新日期:1986-03-03 00:00:00
abstract::The mechanism of store-operated Ca2+ inflow in hepatocytes was investigated using fluo-3 and fura-2 to monitor changes in the concentration of intracellular free Ca2+ in single cells, and 1-(alpha-glycerophosphoryl)-myo-inositol 4,5-diphosphate, P4(5)-1-(2-nitrophenyl)ethyl ester ('caged' GPIP2) and 'caged' guanosine ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00481-1
更新日期:1994-06-13 00:00:00
abstract::The covalent binding of pyridoxal 5'-phosphate (PLP) to human serum albumin (HSA) is important in the regulation of PLP metabolism. In plasma, PLP is bound to HSA at a single high-affinity and at two or more nonspecific sites. To characterize the primary PLP binding site, HSA was incubated with [3H] PLP, and the Schif...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80073-p
更新日期:1992-02-24 00:00:00
abstract::The eye lens protein and chaperonin, alpha-crystallin, was studied by differential scanning microcalorimetry, spectroscopy and size exclusion chromatography. The thermal transition of alpha-crystallin proceeds at Ttrs = 59.8 +/- 0.6 degrees C with an enthalpy change of delta H = 336 +/- 9 kJ per mol subunit. Disagreem...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00867-8
更新日期:1996-09-16 00:00:00
abstract::We have expressed in E. coli segments of the rod portion of rabbit skeletal fast muscle myosin and compared physical properties of two different species, LMM-30 and LMM-30C'. LMM-30 consists of 263 amino acids including the original C-terminus of myosin heavy chain. LMM-30C' is colinear with LMM-30, but is devoid of 1...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80349-8
更新日期:1991-04-09 00:00:00
abstract::A unique Cu,Zn-SOD was found and isolated from plaice Paralichthys olivaceus skin. Surprisingly, the properties of purified fish skin SOD were very different from those of SOD from other sources reported so far. The purified SOD was composed of four same subunits of 16 kDa and the molecular weight of the native SOD wa...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00084-m
更新日期:1995-02-27 00:00:00
abstract::Non-alcoholic fatty liver disease is tightly associated with insulin resistance, type 2 diabetes and obesity, but the molecular links between hepatic fat accumulation and insulin resistance are not fully identified. Excessive accumulation of triglycerides (TG) is one the main characteristics of non-alcoholic fatty liv...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2007.07.084
更新日期:2008-01-09 00:00:00
abstract::Physiological and pathophysiological differences exist between the left and right kidneys; however, the molecular bases for these differences remain unknown. Since miRNAs are involved in kidney function and the development of kidney diseases, we examined their differential expression through miRNA profiling of the lef...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13644
更新日期:2020-02-01 00:00:00
abstract::Interleukin-8 (neutrophil-activating factor; NAP-1) has been crystallized by the vapour diffusion technique to give single crystals suitable for three-dimensional structural study at a resolution higher than 2.4 A. The crystals belong to the space group P3(1)21 or P3(2)21 and have unit cell dimensions a = b = 40.9 A, ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80876-k
更新日期:1990-06-04 00:00:00
abstract::Purified barley leaf ADP-glucose pyrophosphorylase, a key enzyme of the starch synthesis in the chloroplast stroma, was analysed with respect to its possible regulation by factors defining the metabolic/effector status of the chloroplast during light and dark conditions. The enzyme required 3-phosphoglyceric acid for ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00346-4
更新日期:1999-04-01 00:00:00
abstract::Selenium depletion of H4 hepatoma cells reduced cytosolic glutathione peroxidase (cGSH-Px) mRNA abundance but had no effect on phospholipid hydroperoxide glutathione peroxidase (PHGSH-Px) mRNA abundance. Actinomycin D chase experiments showed that selenium depletion had no effect on the stability of PHGSH-Px mRNA but ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00493-0
更新日期:1996-06-03 00:00:00
abstract::Myasthenia gravis (MG) is caused by autoantibodies to the acetylcholine receptor (AChR). Experiments with fetal (alpha(2)betagammadelta) and adult (alpha(2)betaepsilondelta) AChR and with recombinant subunit dimers showed that some monoclonal antibodies (mAbs) against the main immunogenic region (MIR), located on the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01980-3
更新日期:2000-09-15 00:00:00
abstract::A-to-I RNA editing can lead to recoding of pre-mRNAs with profound functional consequences for the ensuing proteins. Here we show that complement component 1, q subcomponent-like 1 (C1QL1) undergoes RNA editing in vivo causing non-synonymous amino acid substitutions in human, mouse as well as zebrafish. The major edit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.02.044
更新日期:2009-04-02 00:00:00
abstract::The pl6INK4a tumor suppressor negatively regulates progression through the G1 phase of the mammalian cell cycle. To mimic the downmodulation of p16INK4a commonly seen in cancer, we designed and characterized a hammerhead ribozyme against exon E1alpha of the murine pl6INK4a transcript. Stable expression of the ribozyme...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01089-8
更新日期:1998-09-25 00:00:00
abstract::The action of high-intensity ultraviolet pulse laser radiation on a 161 bp fragment of pBR 322 DNA (EcoRI-MspI fragment) was studied. At doses up to 5 X 10(18) photons/cm2 the N-glycosidic bond splitting is negligible. The action of hot piperidine on irradiated DNA leads to chain splitting at the residues, modified vi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80894-2
更新日期:1985-08-19 00:00:00
abstract::The induction of adipose conversion in 3T3-L1 cells by bezafibrate has been shown to be enhanced by dibutyryl-cAMP. We here report that the induction of adipose conversion in 3T3-L1 cells, by either bezafibrate and dibutyryl cAMP, or isobutylmethyxanthine alone, is associated with a very early phosphorylation of a 60-...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80725-i
更新日期:1991-07-08 00:00:00
abstract::The archaeal exosome, a protein complex responsible for phosphorolytic degradation and tailing of RNA, has an RNA-binding platform containing Rrp4, Csl4, and DnaG. Aiming to detect novel interaction partners of the exosome, we copurified Nop5, which is a part of an rRNA methylating ribonucleoprotein complex, with the ...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12915
更新日期:2017-12-01 00:00:00
abstract::In fat-cells, insulin increases synergistically the inhibitory effects of adenosine and prostaglandin E2 on adenylyl cyclase activity. When the endogenous production of these feedback inhibitors is suppressed, insulin is no more active in conditions where glycosyl inositol-phosphate which is a putative mediator of its...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80951-x
更新日期:1991-07-29 00:00:00
abstract::A variety of mutations leading to amino acid substitutions have been described in the transthyretin gene in association with different familial amyloidoses and have been implicated to be involved in the pathogenesis of amyloid deposits. However, there has been disagreement whether or not a transthyretin mutation is pr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80387-i
更新日期:1991-04-09 00:00:00
abstract::BIR domain and its containing proteins play critical roles in cell apoptosis and cell division. Here several lines of novelty were revealed based on a comprehensive evolutionary analysis of BIR domains in 11 representative organisms. First, the type II BIR domains in Survivin and Bruce showed more conservation compare...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.09.058
更新日期:2008-11-12 00:00:00
abstract::In this paper we demonstrate that phage display technology is a suitable system for studying the interaction between the high-affinity receptor for IgE (Fc epsilon RI) and IgE. The alpha subunit extracellular domains of the human receptor were expressed on the surface of filamentous phage M13 fused to the carboxyl-ter...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80226-k
更新日期:1993-08-23 00:00:00
abstract::Single molecule localization microscopy (SMLM), which can provide up to an order of magnitude improvement in spatial resolution over conventional fluorescence microscopy, has the potential to be a highly useful tool for quantitative biological experiments. It has already been used for this purpose in varied fields in ...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2014.06.014
更新日期:2014-10-01 00:00:00
abstract::Trypanosomes are unicellular parasites and like all decent parasites, they try to obtain from the host as much material as possible, including lipids. However, the needs of a parasite are not always the same as those of the host, and therefore, mostly, some biosynthetic work still has to be done by the parasite itself...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2006.07.056
更新日期:2006-10-09 00:00:00
abstract::In Alzheimer's disease and related dementias, human tau protein aggregates into paired helical filaments and neurofibrillary tangles. However, such tau aggregates have not yet been demonstrated in transgenic mouse models of the disease. One of the possible explanations would be that mouse tau has different properties ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00522-0
更新日期:1999-05-14 00:00:00
abstract::Serine/threonine phosphatases such as PP1, PP2A, and PP2B are well known to regulate the transition phase of the cell cycle. However, the function of PP2Cgamma in cell cycle progression is still unclear. In the present study, we report the characterization of PP2Cgamma in mammalian cells during the cell cycle. After r...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.10.005
更新日期:2006-11-13 00:00:00
abstract::Tyrosine O-sulfation is one of the post-translational modification processes that occur to membrane proteins and secreted proteins in eukaryotes. Tyrosylprotein sulfotransferase (TPST) is responsible for this modification, and in this report, we describe the expression pattern and the biological role of TPST-A in the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.11.044
更新日期:2005-01-03 00:00:00
abstract::The dynamics of chromatin structure are tightly regulated by multiple epigenetic mechanisms such as histone modifications and incorporation of histone variants. In the current work, differentiation of an embryonal carcinoma cell line, NT2, was induced by retinoic acid, and total histone proteins were compared througho...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.10.041
更新日期:2010-11-19 00:00:00