Abstract:
:Macrophage cyclooxygenase-2 (COX-2) transcription is mediated through the collaboration of different promoter elements. Here, the role of an overlapping cyclic AMP responsive element (CRE)/E-box was investigated. Nuclear proteins bound both the CRE and E-box, which synergized with other promoter elements to induce COX-2 transcription. Endotoxin induced binding of nuclear proteins to the CRE and E-box and each element independently induced higher COX-2 transcription levels than the overlapping CRE/E-box. Transcription factors associated with the CRE binding complex included c-Jun and CRE binding protein and with the E-box binding complex USF-1; their overexpression significantly induced COX-2 transcription. Therefore, both CRE and E-box promoter elements regulate COX-2 transcription in macrophages.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Mestre JR,Rivadeneira DE,Mackrell PJ,Duff M,Stapleton PP,Mack-Strong V,Maddali S,Smyth GP,Tanabe T,Daly JMdoi
10.1016/s0014-5793(01)02422-xkeywords:
subject
Has Abstractpub_date
2001-05-11 00:00:00pages
147-51issue
2-3eissn
0014-5793issn
1873-3468pii
S0014-5793(01)02422-Xjournal_volume
496pub_type
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