Abstract:
:Resonance Raman spectroscopy was applied to monitor the effects of chloroquine (CQ) treatment on cultures of Plasmodium falciparum trophozoites. A number of bands assigned to A(1g) and B(1g) modes characteristic of the haemozoin aggregate are reduced in intensity in the CQ-treated cells, however, no bands from the CQ are observed. The intensity changes are attributed to intermolecular drug binding of the CQ in a sandwich type complex between ferriprotoporphyrin IX (FePPIX) dimer units. It is postulated that the CQ binds via pi-pi interactions between adjacent and orientated porphyrins thereby disrupting the haemozoin aggregate and reducing excitonic interactions between adjacent haems. The results show the potential of Raman microscopy as a screening tool for FePPIX:drug interactions in live cells.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Webster GT,Tilley L,Deed S,McNaughton D,Wood BRdoi
10.1016/j.febslet.2008.02.062subject
Has Abstractpub_date
2008-04-02 00:00:00pages
1087-92issue
7eissn
0014-5793issn
1873-3468pii
S0014-5793(08)00180-4journal_volume
582pub_type
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