Abstract:
:We have previously shown that Docetaxel-induced variable degrees of apoptosis in melanoma. In this report, we studied the beta-tubulin repertoire of melanoma cell lines and show that class III beta-tubulin expression correlated with Docetaxel-resistance. Sensitive cells showed low levels of class III beta-tubulin with little microtubular incorporation, whereas class III beta-tubulin expression was higher in resistant cells and was incorporated into the cytoskeleton. As proof of concept, abrogation of class III by siRNA reverted Docetaxel-resistant cells to a sensitive phenotype, restoring the microtubular polymerisation response and promoting high levels of apoptosis through Bax activation. These results suggest that phenotypic expression of beta-tubulin class III in melanoma may help identify patients with melanoma that can respond to taxanes.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Mhaidat NM,Thorne RF,de Bock CE,Zhang XD,Hersey Pdoi
10.1016/j.febslet.2007.12.014subject
Has Abstractpub_date
2008-01-23 00:00:00pages
267-72issue
2eissn
0014-5793issn
1873-3468pii
S0014-5793(07)01273-2journal_volume
582pub_type
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