Abstract:
:The corpus of the scientific literature has reached such size that a lot of useful data, dispersed throughout millions different articles, are now hard to recover. For instance, many articles in the biological domain describe relationships between entities (gene, proteins, small molecules, etc.) yet this crucial information cannot be efficiently used because of the difficulties in retrieving it automatically from unstructured text. Databases are striving to capture this valuable information and to organize it in a structured format ready for automatic analysis. However, the current database model, based on manual curation, is not sustainable because the limited support is not compatible with complete and accurate coverage of published information. Several proposals have been put forward to increase the efficiency and accuracy of the curation process. Here we present an experiment, designed by the editorial board of FEBS Letters, aimed at integrating each manuscript with a structured summary precisely reporting, with database identifiers and predefined controlled vocabularies, the protein interactions reported in the manuscript. The authors play an important role in this process as they are requested to provide structured information to be appended, in the form of human-readable paragraphs, at the end of traditional summaries. It is envisaged that the structured text will become an integral part of Medline abstracts. In 6 months time the experience gained with this experiment will form the basis for a community discussion to propose a widely accepted strategy for information storage and retrieval.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Ceol A,Chatr-Aryamontri A,Licata L,Cesareni Gdoi
10.1016/j.febslet.2008.02.071subject
Has Abstractpub_date
2008-04-09 00:00:00pages
1171-7issue
8eissn
0014-5793issn
1873-3468pii
S0014-5793(08)00196-8journal_volume
582pub_type
杂志文章,评审相关文献
FEBS LETTERS文献大全abstract::The functionality of the disintegrin-like/cysteine-rich domains of snake venom metalloproteinases (SVMPs) has been shown to reside in the cysteine-rich region, which can interact with VWA-containing proteins. Recently, the hyper-variable region (HVR) of the cysteine-rich domain was suggested to constitute a potential ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.10.034
更新日期:2008-11-26 00:00:00
abstract::The cytochrome bc1 complex is a key component of the mitochondrial respiratory chains of many eukaryotic microorganisms that are pathogenic for plants or humans, such as fungi responsible for crop diseases and Plasmodium falciparum, which causes human malaria. Cytochrome bc1 is an enzyme that contains two (ubi)quinone...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.13868
更新日期:2020-09-01 00:00:00
abstract::DNA is very stable in its double-stranded form. For many processes of DNA metabolism, such as replication, repair, recombination and transcription, the DNA has to be brought transiently into a single-stranded form. DNA helicases are enzymes capable of melting the hydrogen bonds of base pairs by using the energy of nuc...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(90)81279-w
更新日期:1990-08-01 00:00:00
abstract::The level of reduction of cytochrome a and CuA during the oxidation of ferrocytochrome c has been determined in stopped-flow experiments. Both components are partially reduced but become progressively more oxidized as the reaction proceeds. When all cytochrome c has been oxidized, CuA is also completely oxidized, wher...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80040-0
更新日期:1986-01-01 00:00:00
abstract::The plant-specific GAI-RGA-and-SCR (GRAS) family of proteins function as transcriptional regulators and play critical roles in development and signalling. Recent structural studies have shed light on the molecular functions at the structural level. The conserved GRAS domain comprises an α-helical cap and α/β core subd...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.12987
更新日期:2018-02-01 00:00:00
abstract::Human hepatocellular carcinoma (HCC) is one of the most common tumors worldwide, in which the genetic mechanisms of oncogenesis are still unclear. To investigate whether the genomic DNA copy number alterations may contribute to primary HCC, the cDNA microarray-based comparative genomic hybridization (CGH) analysis was...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.05.032
更新日期:2006-06-26 00:00:00
abstract::Sequence comparison based on Hydrophobic Cluster Analysis procedures shows that the extracellular approximately 200 amino acids domains of cytokines receptors belonging to the Cytokine/Growth hormone/Prolactin receptor family and to the Interferon one are organized in two homologous subdomains. Further, comparison of ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80437-8
更新日期:1991-04-22 00:00:00
abstract::Phospholipase D was first described in plant tissue but has recently been shown to occur in mammalian cells where it is activated by cell surface receptors. Its mode of activation by receptors in unclear. Biochemical studies suggest that it may occur downstream of other effector proteins and that small GTP-dependent r...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00366-x
更新日期:1999-04-09 00:00:00
abstract::RKIP was first identified as an inhibitor of the Raf-MEK-ERK signaling pathway. RKIP was also found to play an important role in the NF-kappaB pathway. Genetic and biochemical studies demonstrated that RKIP functioned as a scaffold protein facilitating the phosphorylation of IkappaB by upstream kinases. However, contr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.12.051
更新日期:2010-02-19 00:00:00
abstract::Neurotrophins support neuronal survival, development, and plasticity through processes requiring gene expression. We studied how vgf target gene transcription is mediated by a critical promoter region containing E-box, CCAAT and cAMP response element (CRE) sites. The p300 acetylase was present in two distinct protein ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03227-6
更新日期:2002-01-02 00:00:00
abstract::A membrane-bound phospholipase C-like hydrolase present in lysates of bloodstream forms of Trypanosoma brucei rapidly converts the membrane form of the variant surface protein to the soluble form and 1,2-dimyristoylglycerol [(1985) M.A.J. Ferguson et al. J. Biol. Chem., 260, 4963-4968]. The hydrolase is inhibited by p...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)81223-0
更新日期:1985-07-22 00:00:00
abstract::In brain tissue a spectrin-like calmodulin-binding protein calspectin, or fodrin, is concentrated in a synaptosome fraction, where most of the calspectin is associated with the synaptic membranes. This endogenous calspectin was phosphorylated by protein kinase system(s) associated with the membranes. Here, we report t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(82)81331-8
更新日期:1982-12-13 00:00:00
abstract::Osteoblastic cells have been shown to be involved in osteoclast formation through cell to cell contacts. This study was designed to examine the possible function of vascular cell adhesion molecule 1 (VCAM-1) during osteoclastogenesis. As a source for stromal cells we used the recently established mouse bone marrow str...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01495-0
更新日期:1997-01-27 00:00:00
abstract::The timely and reliable processing of auditory and vestibular information within the inner ear requires highly sophisticated sensory transduction pathways. On a cellular level, these demands are met by hair cells, which respond to sound waves - or alterations in body positioning - by releasing glutamate-filled synapti...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.13258
更新日期:2018-11-01 00:00:00
abstract::In fission yeast, the only known essential function of Ned8p is the modification of the cullin, Pcu1p, and subsequent Cullin-RING-Ligase (CRL) activation and substrate ubiquitination. We show here that a functional Pcu1p mutant, deleted for its C-terminal autoinhibitory domain, which negates the requirement of neddyla...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.04.011
更新日期:2012-05-21 00:00:00
abstract::The open reading frame YLR070c of Saccharomyces cerevisiae has high sequence similarity to S. cerevisiae sorbitol dehydrogenase and to xylitol dehydrogenase of Pichia stipitis. Overexpression of this open reading frame in S. cerevisiae resulted in xylitol dehydrogenase activity. The enzyme is specific for NADH. The fo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01016-9
更新日期:1999-08-20 00:00:00
abstract::The oxidation of free methionine to methionine sulfoxide by chemically or enzymatically generated oxygen free radicals is presented. The physiological significance of this process in living cells is suggested. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80443-x
更新日期:1987-11-16 00:00:00
abstract::CD133 is a widely used cell surface marker of cancer stem cells that plays an important role in tumor initiation and metastasis. Increasing evidence shows that CD133 is secreted to the extracellular space. However, the underlying mechanisms of CD133 secretion remain largely unknown. In this study, we report that secre...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13358
更新日期:2019-04-01 00:00:00
abstract::The lactase-phlorizin hydrolase gene was assigned to chromosome 2 by analysis of Southern blots of DNA from a panel of human-rodent cell hybrids containing characteristic sets of human chromosomes The hybridization probe used was a recently isolated cDNA clone of the human lactase-phlorizin hydrolase gene. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80352-1
更新日期:1988-11-21 00:00:00
abstract::Protein misfolding and aggregation are linked to several degenerative diseases and are responsible for the formation of bacterial inclusion bodies. Roles of molecular chaperones in promoting protein deposition have been speculated but not proven in vivo. We have investigated the involvement of individual chaperones in...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00126-1
更新日期:2003-02-27 00:00:00
abstract::Male germ cells specifically express paralogues of components of the general transcription apparatus including ALF a paralogue of TFIIAalpha/beta. We show that endogenous ALF is proteolytically cleaved to give alpha- and beta-subunits and we map the proteolytic cleavage site by mass spectrometry. Immunoprecipitations ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.04.083
更新日期:2005-06-20 00:00:00
abstract::Fibroblast growth factors (FGFs) and their receptors are expressed in a variety of mammalian tissues, playing a role in development and cell proliferation. While analyzing human sperm motility, we found that sperm treated with endo-β-galactosidase (EBG), which specifically hydrolyzes poly-N-acetyllactosamine type glyc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.07.056
更新日期:2013-10-01 00:00:00
abstract::The bacterial twin-arginine translocation (Tat) pathway is distinct from the Sec system by its remarkable capacity to export folded enzymes. To address the question whether the two systems are capable of translocating homologous enzymes catalyzing the same reaction, we cloned the tap gene encoding Thermus thermophilus...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02890-3
更新日期:2001-10-05 00:00:00
abstract::Mice are widely used for exploring obesity physiology and treatment. However, thermal biology is different between small and large mammals. In this Review, we discuss how the effect of environmental temperature must be understood to ensure applicability of mouse experiments to human obesity. At ambient environmental t...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.13070
更新日期:2018-06-01 00:00:00
abstract::The 77 kDa subunit of the polyadenylation cleavage stimulation factor (CstF77) is important in messenger RNA 3' end processing. Previously, we demonstrated that AtCstF77 interacts with AtCPSF30, the Arabidopsis ortholog of the 30 kDa subunit of the Cleavage and Polyadenylation Specificity Factor. In further dissecting...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.03.007
更新日期:2010-04-16 00:00:00
abstract::Highly purified mitochondria from potato (Solanum tuberosum L. cv. Bintje) tubers were subfractionated into a matrix fraction, an inner membrane fraction and an outer membrane fraction with minimal cross-contamination. When the matrix and inner membrane fractions were incubated with [gamma-32P]ATP only one and three p...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80835-i
更新日期:1993-12-27 00:00:00
abstract::Here, we used a reductant, N-acetyl-L-cysteine (NAC), to investigate the redox-sensitive step(s) in the signalling pathway from the tumor necrosis factor (TNF) receptor to nuclear factor kappaB (NF-kappaB). We found that NAC suppressed NF-kappaB activation triggered by TNF or by overexpression of either the TNF recept...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01464-2
更新日期:2000-04-28 00:00:00
abstract::Conserved residues of the proteolytic domain of Escherichia coli protease Lon, putative members of the classic catalytic triad (H665, H667, D676, and D743) were identified by comparison of amino acid sequences of Lon proteases. Mutant enzymes containing substitutions D676N, D743N, H665Y, and H667Y were obtained by sit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00012-x
更新日期:1998-01-30 00:00:00
abstract::Treatment of 125I-labelled high-density lipoprotein ([125I]HDL3) with monospecific polyclonal antibodies against apolipoproteins A-I and A-II resulted in a dose-dependent inhibition of the [125I]HDL3 binding to isolated human small intestine epithelial cells by 25% and 50%, respectively. Both antibodies also inhibited...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80519-m
更新日期:1992-06-01 00:00:00
abstract::To determine the amino acid residues required for the signal-transducing activity of the human c-Ha-Ras protein, we introduced point mutations at residues 45-54 near the 'effector region' (residues 32-40). We transfected PC12 cells with these mutant genes and also micro-injected the mutant proteins, bound with an unhy...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80665-p
更新日期:1991-12-09 00:00:00