Increased flux of free radicals in cells subjected to hyperthermia: detection by electron paramagnetic resonance spin trapping.

abstract：:Azide-binding to the heme-copper binuclear center of bo-type ubiquinol oxidase from Escherichia coli was investigated with Fourier-transform infrared spectroscopy. Deconvolution analyses of infrared spectra of the azide (14N3)-inhibited air-oxidized form showed a major infrared azide antisymmetric stretching band at 2...

journal_title：FEBS letters

authors： Tsubaki M,Mogi T,Hori H

更新日期：1999-04-23 00:00:00

abstract：:The present study was focused on the regulation of ecdysteroidogenesis in the Y-organ of Scylla serrata during molting cycle. A strong expression of molt-inhibiting hormone (MIH) and phosphorylation of ERK was predominantly observed in the postmolt and intermolt stages of Y-organs, whereas protein kinase C, steroidoge...

journal_title：FEBS letters

authors： Imayavaramban L,Dhayaparan D,Devaraj H

更新日期：2007-11-13 00:00:00

abstract：:The diterpene forskolin stimulated rat cardiac adenylate cyclase activity at least 20-fold and potentiated the effect of NaF. The stimulatory effect of forskolin was reduced in the presence of Gpp(NH)p. Ethanol markedly reduced the stimulation of adenylate cyclase by forskolin while potentiating NaF and Gpp(NH)p stimu...

journal_title：FEBS letters

authors： Robberecht P,Waelbroeck M,Chatelain P,Camus JC,Christophe J

更新日期：1983-04-05 00:00:00

abstract：:A recombinant antigen (p22-NS3), possessing putative HCV nucleocapsid protein (p22) and non-structural protein 3 (NS3) epitopes, was heavily expressed in E. coli and purified. The p22-NS3 purified recombinant antigen strongly reacts with sera containing human antibodies directed against p22 and NS3 providing a startin...

journal_title：FEBS letters

authors： Osborne S,Cecconato E,Griva S,Garetto F,Calogero R,Rosa C,Bonelli F

更新日期：1993-06-21 00:00:00

abstract：:Uroguanylin, a member of the guanylin peptide family, is a novel peptide regulator for intestinal salt and water transport. A cDNA encoding a precursor for rat uroguanylin was cloned from a rat jejunum cDNA library and sequenced. The precursor was 106 amino acids long and included a 21 residue putative signal peptide ...

journal_title：FEBS letters

authors： Miyazato M,Nakazato M,Matsukura S,Kangawa K,Matsuo H

更新日期：1996-12-02 00:00:00

abstract：:The cerebral deposition of 40-42 residue amyloid beta-protein (Abeta) is a characteristic of Alzheimer's disease. Cathepsin D is possibly involved in the intracellular clearance of Abeta (Hamazaki, H. (1996) FEBS Lett., in press). The present work shows that cathepsin D hydrolyzes wild-type Abeta 20 times faster than ...

journal_title：FEBS letters

authors： Hamazaki H

更新日期：1996-11-18 00:00:00

abstract：:The Ras homology (Rho) guanine nucleotide exchange factor p115-RhoGEF couples the alpha(13) heterotrimeric guanine nucleotide binding protein (G protein) subunit to Rho GTPase. Alpha(13) binds to a regulator of G protein signaling (RGS) domain in p115-RhoGEF, but the mechanism of alpha(13) activation of p115-RhoGEF is...

journal_title：FEBS letters

authors： Bhattacharyya R,Wedegaertner PB

更新日期：2003-04-10 00:00:00

abstract：:The role of the RNA-binding protein human antigen R (HuR) in hepatocarcinogenesis is still elusive. By employing short hairpin (sh)RNA-dependent knockdown approach, we demonstrate that lymphotoxin α (LTα) is a target of posttranscriptional gene regulation by HuR in hepatocellular carcinoma (HepG2) cells. Consequently,...

journal_title：FEBS letters

authors： Steinmeyer N,Doller A,Biyanee A,Brauss T,Schmid T,Pfeilschifter J,Eberhardt W

更新日期：2015-07-08 00:00:00

abstract：:We previously described that NO. leads to destruction of ZnS clusters and release of Zn2+ from various proteins including zinc finger transcription factors. To assess the relevance in living cells, we investigated, whether exogenous NO. leads to an increase of cytoplasmic and nuclear free Zn2+. L929 cells, mouse splen...

journal_title：FEBS letters

authors： Berendji D,Kolb-Bachofen V,Meyer KL,Grapenthin O,Weber H,Wahn V,Kröncke KD

更新日期：1997-03-17 00:00:00

abstract：:The verprolin family of proteins, WIP, CR16 and WIRE/WICH, has emerged as critical regulators of cytoskeletal organisation in vertebrate cells. The founding father of the family, verprolin, was originally identified in budding yeast and later shown to be needed for actin polymerisation during polarised growth and duri...

journal_title：FEBS letters

authors： Aspenström P

更新日期：2005-10-10 00:00:00

abstract：:Octopus, squid and cuttle-fish organs were examined for alcohol dehydrogenase activity. Only one form was detectable, with properties typical of mammalian class III alcohol dehydrogenase. The corresponding protein was purified from octopus and enzymatically characterized. Ion-exchange and affinity chromatography produ...

journal_title：FEBS letters

authors： Rosario Fernández M,Jörnvall H,Moreno A,Kaiser R,Parés X

更新日期：1993-08-16 00:00:00

abstract：:African horse sickness virus (AHSV), a member of the orbivirus genus of the family Reoviridae, is an insect-vectored pathogen of horses of concern to the equine industry. Studies on AHSV replication and pathogenesis have been hampered by the lack of reverse genetics allowing targeted mutation of viral genomes. We demo...

journal_title：FEBS letters

authors： Matsuo E,Celma CC,Roy P

更新日期：2010-08-04 00:00:00

abstract：:Stimulation of cultured pituitary cells from a gonadotrope lineage (alpha T3-1) by the gonadotropin-releasing hormone agonist analog [D-Trp6]GnRH (GnRH-A) resulted in a manifold increase in accumulation of phosphatidylethanol, a specific product of phospholipase D phosphatidyl transferase activity when ethanol is the ...

journal_title：FEBS letters

authors： Netiv E,Liscovitch M,Naor Z

更新日期：1991-12-16 00:00:00

abstract：:The C-terminal regions of Kv1 K+ channels show little conservation between isoforms except for the last four C-terminal residues, (E/L)TDV, which are well conserved from Drosophila to man. Deletions of the 4, 16, and 57 C-terminal residues of the human Kv1.5 channel did not affect whole cell current amplitude, midpoin...

journal_title：FEBS letters

authors： Uebele VN,Yeola SW,Snyders DJ,Tamkun MM

更新日期：1994-02-28 00:00:00

abstract：:Tumor suppressor WW domain-containing oxidoreductase (WWOX) is depleted in various cancer types. Here we report that WWOX is modified by small ubiquitin-like modifier (SUMO) proteins and represses DU145 prostate cancer tumorigenesis in a SUMOylation-dependent manner. Ectopic WWOX was shown to associate with SUMO2/3 or...

journal_title：FEBS letters

authors： Choi HJ,Park JH,Park JH,Lee KB,Oh SM

更新日期：2015-12-21 00:00:00

abstract：:Neurons grow during development and extend long axons to make contact with their targets with the help of an intrinsic program of axonal growth as well as a range of extrinsic cues and a permissive milieu. Injury events in adulthood induce some neuron types to revert to a regenerative state in the peripheral nervous s...

journal_title：FEBS letters

authors： Doron-Mandel E,Fainzilber M,Terenzio M

更新日期：2015-06-22 00:00:00

abstract：:DNA complementary to mRNA extracted from the thyroid glands of patients suffering from medullary carcinoma of the thyroid (MCT), a calcitonin-producing tumour, was inserted in the Pst site of pBR 322 by G-C tailing. The recombinant plasmids were used to transform Escherichia coli DP 50. Ampicillin-resistant clones wer...

journal_title：FEBS letters

authors： Le Moullec JM,Jullienne A,Chenais J,Lasmoles F,Guliana JM,Milhaud G,Moukhtar MS

更新日期：1984-02-13 00:00:00

abstract：:Transactive response DNA-binding protein of 43 kDa (TDP-43) regulates RNA processing, including alternative splicing of tau exon 10. Pathological TDP-43 is hyperphosphorylated. However, how do the protein phosphatase(s) (PP) regulate TDP-43 phosphorylation is unclear. Here, we found that both PP1 and PP2A were coimmun...

journal_title：FEBS letters

authors： Gu J,Wang W,Miao S,Chen F,Wu F,Hu W,Iqbal K,Gong CX,Liu F

更新日期：2018-02-01 00:00:00

abstract：:ZAP-70 is a protein tyrosine kinase (PTK) that plays a critical role in T cell activation. To study the role of ZAP-70 catalytic activity in this process, a substrate capable of distinguishing between the activities of ZAP-70 and other PTKs would be useful, especially since it has recently been shown that ZAP-70 inter...

journal_title：FEBS letters

authors： Watts JD,Brabb T,Bures EJ,Wange RL,Samelson LE,Aebersold R

更新日期：1996-12-02 00:00:00

abstract：:Epidermal growth factor (EGF) dose-dependently enhanced the induction of tyrosine aminotransferase and tryptophan oxygenase by glucocorticoids in primary cultures of adult rat hepatocytes without itself having any effect on these enzymes in the absence of glucocorticoids. The amplifications were observed even with dex...

journal_title：FEBS letters

authors： Kido H,Fukusen N,Katunuma N

更新日期：1987-11-02 00:00:00

abstract：:Myeloma cells specifically localize in the bone marrow and rarely circulate in blood. To study whether this immobilization could be partially explained by the presence of constitutively activated integrins, particularly alpha4beta1, we used the activation reporter HUTS-21 anti-beta1 mAb. These analyses showed that bet...

journal_title：FEBS letters

authors： García-Gila M,Cabañas C,García-Pardo A

更新日期：1997-12-01 00:00:00

abstract：:The tetraspanins associate with various surface molecules and with each other to build a network of molecular interactions, the tetraspanin web. The interaction of tetraspanins with each other seems to be central for the assembly of the tetraspanin web. All tetraspanins studied, CD9, CD37, CD53, CD63, CD81, CD82 and C...

journal_title：FEBS letters

authors： Charrin S,Manié S,Oualid M,Billard M,Boucheix C,Rubinstein E

更新日期：2002-04-10 00:00:00

abstract：:As a conserved non-coding RNA gene, transcripts of MALAT-1 localize predominately in the nucleus. However in G2/M cell cycle phase, MALAT-1 transcripts were surprisingly found to translocate from the nucleus into the cytoplasm. Investigation also found that in this process MALAT-1 interacts with an abundant nuclear fa...

journal_title：FEBS letters

authors： Yang F,Yi F,Han X,Du Q,Liang Z

更新日期：2013-10-01 00:00:00

abstract：:Mitogen activated protein kinase in extracts of U-937 macrophage-like cells was stimulated by LDL and oxLDL. A maximum value (161% of the basal phosphotransferase activity) was obtained after 6 min exposure to oxidized LDL (27 microgram/ml) using APRTPGGRR peptide substrate. The activatory effect was more pronounced (...

journal_title：FEBS letters

authors： Deigner HP,Claus R

更新日期：1996-05-06 00:00:00

abstract：:Tau phosphorylation plays a crucial role in microtubule stabilization and in Alzheimer's disease. To characterize the molecular mechanisms of tau binding on microtubules, we synthesized the peptide R1 (QTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQI), reproducing the first tau microtubule binding motif. We thermodynamically charac...

journal_title：FEBS letters

authors： Devred F,Douillard S,Briand C,Peyrot V

更新日期：2002-07-17 00:00:00

abstract：:alpha 1 Antitrypsin (alpha 1AT) is the main physiological inhibitor of neutrophil elastase, a serine protease which has been implicated in tissue degradation at inflammatory sites. We report here that the connective tissue metalloproteinase, stromelysin, cleaved alpha 1AT (54 kDa), producing fragments of approximately...

journal_title：FEBS letters

authors： Winyard PG,Zhang Z,Chidwick K,Blake DR,Carrell RW,Murphy G

更新日期：1991-02-11 00:00:00

abstract：:Respiratory supercomplex factor (Rcf) 1 is a membrane-bound protein that modulates the activity of cytochrome c oxidase (CytcO) in Saccharomyces cerevisiae mitochondria. To investigate this regulatory mechanism, we studied the interactions of CytcO with potassium cyanide (KCN) upon removal of Rcf1. While the addition ...

journal_title：FEBS letters

authors： Rydström Lundin C,Brzezinski P

更新日期：2017-12-01 00:00:00

abstract：:All the known members of the mouse Notch receptor family were examined for their biochemical function by interaction with a DNA binding protein RBP-Jkappa. mNotch2, mNotch3 and int3 (= mNotch4) were shown to interact with RBP-Jkappa by the GST-fusion pull down assay and dominant negative competition with Epstein Barr ...

journal_title：FEBS letters

authors： Kato H,Sakai T,Tamura K,Minoguchi S,Shirayoshi Y,Hamada Y,Tsujimoto Y,Honjo T

更新日期：1996-10-21 00:00:00

abstract：:Familial porphyria cutanea tarda (PCT) is a photocutaneous disease in which subnormal activity of uroporphyrinogen decarboxylase is observed both in the liver and red cells. Hepatic iron plays a key role in the genesis of overt biochemical and clinical PCT. In this report, we have studied the properties of 10 000-fold...

journal_title：FEBS letters

authors： Mukerji SK,Pimstone NR,Tan KT

更新日期：1985-09-23 00:00:00

abstract：:Analysis of the fate of the p185HER2 oncoprotein following activation by heregulin beta1 revealed the induction of the tyrosine-phosphorylation, down-modulation, and polyubiquitination of p185HER2. Receptor ubiquitination was suppressed in cells treated with heregulin beta1 in the presence of sodium azide, an inhibito...

journal_title：FEBS letters

authors： Magnifico A,Tagliabue E,Ardini E,Casalini P,Colnaghi MI,Ménard S

更新日期：1998-01-30 00:00:00