A reverse genetics system of African horse sickness virus reveals existence of primary replication.

Abstract:

:African horse sickness virus (AHSV), a member of the orbivirus genus of the family Reoviridae, is an insect-vectored pathogen of horses of concern to the equine industry. Studies on AHSV replication and pathogenesis have been hampered by the lack of reverse genetics allowing targeted mutation of viral genomes. We demonstrate that AHSV single-stranded RNA synthesized in vitro (core transcripts) is infectious and that there are distinct primary and secondary stages of the replication cycle. Transfection with a mixture of core transcripts from two different serotypes or a mixture of core transcripts and a T7 derived transcript resulted in the recovery of reassortant viruses. Recovery of infectious ASHV from nucleic acid will benefit investigation of the virus and the generation of attenuated vaccines.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Matsuo E,Celma CC,Roy P

doi

10.1016/j.febslet.2010.06.030

subject

Has Abstract

pub_date

2010-08-04 00:00:00

pages

3386-91

issue

15

eissn

0014-5793

issn

1873-3468

pii

S0014-5793(10)00525-9

journal_volume

584

pub_type

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