Abstract:
:Octopus, squid and cuttle-fish organs were examined for alcohol dehydrogenase activity. Only one form was detectable, with properties typical of mammalian class III alcohol dehydrogenase. The corresponding protein was purified from octopus and enzymatically characterized. Ion-exchange and affinity chromatography produced a pure protein in excellent yield (73%) after 1600-fold purification. Enzymatic parameters with several substrates were similar to those for the human class III alcohol dehydrogenase, demonstrating a largely conserved function of the enzyme through wide lines of divergence covering vertebrates, cephalopods and bacteria. The results establish the universal occurrence of class III alcohol dehydrogenase and its strictly conserved functional properties in separate living forms. The absence of other alcohol dehydrogenases in cephalopods is compatible with the emergence of the ethanol-active class I type at a later stage, in lineages leading to vertebrates.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Rosario Fernández M,Jörnvall H,Moreno A,Kaiser R,Parés Xdoi
10.1016/0014-5793(93)80934-msubject
Has Abstractpub_date
1993-08-16 00:00:00pages
235-8issue
3eissn
0014-5793issn
1873-3468pii
001457939380934Mjournal_volume
328pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::To study Bax-induced release of cytochrome c in vivo, we have expressed a cytochrome c-GFP (green fluorescent protein) fusion in Saccharomyces cerevisiae cells null for the expression of the endogenous cytochrome. We show here that cytochrome c-GFP is efficiently localised to mitochondria and able to function as an el...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01404-6
更新日期:2000-04-14 00:00:00
abstract::Nine of the cbi genes from the 17.5 kb cob operon of Salmonella typhimurium previously shown by genetic studies to be involved in the biosynthesis of cobinamide from precorrin-2, have been subcloned and expressed in Escherichia coli. Seven of the gene products were found in the soluble fraction of cell lysates and hav...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80213-z
更新日期:1992-04-13 00:00:00
abstract::The predominance of oxidative stress in many tumour cell environments provides a means to selectively target these cells via protein oxidation. The zinc fingers of transcription factors utilise cysteine thiols for structural zinc coordination. Redox control of DNA binding regulates transcription and therefore the over...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03890-5
更新日期:2003-01-30 00:00:00
abstract::Zwittermicin A (ZwA) is a hybrid polyketide-non-ribosomal peptide that is thought to be biosynthesized from five proposed building blocks, including the 2,3-diaminopropionate. Candidate genes for de novo biosynthesis of 2,3-diaminopropionate, zwa5A and zwa5B, have been identified in a previous study. In this research,...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.07.054
更新日期:2008-09-03 00:00:00
abstract::Extracellular regulated kinases (ERKs) mediate the inhibitory effect of tumor necrosis factor alpha (TNF-alpha) on uncoupling protein-1 (UCP-1), but not on lipid accumulation. TNF-alpha-induced ERK-dependent peroxisome proliferator activator receptor gamma (PPAR gamma) phosphorylation could be responsible for UCP-1 do...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02762-x
更新日期:2002-06-05 00:00:00
abstract::Both acute myeloid leukemia 1 and c-Fos are regulatory factors of hematopoietic cell differentiation. We identified that the c-fos promoter contains an acute myeloid leukemia 1 binding site at nucleotide positions -6-+14. c-fos promoter activity was induced by transient overexpression of acute myeloid leukemia 1 in Ju...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00190-8
更新日期:1999-03-05 00:00:00
abstract::The use of oryzacystatins I and II, two cysteine proteinase inhibitors naturally produced in rice grains, represents an attractive way for the control of Coleoptera insect pests. The present study was done to analyze the inhibitory effect of recombinant oryzacystatins produced in Escherichia coli as fusion proteins ag...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80320-t
更新日期:1993-09-27 00:00:00
abstract::We examined the spatial expression patterns of two orphan receptor guanylyl cyclase genes OlGC4 and OlGC5 during embryogenesis of medaka and characterized the 5' flanking region required for tissue-specific expression of OlGC4 by introducing promoter-GFP fusion constructs into medaka embryos. Expression of OlGC5 is co...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02109-8
更新日期:2000-10-20 00:00:00
abstract::Urate oxidase from Aspergillus flavus is a 135 kDa homo-tetramer which has a hydrophobic cavity buried within each monomer and located close to its active site. Crystallographic studies under moderate gas pressure and high hydrostatic pressure have shown that both gas presence and high pressure would rigidify the cavi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.03.017
更新日期:2014-05-02 00:00:00
abstract::Sarafotoxins (SRTa, SRTb and SRTc) as well as endothelin-1 (ET-1) produced vasoconstrictions in rat thoracic aorta, rat isolated perfused mesentery and pithed rat in various of extents. The potency was ET-1 greater than SRTb greater than SRTa greater than SRTc at lower doses, but SRTb greater than ET-1 greater than SR...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80120-8
更新日期:1990-01-29 00:00:00
abstract::Light stimulation of rod cells in vertebrate eyes may cause Ca2+ release from the intracellular disks. Radiolabelled tracers show that light causes a small hyperpolarization of intact disk stacks and redistribution of the ions Ca2+ and Cl-. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80337-9
更新日期:1985-03-25 00:00:00
abstract::A characteristic of the antioxidant, probucol, is its inability to inhibit apolipoprotein B fragmentation in low density lipoprotein (LDL), despite a pronounced ability to inhibit lipid oxidation on relatively lengthy exposure to Cu(II). Here we show that a short exposure of LDL to hydrogen peroxide and Cu(II) leads t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00706-3
更新日期:1994-08-08 00:00:00
abstract::Creatine kinases (CK) play a prominent role in cell energy distribution through an energy shuttle between mitochondria and other organelles. Human brain CK was cloned and overexpressed in COS-7 cells. We then deleted His-65 and/or Pro-66 situated near the center of a flexible loop as shown by X-ray crystallography on ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01614-8
更新日期:2000-06-09 00:00:00
abstract::LEA (late embryogenesis abundant) proteins are intrinsically disordered proteins that contribute to stress tolerance in plants and invertebrates. Here we show that, when both plant and animal LEA proteins are co-expressed in mammalian cells with self-aggregating polyglutamine (polyQ) proteins, they reduce aggregation ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.01.020
更新日期:2011-02-18 00:00:00
abstract::Nitrogenase is a two-component metalloenzyme that catalyzes a MgATP hydrolysis driven reduction of substrates. Aluminum fluoride plus MgADP inhibits nitrogenase by stabilizing an intermediate of the on-enzyme MgATP hydrolysis reaction. We report here the redox properties and electron paramagnetic resonance (EPR) signa...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00827-8
更新日期:1998-07-31 00:00:00
abstract::Non-alcoholic fatty liver disease is tightly associated with insulin resistance, type 2 diabetes and obesity, but the molecular links between hepatic fat accumulation and insulin resistance are not fully identified. Excessive accumulation of triglycerides (TG) is one the main characteristics of non-alcoholic fatty liv...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2007.07.084
更新日期:2008-01-09 00:00:00
abstract::Wilms tumor (WT) is a genetically heterogeneous childhood kidney tumor. Several genetic mutations have been identified in WT patients, including inactivation of WTX, somatic stabilizing CTNNB1, and p53 mutations. However, the molecular mechanisms in tumorigenesis remain largely unexplored. Stat3 is a transcription fac...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.01.012
更新日期:2013-03-18 00:00:00
abstract::(-)-Epigallocatechin-3-O-gallate (EGCG), a polyphenol in green tea, induces apoptosis in acute myeloid leukemia (AML) cells without affecting normal cells. In this study, we observed that cGMP acts as a cell death mediator of the EGCG-induced anti-AML effect through acid sphingomyelinase activation. EGCG activated the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.07.041
更新日期:2013-09-17 00:00:00
abstract::RCR cells are NRK clones in which Raf-1 production is blocked by the expression of an antisense RNA, and consequently they are refractory to transformation by various oncogenes. In RCR cells, MAP kinases (ERK1 and ERK2) were activated to an extent and in a time course similar to those of the original NRK cells, irresp...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80814-b
更新日期:1993-12-27 00:00:00
abstract::A cDNA encoding a novel plant type III polyketide synthase (PKS) was cloned from rhubarb (Rheum palmatum). A recombinant enzyme expressed in Escherichia coli accepted acetyl-CoA as a starter, carried out six successive condensations with malonyl-CoA and subsequent cyclization to yield an aromatic heptaketide, aloesone...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/S0014-5793(04)00230-3
更新日期:2004-03-26 00:00:00
abstract::Using an in vitro co-culture approach comprising cultured murine superior cervical ganglia and MC3T3-E1 osteoblast-like cells, we found that the addition of scorpion venom (SV) elicited neurite activation via intracellular Ca2+ mobilization and, after a lag period, osteoblastic Ca2+ mobilization. SV did not have any d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.11.065
更新日期:2007-12-22 00:00:00
abstract::Nuclear factor of activated T cells (NFATc4) has been implicated as a critical regulator of the cardiac development and hypertrophy. However, the mechanisms for regulating NFATc4 stability and transactivation remain unclear. We showed that NFATc4 protein was predominantly ubiquitinated through the formation of Lysine ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.11.009
更新日期:2008-12-10 00:00:00
abstract::Steroids are of universal occurrence, present variously as cell wall constituents and bioregulators. A number of bacteria, fungi, and photosynthetic vascular plants synthesize steroids that are hormonally active in the animal world. The cellular effect of such steroids in microbes and plants appears by and large to be...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(93)81570-p
更新日期:1993-05-17 00:00:00
abstract::Rho family small GTPases regulate lymphocyte migration induced by chemokines. However, how lymphocyte migration is regulated by Rho GTPases remains to be elucidated. Here, we identified FilGAP, a Rac-specific GAP, as a negative regulator of lymphocyte polarization and migration. Depletion of FilGAP in mouse pro-B BAF ...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12189
更新日期:2016-05-01 00:00:00
abstract::We have investigated the transcriptional activity of human cytomegalovirus, herpes thymidine kinase, human chorionic gonadotropin alpha, somatostatin, immunoglobulin kappa chain, alpha crystallin, albumin and interferon-beta promoters in the fission yeast Schizosaccharomyces pombe. Among these, the human cytomegalovir...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81012-d
更新日期:1990-07-30 00:00:00
abstract::We have recently developed an in vitro differentiation model of immortalized non-transformed human hepatocytes using butyrate, and observed the induction of inducible NO synthase (iNOS). In this study, we analyzed the effect of NO on the urea-synthetic capacity of these cells. The inhibition of iNOS during butyrate tr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01599-4
更新日期:2000-06-02 00:00:00
abstract::Homeodomain-interacting protein kinase 2 (HIPK2) interacts with and phosphorylates various transcription factors that are critical regulators of cell fate decisions and apoptosis during development. Here we show that lysine 25 of HIPK2 is the major sumoylation site, both in vitro and in vivo, and that the sumoylation ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.04.053
更新日期:2005-06-06 00:00:00
abstract::Cyclooxygenases (COXs) are key enzymes in the conversion of arachidonic acid into prostanoids which are involved in apoptosis and inflammation. Two distinct COXs have been identified: COX-1 which is constitutively expressed and COX-2 which is induced by different products such as tumor promoters or growth factors. Pre...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02924-6
更新日期:2001-10-12 00:00:00
abstract::Daunorubicin induces apoptosis in myeloid leukemia cells by activation of neutral sphingomyelinase and ceramide generation occurring 4-10 min after daunorubicin addition. We show here that daunorubicin is able to increase the phosphoinositide 3-kinase activity and enhance intracellular phosphoinositide 3-kinase lipid ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00631-6
更新日期:1999-06-11 00:00:00
abstract::Crystallographic analysis of a mutated form of "loopful" GH19 chitinase from rye seeds a double mutant RSC-c, in which Glu67 and Trp72 are mutated to glutamine and alanine, respectively, (RSC-c-E67Q/W72A) in complex with chitin tetrasaccharide (GlcNAc)₄ revealed that the entire substrate-binding cleft was completely o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.07.008
更新日期:2013-08-19 00:00:00