Abstract:
:A characteristic of the antioxidant, probucol, is its inability to inhibit apolipoprotein B fragmentation in low density lipoprotein (LDL), despite a pronounced ability to inhibit lipid oxidation on relatively lengthy exposure to Cu(II). Here we show that a short exposure of LDL to hydrogen peroxide and Cu(II) leads to 125I-labelled apolipoprotein B fragmentation, the production of malondialdehyde and hydroperoxides and leads to increased uptake by macrophages on subsequent culture. However, pre-loading LDL with probucol protects LDL from lipid oxidation but not protein fragmentation or macrophage uptake. The use of probucol to conduct studies on apolipoprotein B oxidation without extensive lipid oxidation may prove useful when studying LDL apolipoprotein damage on exposure to an aqueous free radical insult.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Hunt JV,Bailey JR,Schultz DL,McKay AG,Mitchinson MJdoi
10.1016/0014-5793(94)00706-3subject
Has Abstractpub_date
1994-08-08 00:00:00pages
375-9issue
3eissn
0014-5793issn
1873-3468pii
0014-5793(94)00706-3journal_volume
349pub_type
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