Abstract:
:DNA complementary to mRNA extracted from the thyroid glands of patients suffering from medullary carcinoma of the thyroid (MCT), a calcitonin-producing tumour, was inserted in the Pst site of pBR 322 by G-C tailing. The recombinant plasmids were used to transform Escherichia coli DP 50. Ampicillin-resistant clones were screened using a 32P-labelled cDNA to mRNA extracted from a case of MCT particularly rich in calcitonin (CT) mRNA. Positive clones were subsequently rescreened using a 32P poly(T) probe. Eighty clones were thus purified, and the inserts obtained by digestion with PstI were subjected to positive hybridization selection with subsequent translation in vitro. An insert stimulating synthesis of the protein and containing restriction sites compatible with the previously published complete sequence of calcitonin mRNA from rat was sequenced. This cDNA insert contained the entire coding region of 426 bp, 70 bp at the 5'-end, and 295 bp upstream from the poly(A) tail. The complete amino acid sequence of human preprocalcitonin could thus be deduced.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Le Moullec JM,Jullienne A,Chenais J,Lasmoles F,Guliana JM,Milhaud G,Moukhtar MSdoi
10.1016/0014-5793(84)80839-xsubject
Has Abstractpub_date
1984-02-13 00:00:00pages
93-7issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(84)80839-Xjournal_volume
167pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The oxysterols, 7beta-hydroxycholesterol and 7-ketocholesterol, are involved in the cytotoxicity of oxidized LDL. To elucidate their molecular mechanisms, the human promonocytic leukemia cells U937 and U4 were used. U4 cells overexpressing Bcl-2 were obtained by transfection of U937 cells. 7Beta-hydroxycholesterol and...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01473-7
更新日期:1997-12-15 00:00:00
abstract::The Escherichia coli K12 mutant gene, asnS40, coding for asparaginyl-tRNA synthetase (AsnRS) in the temperature-sensitive strain HO202, was isolated from genomic DNA using the Polymerase Chain Reaction. DNA sequencing revealed that the mutant enzyme differs from the wild-type AsnRS by two amino acids, but only the P23...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80106-q
更新日期:1992-03-24 00:00:00
abstract::In rat brain astroglia-enriched cultures long-term treatment with interleukin-1beta induces NO release and stimulation of soluble guanylyl cyclase. The cGMP formed is recovered in the extracellular medium but not in the cell monolayer. The interleukin-1beta effect is mediated by type I receptor and potentiated by inte...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03003-4
更新日期:2001-11-02 00:00:00
abstract::Botulinum neurotoxins (BoNTs), the causative agent of human botulism, are the most potent naturally occurring toxins known. BoNT/A1, the most studied BoNT, is also used as an important biopharmaceutical. In this study, the biological activity of BoNT/A1 is compared to that of BoNT/A2 using neuronal cell models. The da...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.11.045
更新日期:2011-01-03 00:00:00
abstract::We have engineered a genomic HDV ribozyme to construct several trans-acting ribozymes for use in trans to cleave target RNAs. Among the 10 different combinations attempted, only HDV88-Trans had cleavage activity on the 13-nucleotide substrate, R13, in vitro. To improve the cleavage efficiency, at least in vitro, of th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00941-6
更新日期:1996-09-30 00:00:00
abstract::Insight into the regulation of the actions of the protein-tyrosine kinases will be greatly facilitated by the full characterization of the family of protein-tyrosine phosphatases. A search for novel phosphatases resulted in the isolation of a cDNA, termed HLPR, encoding a member of the family of human receptor-like pr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81094-5
更新日期:1990-10-29 00:00:00
abstract::Homologues of the catalase-peroxidase gene katG and the gene for the non-specific DNA binding protein dpsA were identified downstream of oxyR in Burkholderia pseudomallei. Northern experiments revealed that both katG and dpsA are co-transcribed during oxidative stress. Under conditions where the katG promoter is not h...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00328-4
更新日期:2003-05-08 00:00:00
abstract::A 33-kDa protein component of the oxygen-evolving complex in photosystem II is essential for photosynthesis, and it has been believed that mutants with deletion of this 33-kDa protein are not found in higher plants. We report here the first isolation of an Arabidopsis thaliana mutant with a defect in one of the genes ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02963-0
更新日期:2002-07-17 00:00:00
abstract::The stimulatory effects of several DNA-binding basic proteins (histone and protamine) and HIV-1 Rev with arginine (Arg)-rich clusters on the activity of casein kinase II (CK-II) were investigated in vitro. It was found that recombinant Rev (rRev) and the synthetic oligo-fragments corresponding to the amino acid sequen...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00538-9
更新日期:1998-05-29 00:00:00
abstract::The effect of Rhodococcus fascians, the causal agent of leafy gall disease, on the mitotic behavior of synchronized tobacco Bright Yellow-2 (BY-2) cells was investigated. Incubation of aphidicolin-synchronized BY-2 cells with R. fascians cells specifically resulted in a broader mitotic index peak, an effect that was l...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02248-7
更新日期:2001-03-09 00:00:00
abstract::Adrenomedullin (ADM) functions as a survival factor against hypoxic cell death. However, molecular mechanisms underlying the cell survival pathway remain largely unknown. In this report, we showed that ADM suppressed reactive oxygen species (ROS) increase by inhibiting reduction of glutathione (GSH) level in hypoxia/r...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.11.063
更新日期:2010-01-04 00:00:00
abstract::When a high density of alpha1-subunit glycine receptor (GlyR) is expressed in Xenopus oocytes, two populations of channels can be distinguished according to their apparent affinity for glycine which differs 5- to 6-fold. To compare the open pore diameter of these channels, the relative permeability of formate with res...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00986-1
更新日期:1997-09-01 00:00:00
abstract::SigT is an ECF sigma factor in Streptomyces coelicolor. sigT and its putative anti-sigma factor gene rstA are located in one putative operon, and SigT could physically interact with RstA. Deletion of sigT or rstA caused accelerated morphological development and enhanced production of antibiotics, concomitant with over...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.09.025
更新日期:2009-10-06 00:00:00
abstract::The ATP synthase capacity of rat heart myocytes can be measured in sonicates of cultured cardiomyocytes. In these cells, transitions in ATP synthase capacity occur on changing to the anoxic or uncoupled state (drop in ATP synthase capacity of over 40%) or on electrically stimulating the cells to contract (rise of 70%)...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81725-9
更新日期:1989-10-09 00:00:00
abstract::The human gut microbiome refers to a highly diverse microbial ecosystem, which has a symbiotic relationship with the host. Molecular hydrogen (H2 ) and carbon dioxide (CO2 ) are generated by fermentative metabolism in anaerobic ecosystems. H2 generation and oxidation coupled to CO2 reduction to methane or acetate help...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.13064
更新日期:2018-06-01 00:00:00
abstract::We have found that SV40 infection of CV1 cells induces the synthesis of a 72 kDa protein that upon molecular cloning was shown to be the product of the hsc70 gene. The above gene product was found to be mainly virus inducible, in contrast to the hsp70 gene product which was mainly heat inducible. The two genes were fo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01210-5
更新日期:1994-12-05 00:00:00
abstract::The primary structure of a novel subunit of the mouse NMDA (N-methyl-D-aspartate) receptor channel, designated epsilon 4, has been revealed by cloning and sequencing the cDNA. The epsilon 4 subunit shares high amino acid sequence identity with the epsilon 1, epsilon 2 and epsilon 3 subunits of the mouse NMDA receptor ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81178-o
更新日期:1992-11-16 00:00:00
abstract::A rapid and highly efficient method of purifying the aa3-type cytochrome c oxidase from Rhodobacter sphaeroides has been developed. This method relies upon a six-histidine affinity tag fused to the C-terminus of subunit I, which confers to the oxidase a high affinity for Ni(2+)-nitrilotriacetic acid (NTA) agarose. The...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00626-k
更新日期:1995-07-10 00:00:00
abstract::Several protein kinase C (PKC) isoforms are expressed in human platelets. We report that PKC-delta is tyrosine phosphorylated within 30 s of platelet activation by thrombin. This correlated with a 2-3-fold increase in the kinase activity of PKC-delta relative to unstimulated platelets. The tyrosine phosphorylated PKC-...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01302-7
更新日期:1998-11-06 00:00:00
abstract::Production of glycerol and a key enzyme in glycerol production, glycerol 3-phosphate dehydrogenase (NAD+) (GPD), was studied in Saccharomyces cerevisiae cultured in basal media or media of high salinity, with glucose, raffinose or ethanol as the sole carbon source. At high salinity, glycerol production was stimulated ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80930-2
更新日期:1991-07-29 00:00:00
abstract::We have isolated a full-length cDNA (HPAsn.6) for human placenta glycosylasparaginase using a 221-bp PCR amplified fragment containing rat liver asparaginase gene sequences. The deduced amino acid sequence from the human clone showed sequence identity to both the alpha and beta subunits of the rat enzyme. The human en...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81211-6
更新日期:1990-09-03 00:00:00
abstract::Incorporation of dioleoyl N-(monomethoxy polyethyleneglycol succinyl)phosphatidylethanolamine (PEG-PE) into large unilamellar liposomes composed of egg phosphatidylcholine:cholesterol (1:1) does not significantly increase the content leakage when the liposomes are exposed to 90% human serum at 37 degrees C, yet the li...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81016-h
更新日期:1990-07-30 00:00:00
abstract::Ribonucleoprotein complexes, which contain mRNAs and their regulator proteins, carry out post-transcriptional control of gene expression. The function of many RNA-binding proteins depends on their association with cofactors. Here, we use a genomic approach to identify transcripts associated with DLC-1, a protein previ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13259
更新日期:2018-11-01 00:00:00
abstract::We present here a new set of aminoglycoside-arginine conjugates (AACs) that are either site-specific or per-arginine conjugates of paromomycin, neamine, and neomycin B as well as their structure-activity relationships. Their binding constants (KD) for TAR and RRE RNAs, measured by fluorescence anisotropy, revealed dep...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.10.038
更新日期:2004-11-19 00:00:00
abstract::A novel peptide from Centruroides noxius Hoffmann scorpion venom was isolated and sequenced. The 37 amino acid peptide belongs to the charybdotoxin sub-family (alphaKTx1) and was numbered member 11. alphaKTx1.11 has 75% sequence identity with iberiotoxin and 54% with charybdotoxin. alphaKTx1.11 revealed specificity fo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02791-0
更新日期:2001-09-21 00:00:00
abstract::Rat glutathione transferase 8-8 is one of the less abundant cytosolic glutathione transferases, accounting for approx. 1% of the total activity with 1-chloro-2,4-dinitrobenzene in liver. The enzyme is eluted at pH 6.3 upon chromatofocusing and has so far been identified in liver, kidney, lung and testis. Characteristi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80743-8
更新日期:1986-07-28 00:00:00
abstract::According to the endosymbiont hypothesis, mitochondria are descended from ancient aerobic bacteria that were engulfed by protoeukaryotic cells. Experiments described here show that a synthetic peptide corresponding to a yeast mitochondrial targeting sequence can be imported into Paracoccus denitrificans, a soil bacter...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80619-5
更新日期:1994-01-03 00:00:00
abstract::USP4, 11 and 15 are three closely related paralogues of the ubiquitin specific protease (USP) family of deubiquitinating enzymes. The DUSP domain and the UBL domain in these proteins are juxtaposed which may provide a functional unit conferring specificity. We determined the structures of the USP15 DUSP-UBL double dom...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.09.040
更新日期:2011-11-04 00:00:00
abstract::Two genome sequences of Escherichia coli K-12 substrains, one partial W3110 and one complete MG1655, have been determined by Japanese and American genome projects, respectively. In order to estimate the rate of nucleotide changes, we directly compared 2 Mb of the nucleotide sequences from these closely-related E. coli...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00481-0
更新日期:1999-04-30 00:00:00
abstract::Specific receptors for angiotensin II (AII) were expressed in albino Xenopus laevis oocytes co-injected with poly(A)+ mRNA isolated from rat adrenal cortex and the calcium-specific photoprotein, aequorin. In such oocytes, AII elicited rapid, dose-dependent rises in cytosolic free calcium with light emission responses ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)81055-x
更新日期:1988-12-05 00:00:00