Abstract:
:Two genome sequences of Escherichia coli K-12 substrains, one partial W3110 and one complete MG1655, have been determined by Japanese and American genome projects, respectively. In order to estimate the rate of nucleotide changes, we directly compared 2 Mb of the nucleotide sequences from these closely-related E. coli substrains. Given that the two substrains separated about 40 years ago, the rate of nucleotide changes was estimated to be less than 10(-7) per site per year. This rate was supported by a further comparison between partial genome sequences of E. coli and Shigella flexneri.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Itoh T,Okayama T,Hashimoto H,Takeda J,Davis RW,Mori H,Gojobori Tdoi
10.1016/s0014-5793(99)00481-0keywords:
subject
Has Abstractpub_date
1999-04-30 00:00:00pages
72-6issue
1-2eissn
0014-5793issn
1873-3468pii
S0014579399004810journal_volume
450pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::It is shown that conformational changes of receptor proteins brought about by binding of a ligand induce changes in the lipid environment of the receptor that can be monitored by fluorescent lipid probes. On this basis a new approach to studies of ligand-receptor binding is proposed. Using the interaction of the ricin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80108-9
更新日期:1986-01-06 00:00:00
abstract::In bovine adrenocortical cells, the fatty acyl chains of the phospholipids have been identified in the membranes of the different cell compartments: plasma membranes, Golgi complex and coated vesicle membranes. An increase in the total number of unsaturation in the fatty acid is demonstrated in the coated vesicle memb...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81201-2
更新日期:1987-07-13 00:00:00
abstract::Methanosarcina barkeri inserts pyrrolysine (Pyl) at an in-frame UAG codon in its monomethylamine methyltransferase gene. Pyrrolysyl-tRNA synthetase acylates Pyl onto tRNAPyl, the amber suppressor pyrrolysine Pyl tRNA. Here we show that M. barkeri Fusaro tRNAPyl can be misacylated with serine by the M. barkeri bacteria...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.08.027
更新日期:2008-10-15 00:00:00
abstract::A trisialoganglioside not found before in human tissue was isolated from showed this to be II3(NeuAc)3-LacCer or GT3. The ganglioside constituted 40% of the trisialogangliosides of human lung or 0.2 nmol ganglioside per g tissue. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80259-9
更新日期:1986-02-17 00:00:00
abstract::EVH1 (enabled VASP (vasodilator-stimulated protein) homology 1)/WH1 (WASP (Wiskott-Aldrich syndrome protein) homology 1) domains, present in Ena VASP and WASP, are protein interaction modules specialised in binding proline-rich ligands. An EVH1/WH1 domain is here identified in the recently cloned SMIF protein, a key p...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02751-5
更新日期:2002-05-22 00:00:00
abstract::Human seminal fluid contains a number of tripeptide amides with similar structures to thyrotropin releasing hormone (TRH), two of which have been identified as pGlu-Glu-Pro amide and pGlu-Phe-Pro amide. To determine whether these peptides originate in the same tissues and have the same molecular origin, TRH-immunoreac...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01468-3
更新日期:1996-01-22 00:00:00
abstract::Phosphorylation of H2AX is believed to be associated with the repair of damaged DNA. Recent findings suggest a novel function of H2AX in cellular apoptosis. Specifically, it was shown that ultraviolet A-activated JNK phosphorylates H2AX to regulate apoptosis. Here we show that serum starvation induces H2AX phosphoryla...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.06.051
更新日期:2008-08-06 00:00:00
abstract::We have studied the action of an alpha-latrotoxin (alpha-LTX) complex of two polypeptides (LTX 130 kDa and low molecular weight protein (LMWP) 8 kDa) and the action of a venom fraction containing LTX with excess LMWP on calcium influx into synaptosomes and PC12 cells as well as on [14C]GABA release from synaptosomes. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80803-3
更新日期:1993-12-27 00:00:00
abstract::Human complement component C8gamma is an unusual complement factor since it shows no homology to other complement proteins but is a member of the lipocalin superfamily. So far, it has been found exclusively in plasma, covalently linked to C8alpha by disulfide bridging. We have used dot blot and Northern blot analyses ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00228-8
更新日期:1999-03-12 00:00:00
abstract::We have identified and analyzed a 27-nucleotide sequence (U5 repressive element, designated as U5RE) at the U5 region of the human T-cell leukemia virus type I (HTLV-I) long terminal repeat (LTR) which is required for HTLV-I basal transcriptional repression. The basal promoter strength of constructs that contained del...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01243-1
更新日期:1994-12-12 00:00:00
abstract::Several catalytic properties of the hydrogenase from Scenedesmus obliquus have been examined to optimize the purification conditions. The Km-value for H2-evolution in the presence of the most effective electron mediator methylviologen is 0.66 mM. The pH-optimum is 6.3, the temperature-optimum is 50 degrees C and the e...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81030-4
更新日期:1993-07-19 00:00:00
abstract::Epichloë festucae Fl1 in association with Lolium perenne synthesizes a diverse range of indole-diterpene bioprotective metabolites, including lolitrem B, a potent tremorgen. The ltm genes responsible for the synthesis of these metabolites are organized in three clusters at a single sub-telomeric locus in the genome of...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.06.035
更新日期:2012-07-30 00:00:00
abstract::TP53 belongs to a small gene family that includes, in mammals, two additional paralogs, TP63 and TP73. The p63 and p73 proteins are structurally and functionally similar to p53 and their activity as transcription factors is regulated by a wide repertoire of shared and unique post-translational modifications and intera...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.06.047
更新日期:2014-08-19 00:00:00
abstract::The structure of [Val1]gramicidin A incorporated into sodium dodecyl-d25 sulphate micelles has been studied by two-dimensional proton NMR spectroscopy. Analysis of nuclear Overhauser effects, spin-spin couplings and solvent accessibility of NH groups show that the conformation of the Na+ complex of gramicidin A in det...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80702-x
更新日期:1985-07-08 00:00:00
abstract::We have previously reported that Monad, a novel WD40 repeat protein, potentiates apoptosis induced by tumor necrosis factor-alpha(TNF-alpha) and cycloheximide (CHX). By affinity purification and mass spectrometry, we identified RNA polymerase II-associated protein 3 (RPAP3) as a binding protein of Monad. Overexpressio...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.05.041
更新日期:2008-07-09 00:00:00
abstract::Three DNA restriction fragments designated tyrT, 102-mer and 70-mer, have been used as substrates for footprinting studies using DNase I in the presence of the structurally similar antibiotics chartreusin and elsamicin A. The sequence-selective binding sites of the antibiotics can be mapped in regions which are rich i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80872-z
更新日期:1991-11-04 00:00:00
abstract::The 4-chlorobenzoyl-CoA dehalogenase catalyzes the hydrolytic dechlorination of 4-chlorobenzoyl-CoA via a two-step mechanism, namely nucleophilic aromatic substitution and ester hydrolysis. The mutation of an active-site Histidine residue has been shown to reduce the catalytic activity in both the substitution and sub...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.06.056
更新日期:2005-08-15 00:00:00
abstract::Two polypeptides antigenically related to mammalian HMG1/2 have been detected in the yeast Saccharomyces cerevisiae. One exhibits an electrophoretic mobility in the range of mammalian HMG1/2 whereas the second polypeptide comigrates with yeast HMG S4. Evidence for the presence of HMG14/17 in yeast has not been obtaine...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80322-2
更新日期:1986-03-03 00:00:00
abstract::The guanine nucleotide exchange reaction catalyzed by elongation factor Ts is proposed to arise from the intrusion of the side chains of D80 and F81 near the Mg2+ binding site in EF-Tu. D80A and F81A mutants of E. coli EF-Ts were 2-3-fold less active in promoting GDP exchange with E. coli EF-Tu while the D80AF81A muta...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00789-2
更新日期:1996-08-12 00:00:00
abstract::AoXlnR was isolated as a transcriptional activator of the major xylanase gene, xynF1, in Aspergillus oryzae. To investigate the spectrum of genes under the control of AoXlnR, expression of the xylanolytic and cellulolytic genes in an A. oryzae wild type strain, an AoxlnR disruptant and an AoXlnR overexpressed strain w...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03328-8
更新日期:2002-09-25 00:00:00
abstract::We previously isolated, by differential display and 5' RACE (rapid amplification of cDNA ends), cDNAs corresponding to genes activated following cryptogein treatment of tobacco cell suspensions, among them tcI 7 (tcI for tobacco cryptogein Induced), a gene encoding a beta-subunit of proteasome. Here, we report that tc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01084-x
更新日期:2000-01-28 00:00:00
abstract::The transdifferentiation of hepatic stellate cells into myofibroblast-like cells and the proliferation of the transdifferentiated cells are controlled by TGF-beta1. Little is known about the intracellular signal transducers of TGF-beta1. In this paper we show that in cultured hepatic stellate cells TGF-beta1 induces a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00024-0
更新日期:1997-02-10 00:00:00
abstract::Tripeptidyl peptidase I (TPP-I) is a lysosomal enzyme that cleaves tripeptides from the N-terminus of polypeptides. A comparison of TPP-I amino acid sequences with sequences derived from an EST database suggested that TPP-I is identical to a pepstatin-insensitive carboxyl proteinase of unknown specificity which is mut...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01683-4
更新日期:1999-01-25 00:00:00
abstract::The cAMP-dependent induction of 2,5-oligoadenylate (2-5A) synthetase and cAMP-dependent inhibition of 2-5A phosphodiesterase are shown. Variations in activities of cAMP-dependent protein kinase and the enzymes of 2-5A metabolism in the cells deepening into the resting state were found to be compatible with the above f...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80467-6
更新日期:1984-06-04 00:00:00
abstract::Retroviral proteinases (PRs) are essential for retrovirus infectivity but the mechanism of their activity regulation is poorly understood. We investigated possible involvement in this process of the C-terminal domain (CTD) of betaretroviral PRs. We found that the presence of CTD attenuates proteolytic activity of Maso...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.09.010
更新日期:2004-10-08 00:00:00
abstract::We have set up a model system for familial amyotrophic lateral sclerosis (FALS) by transfecting human neuroblastoma cell line SH-SY5Y with plasmids directing constitutive expression of either wild-type human Cu,Zn superoxide dismutase (Cu,ZnSOD) or a mutant of this enzyme (G93A) associated with FALS. We have tested mi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01051-x
更新日期:1997-09-08 00:00:00
abstract::Prostaglandin F2alpha, platelet-derived growth factor (PDGF) and calcium ionophore A23187 stimulated the rapid (within 25 s) generation of polyunsaturated 1,2-diacylglycerol (DAG) species, in particular 18:0/20:3n-9, 18:0/20:4n-6 and 18:0/20:5n-3, in Swiss 3T3 fibroblasts. This was followed by a second sustained phase...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00471-2
更新日期:1998-05-15 00:00:00
abstract::Transcription mediators are evolutionarily conserved from yeast to human. We previously reported the specific in vivo roles of mediators during development. Transcriptional mediators including med-6, med-7, and med-10 were shown to be involved in the regulated transcription of specific genes, but not in the transcript...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03072-1
更新日期:2001-11-23 00:00:00
abstract::Retinal dehydrogenase (RALDH) catalyzes the oxidation of retinal to all-trans and 9-cis retinoic acid, which function as ligands controlling RAR and RXR nuclear receptor-signaling pathways. We have recently shown the expression of RALDH transcript in the stomach and small intestine by reverse transcription polymerase ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00355-x
更新日期:1998-04-17 00:00:00
abstract::Hepatitis B virus X protein is a major factor in the HBV-induced disease developments. Stromal cell-derived factor-1 is a small cytokine that is strongly chemotactic for lymphocytes. We explored the role of HBx on recruitment of HBV-induced virus-nonspecific immune cells into liver. Immune cell recruitment and SDF-1 e...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.01.017
更新日期:2014-03-03 00:00:00