Abstract:
:Several catalytic properties of the hydrogenase from Scenedesmus obliquus have been examined to optimize the purification conditions. The Km-value for H2-evolution in the presence of the most effective electron mediator methylviologen is 0.66 mM. The pH-optimum is 6.3, the temperature-optimum is 50 degrees C and the energy of activation is 38.4 +/- 2 kJ.mol-1. The soluble hydrogenase from the green alga, Scenedesmus obliquus, was purified 1290-fold to homogeneity. The enzyme consists of two subunits with molecular masses of 55 kDa and 36 kDa. The molecular weight of the native enzyme, determined by gel filtration, is 150 +/- 5 kDa.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Schnackenberg J,Schulz R,Senger Hdoi
10.1016/0014-5793(93)81030-4subject
Has Abstractpub_date
1993-07-19 00:00:00pages
21-4issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(93)81030-4journal_volume
327pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Rhodopsin phosphorylation was investigated using synthetic C-terminal peptides from rhodopsin. The peptides were phosphorylated by expressed rhodopsin kinase (RK) in the presence of a photolyzed truncated rhodopsin at the C-terminus. No peptide phosphorylation was detected under dark or in conditions in which RK was i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00957-5
更新日期:1997-08-25 00:00:00
abstract::Chaperone-mediated autophagy (CMA) is a lysosomal pathway that participates in the degradation of cytosolic proteins. CMA is activated by starvation and in response to stressors that result in protein damage. The selectivity intrinsic to CMA allows for removal of damaged proteins without disturbing nearby functional o...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2009.12.025
更新日期:2010-04-02 00:00:00
abstract::Liposomes containing acyl chains incorporating azobenzene chromophores have been investigated as potential 'caging' agents for fast solute release. On photolysis, trapped marker dye can be released from gel-phase liposomes within milliseconds. Solute release is markedly sensitive to the presence of cholesterol in the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01612-9
更新日期:1999-12-10 00:00:00
abstract::Acylaminoacyl peptidase is a member of the prolyl oligopeptidase family. Amino acid sequence alignment suggests that the stabilization of the tetrahedral intermediate should be mediated by His507 rather than by a tyrosine residue found in the other family members of this serine peptidase group. The pH dependence of k(...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.06.054
更新日期:2004-07-30 00:00:00
abstract::The cytochrome P-450 content of rat hepatocytes declined rapidly over 72 h in culture, due primarily to denaturation to cytochrome P-420. Six different media were investigated for their ability to conserve cytochrome P-450 during culture, and the most successful was a modified Earle's medium. After 72 h culture in thi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80436-1
更新日期:1985-10-07 00:00:00
abstract::The determination of protein structure by NMR is restricted at molecular masses above 10 kDa by overlapping resonances. One way of overcoming this problem is to label the protein with 15N. The conventional way to record 15N spectra is to use heteronuclear multiple-quantum coherence. We present here an alternative appr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81124-x
更新日期:1989-09-25 00:00:00
abstract::Blebs of the sarcoplasmic reticulum (SR) membrane of heart muscle cells were generated after saponin perforation of the plasma membrane followed by complete hypercontraction of the cell. Although characteristic proteins of the plasma membrane, namely the beta1-adrenoreceptor and Galphai, were stained by monoclonal ant...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02862-9
更新日期:2001-09-21 00:00:00
abstract::We have used ultraviolet laser crosslinking to characterize the DNA-binding properties of highly purified yeast topoisomerase II in the absence of ATP. A single 5 ns, 20 mJ pulse of 266 nm light produced optimal crosslinking to a short DNA duplex, with an efficiency of 0.25%. An equilibrium binding constant (Keq) of 1...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00035-x
更新日期:1996-02-12 00:00:00
abstract::We have investigated the effect of botulinum neurotoxin (BoNT) C1 light chain (LC) on insulin exocytosis from the clonal beta-cell line HIT-T15. In streptolysin-O permeabilized cells, the beta-cell impermeant BoNT C1 cleaved mainly syntaxin 1 and inhibited Ca2+ as well as GTPgammaS induced exocytosis. To study the eff...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01411-7
更新日期:1997-12-08 00:00:00
abstract::The isolation of a brain peptide identical to the intestinal peptide PHI (peptide HI) is described. The peptide was isolated from porcine brain extract using a chemical assay method based on its C-terminal isoleucine amide structure. The complete amino acid sequence of the peptide was found to be: His-Ala-Asp-Gly-Val-...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80617-6
更新日期:1983-03-21 00:00:00
abstract::Curcumin, the yellow pigment from Curcuma longa, has been shown to possess tumoricidal activity. We have earlier reported the induction of apoptosis in AK-5, rat histiocytic cells by curcumin leading to the inhibition of tumor growth in vivo. In this study we have observed differential activation status in host macrop...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02089-5
更新日期:2000-10-13 00:00:00
abstract::We synthesized the zwitterionic amphiphile cholesterylphosphorylethylpyridinium. This substance activated protein kinase C (PKC) in a micelle-based assay, but in a vesicle assay it was inhibitory. An analog of this compound, in which the pyridine ring is saturated and the nitrogen methylated, showed similar behaviour ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80629-u
更新日期:1992-06-15 00:00:00
abstract::Saccharomyces cerevisiae mutant strain DTY26, lacking ACE1, the protein mediator for the induction of metallothionein gene expression, is unable to increase Cu,Zn superoxide dismutase mRNA in response to copper. In the wild-type strain DTY22 transcription of both Cu,Zn superoxide dismutase and metallothionein genes is...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80131-l
更新日期:1991-01-28 00:00:00
abstract::Interleukin-8 (neutrophil-activating factor; NAP-1) has been crystallized by the vapour diffusion technique to give single crystals suitable for three-dimensional structural study at a resolution higher than 2.4 A. The crystals belong to the space group P3(1)21 or P3(2)21 and have unit cell dimensions a = b = 40.9 A, ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80876-k
更新日期:1990-06-04 00:00:00
abstract::AoXlnR was isolated as a transcriptional activator of the major xylanase gene, xynF1, in Aspergillus oryzae. To investigate the spectrum of genes under the control of AoXlnR, expression of the xylanolytic and cellulolytic genes in an A. oryzae wild type strain, an AoxlnR disruptant and an AoXlnR overexpressed strain w...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03328-8
更新日期:2002-09-25 00:00:00
abstract::Protein deglutathionylation is mainly catalyzed by glutaredoxins (GRXs). We have analyzed the biochemical properties of four of the six different GRXs of Chlamydomonas reinhardtii. Kinetic parameters were determined for disulfide and dehydroascorbate reduction but also for deglutathionylation of artificial and protein...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.04.034
更新日期:2010-06-03 00:00:00
abstract::Analysis of the amino acid sequence of bovine brain myelin proteolipid reveals not only extensive internal homology, but also homology with portions of the myelin basic protein, the peripheral nerve myelin protein, Po, and with the small proteolipid subunit of mitochondrial ATP synthase. These results suggest that the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80732-7
更新日期:1983-09-05 00:00:00
abstract::In this study, functional proteomics was successfully applied for the characterization of circadian expressed, basic proteins. For this purpose, we have chosen the green model alga Chlamydomonas reinhardtii since its entire nuclear genome is available and it is ideally suited for biochemical enrichment procedures. Pro...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/S0014-5793(04)00051-1
更新日期:2004-02-13 00:00:00
abstract::Transglutaminase 2 (TG2) is a GTP-binding protein with transglutaminase activity. Despite advances in the characterization of TG2 functions and their impact on cellular processes, the role of TG2 in Human chronic myelogenous leukemia K562 cell line is still poorly understood. To understand the biological significance ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.10.031
更新日期:2004-11-19 00:00:00
abstract::We have isolated cDNAs encoding the glucocorticoid receptor from an androgen-dependent mouse tumor, Shionogi Carcinoma 115. The nucleotide sequence of the receptor revealed two different forms, designated as SC-GR I and SC-GR II. Both forms have a one-base substitution in the DNA binding domain of the wild-type mouse ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81339-p
更新日期:1990-11-12 00:00:00
abstract::Apomyoglobin is a widely used model for studying the molecular mechanisms of globular protein folding. This work aimed to analyze the effects of rigidity and length of loops linking protein secondary structure elements on the stability of the molten globule intermediate state. For this purpose, we studied folding/unfo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13905
更新日期:2020-10-01 00:00:00
abstract::Some members of the Pax (paired box) and Six (sine oculis homeobox) gene families function as components of a gene regulatory network controlling eye development. To investigate the early evolution of the genetic interaction between Pax and Six genes, we identified and sequenced members of the Pax and Six gene familie...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.03.027
更新日期:2007-04-17 00:00:00
abstract::The N-terminus of MDM2 proto-oncoprotein interacts with p53 and down modulates p53 activity by inhibiting transcriptional activity and promoting p53 degradation. MDMX is structurally related to MDM2 and also binds to p53. However, the function of MDMX has not been clarified yet. We found that MDM2 hetero-oligomerized ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00254-9
更新日期:1999-03-19 00:00:00
abstract::The monoclonal antibody 791T /36, prepared against a human osteogenic sarcoma cell line, 791T , reacts with a variety of human tumours and also mitogen-stimulated PBMN cells. The target antigen as expressed upon 791T cells is a monomeric plasma membrane-associated glycoprotein with an apparent Mr of 72000. By quantita...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80454-8
更新日期:1984-06-04 00:00:00
abstract::Insecticidal proteins or delta-endotoxins of Bacillus thuringiensis are highly toxic to a wide range of agronomically important pests. The toxins are formed of three structural domains. The N-terminal domain is a bundle of eight alpha-helices and is implicated in pore formation in insect midgut epithelial membranes. A...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01157-6
更新日期:1999-09-17 00:00:00
abstract::Astrocyte-elevated gene-1 (AEG-1) positively regulates tumor progression and metastasis. Here, we document that AEG-1 upregulates transcription of the membrane protein tetraspanin 8 (TSPAN8). Knocking down TSPAN8 in AEG-1-overexpressing human hepatocellular carcinoma (HCC) cells markedly inhibited invasion and migrati...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12268
更新日期:2016-08-01 00:00:00
abstract::The gatC, gatA and gatB genes encoding the three subunits of glutamyl-tRNA(Gln) amidotransferase from Acidithiobacillus ferrooxidans, an acidophilic bacterium used in bioleaching of minerals, have been cloned and expressed in Escherichia coli. As in Bacillus subtilis the three gat genes are organized in an operon-like...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02600-x
更新日期:2001-07-06 00:00:00
abstract::The in vitro methylation of the +6, +24, and -215 located 5'-CCGG-3' sequences in the late E2A promoter of adenovirus type 2 (Ad2) DNA abrogates promoter function. 5-Methyldeoxycytidine (5-mC) at positions +6 and +24 in both or either of the two DNA complements in the late E2A promoter abolishes the formation of a hig...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80391-f
更新日期:1991-04-09 00:00:00
abstract::Human parainfluenza virus type 3 (hPIV3) recognizes both α2,3- and α2,6-linked sialic acids, whereas human parainfluenza virus type 1 (hPIV1) recognizes only α2,3-linked sialic acids. To identify amino acid residues that confer α2,6-linked sialic acid recognition of hPIV3, amino acid residues in or neighboring the sia...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.03.036
更新日期:2015-05-08 00:00:00
abstract::Human aminopeptidase N carries an apical sorting signal on its ectodomain necessary for its correct transport to the apical membrane in Madin-Darby canine kidney cells. To determine whether the apical sorting signal is localized in the serine/threonine rich stalk or in the catalytic head group, anchor/stalk-minus amin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81039-o
更新日期:1992-08-10 00:00:00