Role of the conserved aspartate and phenylalanine residues in prokaryotic and mitochondrial elongation factor Ts in guanine nucleotide exchange.

abstract：:Streptozotocin (STZ)-induced diabetic animals are vulnerable to cold stress. Uncoupling proteins (UCPs) play an important role in regulating thermogenesis. We investigated the gene expressions of UCPs in brown adipose tissue (BAT), white adipose tissue (WAT), liver and gastrocnemius muscle of STZ-diabetic rats using N...

journal_title：FEBS letters

authors： Kageyama H,Suga A,Kashiba M,Oka J,Osaka T,Kashiwa T,Hirano T,Nemoto K,Namba Y,Ricquier D,Giacobino JP,Inoue S

更新日期：1998-12-04 00:00:00

abstract：:The HBx protein of hepatitis B virus is involved in deregulation of cell cycle and development of hepatocellular carcinoma. Since c-Myc also plays an important role in cell proliferation and tumor development, we studied its regulation by HBx in a human hepatoma cell line. Co-expression of HBx and c-Myc resulted in in...

journal_title：FEBS letters

authors： Kalra N,Kumar V

更新日期：2006-01-23 00:00:00

abstract：:The role of Shine-Dalgarno-like sequences in mRNAs from higher plant chloroplasts has not been analyzed experimentally so far. In vitro translation analysis has revealed that the Shine-Dalgarno-like sequence is essential for translation of tobacco chloroplast rps14 mRNA. Two RNA editing sites have been identified in t...

journal_title：FEBS letters

authors： Hirose T,Kusumegi T,Sugiura M

更新日期：1998-07-03 00:00:00

abstract：:We used a xenograft model to investigate whether the aryl hydrocarbon receptor deletion construct CDelta553 suppresses tumor growth. HeLa cells that were infected with CDelta553 expressing adenovirus (Ad553) formed very small tumors whereas the control adenovirus-infected cells formed large tumors at day 15. CDelta553...

journal_title：FEBS letters

authors： Wang D,Faridi JS,Li Y,Chan WK

更新日期：2009-09-17 00:00:00

abstract：:Parts of the amino acid sequences of horse and dromedary pancreatic ribonuclease were reinvestigated. The sequence of residues 21-25 in horse ribonuclease is Ser-Asn-Pro-Thr-Tyr or Ser-Asn-Ser-Thr-Tyr. The asparagine in the latter sequence is glycosylated. Horse ribonuclease possesses four additional amino acid residu...

journal_title：FEBS letters

authors： Beintema JJ

更新日期：1985-06-03 00:00:00

abstract：:500 MHz 1H NMR studies of human epidermal growth factor are described. The backbone resonances of the 1-48 derivative of hEGF have been assigned using two-dimensional techniques. Analysis of the type and magnitude of the observed sequential nuclear Overhauser effects and the NH-alpha CH spin-spin coupling constants al...

journal_title：FEBS letters

authors： Carver JA,Cooke RM,Esposito G,Campbell ID,Gregory H,Sheard B

更新日期：1986-09-01 00:00:00

abstract：:RB, p53 and p21(Sdi1/WAF1/Cip1) interact in the induction of G1 arrest. We established osteosarcoma cell lines in which a tetracycline-regulatable promoter controls the induction of RB, p53 and p21. By using these cell lines, we investigated whether RB, p53 or p21 regulates, in the same manner or differently, expressi...

journal_title：FEBS letters

authors： Ookawa K,Tsuchida S,Kohno T,Yokota J

更新日期：2001-06-29 00:00:00

abstract：:Cerebral deposits of beta/A4 amyloid protein is a pathologic sign of Alzheimer's disease. A synthetic partial-length (1-28) peptide of this protein contains one glutamine and two lysine residues. Here we show that this peptide can be a substrate of transglutaminase, which catalyzes cross-linking between glutamine and ...

journal_title：FEBS letters

authors： Ikura K,Takahata K,Sasaki R

更新日期：1993-07-12 00:00:00

abstract：:Na+ transport through the F0 domain of Na(+)-F1F0-ATPases involves the combined action of subunits c and a but the residues involved in Na+ liganding in subunit a are unknown. As a first step towards the identification of these residues, we have cloned and sequenced the gene encoding subunit a of the Na(+)-F1F0-ATPase...

journal_title：FEBS letters

authors： Rahlfs S,Müller V

更新日期：1999-06-18 00:00:00

abstract：:A novel potassium channel beta-subunit (Kv beta 3) was cloned from rat brain being the third member of a Kv beta subunit gene family. It is a protein of 403 amino acid residues with a 68% amino acid sequence homology to Kv beta 1.1. Kv beta 3 is primarily expressed in rat brain having a distribution distinct to those ...

journal_title：FEBS letters

authors： Heinemann SH,Rettig J,Wunder F,Pongs O

更新日期：1995-12-27 00:00:00

abstract：:Activation of the formyl peptide chemoattractant receptor (FPCR) of phagocytic cells mobilizes intracellular calcium stores and affects the plasma membrane potential. Affinity crosslinking of FPCR has demonstrated a 60-80 kDa glycoprotein, with core peptide of 32 kDa. It is not known whether functional FPCR is this si...

journal_title：FEBS letters

authors： Murphy PM,Gallin EK,Tiffany HL,Malech HL

更新日期：1990-02-26 00:00:00

abstract：:The ability of (S)-alpha-methylproline (alpha-MePro) to stabilise reverse-turn conformations in the peptide hormone bradykinin (BK = Arg1-Pro2-Pro3-Gly4-Phe5-Ser6-Pro7-Phe8-Arg9) has been investigated. Two BK analogues containing alpha-MePro at position 3 or position 7 were synthesised and their conformations in aqueo...

journal_title：FEBS letters

authors： Welsh JH,Zerbe O,von Philipsborn W,Robinson JA

更新日期：1992-02-10 00:00:00

abstract：:Cripto-1 and OCT4, expressed in stem cells and cancers, play important roles in tumorigenesis. Here, we demonstrate that Cripto-1 expression is regulated by OCT4 in human embryonic carcinoma NCCIT cells. The endogenous expression of Cripto-1 and OCT4 is significantly reduced during differentiation. Cripto-1 expression...

journal_title：FEBS letters

authors： Park SW,Do HJ,Han MH,Choi W,Kim JH

更新日期：2018-01-01 00:00:00

abstract：:Somatostatin 14 (SS 14) has been isolated from pancreatic extracts, but open gel filtration immunoreactive SS often elutes in two peaks. We isolated both peaks, but upon sequence analysis only authentic SS 14 could be identified. By further gel filtration experiments it turned out that both synthetic and extractable S...

journal_title：FEBS letters

authors： Bersani M,Johnsen AH,Holst JJ

更新日期：1991-02-25 00:00:00

abstract：:Tryptophan-rich antigens of malarial parasites interact with host molecules and play an important role in parasite survival. Merozoite expressed Plasmodium vivax tryptophan-rich antigen PvTRAg38 binds to human erythrocytes and facilitates parasite growth in a heterlologous Plasmodium falciparum culture system. Recentl...

journal_title：FEBS letters

authors： Alam MS,Rathore S,Tyagi RK,Sharma YD

更新日期：2016-01-01 00:00:00

abstract：:Laser flash-induced photovoltage responses of the D85S and D85T mutants as well as of the wild-type acid blue form are similar and reflect intraprotein charge redistribution caused by retinal isomerization. The Cl- -induced transition of all of these blue forms into purple ones is accompanied by the appearance of elec...

journal_title：FEBS letters

authors： Kalaidzidis IV,Kaulen AD

更新日期：1997-12-01 00:00:00

abstract：:Parathyroid hormone (PTH) secretion is stimulated by low extracellular calcium (Ca2+) in association with a reduction in cyosolic Ca2+, indicating that this cell type does not conform to classical models of stimulus-secretion coupling. We used the phorbol ester TPA (12-O-tetradecanoyl phorbol 13-acetate), which direct...

journal_title：FEBS letters

authors： Brown EM,Redgrave J,Thatcher J

更新日期：1984-09-17 00:00:00

abstract：:Advanced glycation end products (AGE) are known to serve as ligands for the scavenger receptors such as SR-A, CD36 and SR-BI. In the current study, we examined whether AGE is recognized by lectin-like oxidized low density lipoprotein receptor-1 (LOX-1). Cellular binding experiments revealed that AGE-bovine serum album...

journal_title：FEBS letters

authors： Jono T,Miyazaki A,Nagai R,Sawamura T,Kitamura T,Horiuchi S

更新日期：2002-01-30 00:00:00

abstract：:Activation of p53 plays a central role in the cell's response to various stress signals. We investigated whether p53 is activated upon disruption of actin microfilaments, caused by cytochalasin D (CD). We show that treatment with CD leads to accumulation of p53 in the cells and activation of p53-dependent transcriptio...

journal_title：FEBS letters

authors： Rubtsova SN,Kondratov RV,Kopnin PB,Chumakov PM,Kopnin BP,Vasiliev JM

更新日期：1998-07-03 00:00:00

abstract：:Using sensitive methods of sequence analysis including hydrophobic cluster analysis, we report here a hitherto undescribed family of modules, the BAH (bromo-adjacent homology) family, which includes proteins such as eukaryotic DNA (cytosine-5) methyltransferases, the origin recognition complex 1 (Orc1) proteins, as we...

journal_title：FEBS letters

authors： Callebaut I,Courvalin JC,Mornon JP

更新日期：1999-03-05 00:00:00

abstract：:Jasmonic acid (JA) and its derivatives are well-characterized signaling molecules in plant defense and development, but the site of their localization within plant tissue is entirely unknown. To address the question whether applied JA accumulates extracellularly or intracellularly, leaves of tomato and barley were fed...

journal_title：FEBS letters

authors： Bücking H,Förster H,Stenzel I,Miersch O,Hause B

更新日期：2004-03-26 00:00:00

abstract：:Using a prostate-specific antigen cDNA as a hybridization probe, clones containing the kallikrein genes encoding prostate-specific antigen, human glandular kallikrein-1 and pancreas/kidney kallikrein were isolated from a human genomic library. Clones containing the prostate-specific antigen gene and the human glandula...

journal_title：FEBS letters

authors： Riegman PH,Vlietstra RJ,Klaassen P,van der Korput JA,Geurts van Kessel A,Romijn JC,Trapman J

更新日期：1989-04-10 00:00:00

abstract：:32 consensus patterns for a set of functional regions and structural motifs in protein sequences were constructed. The pattern definition is heuristic and based on 11 selected steric and physicochemical properties. By comparison with these patterns, it was possible to identify, without false detection, 1532 sites in 8...

journal_title：FEBS letters

authors： Bork P

更新日期：1989-10-23 00:00:00

abstract：:A novel peptide from Centruroides noxius Hoffmann scorpion venom was isolated and sequenced. The 37 amino acid peptide belongs to the charybdotoxin sub-family (alphaKTx1) and was numbered member 11. alphaKTx1.11 has 75% sequence identity with iberiotoxin and 54% with charybdotoxin. alphaKTx1.11 revealed specificity fo...

journal_title：FEBS letters

authors： Garcia-Valdes J,Zamudio FZ,Toro L,Possani LD

更新日期：2001-09-21 00:00:00

abstract：:Novel catechin derivatives obtained from grape procyanidins and l-cysteine scavenge free radicals by hydrogen atom donation, rather than electron transfer, and reduce cell viability in A375 and M21 melanoma cells. In particular, 4beta-(S-cysteinyl)epicatechin 3-O-gallate has a free radical scavenging capacity as stron...

journal_title：FEBS letters

authors： Lozano C,Torres JL,Julià L,Jimenez A,Centelles JJ,Cascante M

更新日期：2005-08-15 00:00:00

abstract：:Light stimulation of rod cells in vertebrate eyes may cause Ca2+ release from the intracellular disks. Radiolabelled tracers show that light causes a small hyperpolarization of intact disk stacks and redistribution of the ions Ca2+ and Cl-. ...

journal_title：FEBS letters

authors： Hughes SM,Brand MD

更新日期：1985-03-25 00:00:00

abstract：:Culturing human fibroblasts in a three-dimensional collagen matrix leads to a reduction of collagen I by more than 90%, both on the level of mRNA steady-state as well as protein. In order to differentiate changes in de novo transcription and posttranscriptional control, nuclear run on assays and pulse/chase experiment...

journal_title：FEBS letters

authors： Eckes B,Mauch C,Hüppe G,Krieg T

更新日期：1993-03-01 00:00:00

abstract：:Integrin avidity is regulated by changes in the conformation of the heterodimer and cluster formation. We measured cell adhesion by integrin alpha2beta1 (CHO-alpha2) to collagen at short contact times (0.5-60s) by single cell force spectroscopy (SCFS). The adhesion increased rapidly with contact time and was further s...

journal_title：FEBS letters

authors： Tulla M,Helenius J,Jokinen J,Taubenberger A,Müller DJ,Heino J

更新日期：2008-10-15 00:00:00

abstract：:Cloned mouse delta-subtype opioid receptor (DOR1) was expressed in Xenopus oocytes to study the signal transduction. Opioid delta-agonists evoked a calcium-dependent chloride current in oocytes injected with mRNA derived from DOR1, together with that from the alpha subunit of Gi1. The delta-agonist-induced current was...

journal_title：FEBS letters

authors： Miyamae T,Fukushima N,Misu Y,Ueda H

更新日期：1993-11-01 00:00:00

abstract：:Activation of T-cells infected by HIV-1 results in activation of long terminal repeat (LTR)-dependent viral transcription and ultimately the production of infectious virus. Although full T-cell activation requires a complex series of intracellular signals, including protein kinase C activation, calcium mobilisation, a...

journal_title：FEBS letters

authors： Baldari CT,Macchia G,Massone A,Telford JL

更新日期：1992-06-15 00:00:00