Abstract:
:Na+ transport through the F0 domain of Na(+)-F1F0-ATPases involves the combined action of subunits c and a but the residues involved in Na+ liganding in subunit a are unknown. As a first step towards the identification of these residues, we have cloned and sequenced the gene encoding subunit a of the Na(+)-F1F0-ATPase of Acetobacterium woodii. This is the second sequence available now for this subunit from Na(+)-F1F0-ATPases. A comparison of subunit a from Na(+)-F1F0-ATPases with those from H(+)-translocating enzymes unraveled structural similarity in a C-terminal segment including the ultimate and penultimate transmembrane helix. Seven residues are conserved in this region and, therefore, likely to be involved in Na+ liganding.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Rahlfs S,Müller Vdoi
10.1016/s0014-5793(99)00576-1keywords:
subject
Has Abstractpub_date
1999-06-18 00:00:00pages
35-40issue
1-2eissn
0014-5793issn
1873-3468pii
S0014-5793(99)00576-1journal_volume
453pub_type
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