Sequence of subunit a of the Na(+)-translocating F1F0-ATPase of Acetobacterium woodii: proposal for residues involved in Na+ binding.

Abstract:

:Na+ transport through the F0 domain of Na(+)-F1F0-ATPases involves the combined action of subunits c and a but the residues involved in Na+ liganding in subunit a are unknown. As a first step towards the identification of these residues, we have cloned and sequenced the gene encoding subunit a of the Na(+)-F1F0-ATPase of Acetobacterium woodii. This is the second sequence available now for this subunit from Na(+)-F1F0-ATPases. A comparison of subunit a from Na(+)-F1F0-ATPases with those from H(+)-translocating enzymes unraveled structural similarity in a C-terminal segment including the ultimate and penultimate transmembrane helix. Seven residues are conserved in this region and, therefore, likely to be involved in Na+ liganding.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Rahlfs S,Müller V

doi

10.1016/s0014-5793(99)00576-1

keywords:

subject

Has Abstract

pub_date

1999-06-18 00:00:00

pages

35-40

issue

1-2

eissn

0014-5793

issn

1873-3468

pii

S0014-5793(99)00576-1

journal_volume

453

pub_type

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